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Human IFN-gamma ELISA Kit - Quantikine

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Human IFN-gamma ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IFN-gamma Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
5.69 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IFN-gamma.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human IFN-gamma in this assay. No medical histories were available for the donors used in this study. All samples measured less than the lowest human IFN-gamma standard, 15.6 pg/mL.

Cell Culture Supernates:

Peripheral blood mononuclear cells from a single donor (PBMCs; seeded at 1 x 106 /mL) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were left untreated or treated with 10 μg/mL of PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IFN-gamma.

Condition(pg/mL)
UnstimulatedND
Stimulated with PHA78,480
ND=Non-Detectable

CD4+ T cells were isolated from human PBMCs (from a single donor) using the MagCellect Human CD4+ T cell Isolation Kit (R&D Systems® Catalog# MAGH102). CD4+ T cells were then seeded at 5 x 105 /mL and cultured in ExCellerate Human T Cell Expansion Media, Xeno-Free (R&D Systems® Catalog# CCM030). T cells were left unstimulated in culture media, or cultured with 10 ng/mL GMP recombinant human (rh) IL-7 (R&D Systems® Catalog# 207-GMP) and 10 ng/mL GMP rhIL-15 (R&D Systems® Catalog# 247-GMP) and stimulated using immobilized Human CD3 epsilon Antibody (R&D Systems® Catalog# MAB100, coated at 1 ug/mL) with 5 ug/mL soluble Human CD28 Antibody (R&D Systems® Catalog# MAB342) or 25 µL Cloudz CD3/CD28 (Cloudz™ T Cell Activation Kit CD3/CD28, R&D Systems®) per mL of cultured media for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IFN-gamma.

Condition(pg/mL)
UntreatedND
Stimulated with CD3/CD28 antibodies49,500
Stimulated with Cloudz T cell activation kit186,800
ND=Non-Detectable

Sample value may vary from an individual donor.


Product Summary

The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IFN-gamma levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 2 3 1 2 3
n 20 20 20 20 20
Mean (pg/mL) 366 720 154 366 723
Standard Deviation 9.16 19.4 9.9 20.6 54
CV% 2.5 2.7 6.4 5.6 7.5

Recovery

The recovery of human IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 91 84-106
EDTA Plasma (n=4) 95 85-105
Heparin Plasma (n=4) 96 87-101
Serum (n=4) 95 90-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IFN-gamma in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IFN-gamma ELISA Linearity Curve

Scientific Data

ELISA Human IFN-gamma ELISA Standard Curve View Larger

Human IFN-gamma ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ) is an important immunomodulatory cytokine, affecting both the innate and adaptive immune systems. It was discovered in 1965 as a soluble anti-viral factor and has since been shown to promote host defense against a wide variety of pathogens (1, 2). Additionally, it has been shown to promote autophagy and apoptosis, and to have anti-proliferative, anti-angiogenic, and anti-tumorigenic properties (1, 3, 4). IFN-gamma is primarily secreted by natural killer (NK) cells (5-7), activated CD8+ T cells (8), Th1 CD4+ T cells (9), NKT cells (10, 11), and macrophages (12-16), but it has also been shown to be produced by a number of other cell types including dendritic cells (17), gamma δ T cells (18), group 1 ILCs (19), keratinocytes (20), neutrophils (21), mast cells (22), and neurons (23).

The biologically active form of IFN-gamma is a non-covalently linked homodimer (24), which binds with high affinity to IFN-gamma R1/CD119 and subsequently recruits IFN-gamma R2 to form the functional heterotetrameric receptor complex. Formation of this complex leads to phosphorylation and activation of the Janus kinases, Jak1 and Jak2, which in turn phosphorylate and activate STAT1. STAT1 homodimerizes and translocates to the nucleus where it binds to IFN-gamma -activated sequence (GAS) elements in the promoters of target genes to regulate their transcription. Many of the IFN-gamma /STAT1 target genes are transcription factors that then drive the expression of secondary response genes. Additionally, IFN-gamma signaling has been shown to activate MAPK, PI 3-K/Akt, and the NF-kappa B signaling pathways, leading to the expression of multiple other genes. IFN-gamma signaling plays a key role in host defense by promoting macrophage activation, upregulating the expression of antigen processing and presentation molecules, driving the development and activation of Th1 cells, enhancing natural killer cell activity, regulating B cell functions, and inducing the production of chemokines that promote effector cell trafficking to sites of inflammation. 

Due to its immunoregulatory activities, IFN-gamma has been used as a therapeutic agent for treating a range of bacterial, fungal, helminth, protozoan, and viral infections, immunodeficiency syndromes, multi-drug resistant tuberculosis (MDR-TB), and sepsis (1, 25-34). Additionally, it has been used as an anti-tumor agent to improve patient survival in a number of different types of cancer due to its pro-apoptotic and anti-angiogenic effects (3, 35). In contrast, IFN-gamma has been suggested to be involved in the progression of cardiac diseases as elevated levels of this cytokine have been detected in the serum of patients with chronic heart failure, as well as in atherosclerotic lesions and in myocardial tissues of patients with Chagas' cardiomyopathy (1, 36, 37). Similarly, high levels of IFN-gamma have been found in the serum and/or cerebrospinal fluid of patients with neurodegenerative diseases such as Amyotrophic lateral sclerosis and Parkinson's disease (38, 39), suggesting that IFN-gamma may also be involved in neurodegenerative disease progression and serve as a clinical biomarker. Additionally, there is recent evidence suggesting that IFN-gamma may also have context-dependent proliferative and pro-tumorigenic effects (3).

Long Name:
Interferon gamma
Entrez Gene IDs:
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names:
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Citations for Human IFN-gamma Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

219 Citations: Showing 1 - 10
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  1. Development of KSHV vaccine platforms and chimeric MHV68-K-K8.1 glycoprotein for evaluating the in vivo immunogenicity and efficacy of KSHV vaccine candidates
    Authors: Yang, W-S;Kim, D;Kang, S;Lai, C-J;Cha, I;Chang, P-C;Jung, JU;
    mBio
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. SOX13-mediated transcription of LRP11 enhances malignant properties of tumor cells and CD8+ T cell inactivation in breast cancer through the ?-catenin/PD-L1 axis
    Authors: Yang, T;Dong, Y;Wang, G;Guan, X;
    Cellular signalling
    Species: Mouse
    Sample Types: Cell Culture Supernates, Tissue Homogenates
  3. Effects of dexmedetomidine and ketorolac applied for patient?controlled analgesia on the balance of Th1/Th2 and level of VEGF in patients undergoing laparoscopic surgery for cervical cancer: A randomized controlled trial
    Authors: Ao, L;Shi, J;Gan, J;Yu, W;Du, H;
    Oncology letters
    Species: Human
    Sample Types: Serum
  4. JNJ-77242113, a highly potent, selective peptide targeting the IL-23 receptor, provides robust IL-23 pathway inhibition upon oral dosing in rats and humans
    Authors: Fourie, AM;Cheng, X;Chang, L;Greving, C;Li, X;Knight, B;Polidori, D;Patrick, A;Bains, T;Steele, R;Allen, SJ;Patch, RJ;Sun, C;Somani, S;Bhandari, A;Liu, D;Huie, K;Li, S;Rodriguez, MA;Xue, X;Kannan, A;Kosoglou, T;Sherlock, JP;Towne, J;Holland, MC;Modi, NB;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Metformin induces tumor immunogenic cell death in ovarian cancer by activating AMPK pathway
    Authors: Chen, Y;Wang, L;Chen, N;Tang, G;
    Translational oncology
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Evaluation of the Activity of Cardiac Glycosides on ROR? and ROR?T Nuclear Receptors
    Authors: Karwaciak, I;Pastwi?ska, J;Sa?kowska, A;Bachorz, RA;Ratajewski, M;
    Archives of biochemistry and biophysics
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Define Critical Parameters of Trastuzumab-Mediated ADCC Assays via Assay Optimization Processes, Focusing on the Impact of Cryopreserved Effector Cells on Assay Performance
    Authors: Peng, H;Endo, Y;Wu, WJ;
    Cancers
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Discovery of immunotherapy targets for pediatric solid and brain tumors by exon-level expression.
    Authors: Shaw, TI;Wagner, J;Tian, L;Wickman, E;Poudel, S;Wang, J;Paul, R;Koo, SC;Lu, M;Sheppard, H;Fan, Y;O'Neill, FH;Lau, CC;Zhou, X;Zhang, J;Gottschalk, S;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  9. A bispecific antibody that targets the membrane-proximal region of mesothelin and retains high anticancer activity in the presence of shed mesothelin
    Authors: Chakraborty, A;Onda, M;O'Shea, T;Wei, J;Liu, X;Bera, TK;Pastan, I;
    Molecular cancer therapeutics
    Species: Human
    Sample Types: Cell Culture Supernates
  10. A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants
    Authors: Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A;
    Microorganisms
    Species: Human
    Sample Types: Cell Culture Supernates
  11. CAR-NK Cells Generated with mRNA-LNPs Kill Tumor Target Cells In Vitro and In Vivo
    Authors: Golubovskaya, V;Sienkiewicz, J;Sun, J;Zhang, S;Huang, Y;Zhou, H;Harto, H;Xu, S;Berahovich, R;Wu, L;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Hydrophobic interactions dominate the recognition of a KRAS G12V neoantigen
    Authors: Wright, KM;DiNapoli, SR;Miller, MS;Aitana Azurmendi, P;Zhao, X;Yu, Z;Chakrabarti, M;Shi, W;Douglass, J;Hwang, MS;Hsiue, EH;Mog, BJ;Pearlman, AH;Paul, S;Konig, MF;Pardoll, DM;Bettegowda, C;Papadopoulos, N;Kinzler, KW;Vogelstein, B;Zhou, S;Gabelli, SB;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  13. BCA101 is a Tumor-Targeted Bifunctional Fusion Antibody that Simultaneously Inhibits EGFR and TGF-beta Signalling to Durably Suppress Tumor Growth
    Authors: SR Boreddy, R Nair, PK Pandey, A Kuriakose, SB Marigowda, C Dey, A Banerjee, H Kulkarni, M Sagar, SR Krishn, S Rao, M A R, V Tiwari, B Alke, PK Mv, M Shri, C Dhamne, S Patel, P Sharma, S Periyasamy, J Bhatnagar, MA Kuriakose, RB Reddy, A Suresh, S Sreenivas, N Govindappa, PR Moole, U Bughani, SL Tan, P Nair
    Cancer Research, 2023-06-02;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. mRNA-Lipid Nanoparticle (LNP) Delivery of Humanized EpCAM-CD3 Bispecific Antibody Significantly Blocks Colorectal Cancer Tumor Growth
    Authors: Golubovskaya, V;Sienkiewicz, J;Sun, J;Huang, Y;Hu, L;Zhou, H;Harto, H;Xu, S;Berahovich, R;Bodmer, W;Wu, L;
    Cancers
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Possible Correlation between Urocortin 1 (Ucn1) and Immune Parameters in Patients with Endometriosis
    Authors: Abramiuk, M;Frankowska, K;Ku?ak, K;Tarkowski, R;Mertowska, P;Mertowski, S;Grywalska, E;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  16. Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors
    Authors: G Silva, AF Rodrigues, S Ferreira, C Matos, RP Eleutério, G Marques, K Kucheryava, AR Lemos, PMF Sousa, R Castro, A Barbas, D Simão, PM Alves
    Biomolecules, 2023-03-02;13(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Key cytokines and hematological parameters in patients with uncomplicated falciparum malaria in Hodeidah, Yemen
    Authors: AG Saleh, AY Shehab, R Abdul-Ghan, AF Allam, MM Osman, HS Ibrahim, HA El-Taweel, EA Moneer, NA Hagras, HF Farag
    Cytokine, 2023-02-27;164(0):156156.
    Species: Human
    Sample Types: Plasma
  18. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
    Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
    bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. WT1 Pulsed Human CD141+ Dendritic Cell Vaccine Has High Potential in Solid Tumor-Targeted Immunotherapy
    Authors: SY Cho, SM Jeong, YJ Jeon, SJ Yang, JE Hwang, BM Yoo, HS Kim
    International Journal of Molecular Sciences, 2023-01-12;24(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Effects of Near-Infrared Light on Well-Being and Health in Human Subjects with Mild Sleep-Related Complaints: A Double-Blind, Randomized, Placebo-Controlled Study
    Authors: MC Giménez, M Luxwolda, EG Van Stipri, PP Bollen, RL Hoekman, MA Koopmans, PR Arany, MR Krames, AC Berends, RA Hut, MCM Gordijn
    Biology, 2022-12-29;12(1):.
    Species: Human
    Sample Types: Plasma
  21. Growth Arrest of Alveolar Cells in Response to Cytokines from Spike S1-Activated Macrophages: Role of IFN-gamma
    Authors: A Barilli, R Visigalli, F Ferrari, G Recchia Lu, M Soli, V Dall'Asta, BM Rotoli
    Biomedicines, 2022-12-01;10(12):.
    Species: Human
    Sample Types: Cell Culture Supernates.
  22. Anti-CD19 chimeric antigen receptor T cells secreting anti-PD-L1 single-chain variable fragment attenuate PD-L1 mediated T cell inhibition
    Authors: P Yuti, Y Wutti-In, N Sawasdee, K Kongkhla, N Phanthapho, K Choomee, T Chieochans, A Panya, M Junking, PT Yenchitsom, J Sujjitjoon
    International immunopharmacology, 2022-11-23;113(0):109442.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. MET-induced CD73 restrains STING-mediated immunogenicity of EGFR-mutant lung cancer
    Authors: R Yoshida, M Saigi, T Tani, BF Springer, H Shibata, S Kitajima, NR Mahadevan, M Campisi, W Kim, Y Kobayashi, TC Thai, K Haratani, Y Yamamoto, SK Sundararam, EH Knelson, A Vajdi, I Canadas, R Uppaluri, CP Paweletz, JJ Miret, PH Lizotte, PC Gokhale, PA Janne, DA Barbie
    Cancer Research, 2022-11-02;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Inflammaging and body composition: New insights in diabetic and hypertensive elderly men
    Authors: CV Gonçalves, IS Ribeiro, MPL Galantini, IPR Muniz, PHB Lima, GS Santos, RAA da Silva
    Experimental gerontology, 2022-10-28;170(0):112005.
    Species: Human
    Sample Types: Serum
  25. Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study
    Authors: J Milosevic, M Jurisevic, V Grbovic, I Jovanovic, N Gajovic, A Jurisic-Sk
    International Journal of Environmental Research and Public Health, 2022-09-13;19(18):.
    Species: Human
    Sample Types: Serum
  26. The Generation of Dual-Targeting Fusion Protein PD-L1/CD47 for the Inhibition of Triple-Negative Breast Cancer
    Authors: Y Bian, T Lin, T Jakos, X Xiao, J Zhu
    Biomedicines, 2022-07-30;10(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. The sweet fuel of inflammation: New perspectives on the complex web that interconnects diabetes
    Authors: MPL Galantini, IS Ribeiro, CV Gonçalves, IPR Muniz, PHB Lima, GS Santos, RAA da Silva
    Experimental gerontology, 2022-07-30;167(0):111905.
    Species: Human
    Sample Types: Serum
  28. IFNgamma-primed periodontal ligament cells regulate T-cell responses via IFNgamma-inducible mediators and ICAM-1-mediated direct cell contact
    Authors: W Singhatana, S Kitpakorns, M Toso, P Pavasant
    Royal Society open science, 2022-07-27;9(7):220056.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Surface cysteine to serine substitutions in IL-18 reduce aggregation and enhance activity
    Authors: J Saetang, N Roongsawan, S Sangkhatha, SP Voravuthik, N Sangkaew, N Prompat, T Srichana, V Tipmanee
    PeerJ, 2022-07-05;10(0):e13626.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Development of Highly Effective Anti-Mesothelin hYP218 Chimeric Antigen Receptor T Cells With Increased Tumor Infiltration and Persistence for Treating Solid Tumors
    Authors: S Tomar, J Zhang, M Khanal, J Hong, A Venugopala, Q Jiang, M Sengupta, M Miettinen, N Li, I Pastan, M Ho, R Hassan
    Molecular Cancer Therapeutics, 2022-07-05;21(7):1195-1206.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1
    Authors: N Mohan, A Agrawal, Y Shen, KL Winarski, Y Endo, M Dokmanovic, D Schmiel, J Zheng, DS Rotstein, LC Pelosof, WJ Wu
    Oncogene, 2022-06-29;14(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Optimal CD8+ T cell effector function requires costimulation-induced RNA-binding proteins that reprogram the transcript isoform landscape
    Authors: TA Karginov, A Ménoret, AT Vella
    Nature Communications, 2022-06-20;13(1):3540.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Mesothelin?specific T cell cytotoxicity against triple negative breast cancer is enhanced by 40s ribosomal protein subunit 3?treated self?differentiated dendritic cells
    Authors: N Jirapongwa, S Thongchot, W Chiraphapp, T Chieochans, D Sa-Nguanra, M Warnnissor, P Thuwajit, PT Yenchitsom, C Thuwajit
    Oncology reports, 2022-05-26;48(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Epigenetic Regulation of IFI44L Expression in Monocytes Affects the Functions of Monocyte-Derived Dendritic Cells in Systemic Lupus Erythematosus
    Authors: S Luo, R Wu, Q Li, G Zhang
    Journal of Immunology Research, 2022-05-10;2022(0):4053038.
    Species: Human
    Sample Types: Serum
  35. Short treatment of peripheral blood cells product with Fas ligand using closed automated cell processing system significantly reduces immune cell reactivity of the graft in vitro and in vivo
    Authors: G Rodionov, M Rosenzwaig, MS Tzadok, M Kvint, E Gevir, E Zorde-Khva, A Peled, S Yarkoni, A Ofer
    Bone marrow transplantation, 2022-05-10;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. HOXA-AS2 contributes to regulatory T cell proliferation and immune tolerance in glioma through the miR-302a/KDM2A/JAG1 axis
    Authors: C Zhong, B Tao, X Li, W Xiang, L Peng, T Peng, L Chen, X Xia, J You, X Yang
    Cell Death & Disease, 2022-02-18;13(2):160.
    Species: Human
    Sample Types: Tissue Homogenates
  37. Extreme altitude induces divergent mass reduction of right and left ventricle in mountain climbers
    Authors: C Udjus, I Sjaastad, U Hjørnholm, TK Tunestveit, P Hoffmann, A Hinojosa, EKS Espe, G Christense, OH Skjønsberg, KO Larsen, M Rostrup
    Physiological Reports, 2022-02-01;10(3):e15184.
    Species: Human
    Sample Types: Serum
  38. Clinical and Immunological Factors Associated with Recommended Trough Levels of Adalimumab and Infliximab in Patients with Crohn's Disease
    Authors: B Orts, A Gutierrez, L Madero, L Sempere, R Frances, P Zapater
    Frontiers in Pharmacology, 2022-01-03;12(0):795272.
    Species: Human
    Sample Types: Serum
  39. Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia
    Authors: VR Ribeiro, M Romao-Veig, PR Nunes, ML Matias, JC Peracoli, MTS Peracoli
    International immunopharmacology, 2021-11-20;0(0):108366.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
    Authors: WS Khalaf, MRA Mahmoud, WF Elkhatib, HR Hashem, WE Soliman
    Heliyon, 2021-11-16;7(11):e08409.
    Species: Human
    Sample Types: Serum
  41. Impaired Innate Immunity in Pediatric Patients Type 1 Diabetes-Focus on Toll-like Receptors Expression
    Authors: K Kurianowic, M Klatka, A Polak, A Hymos, D B?bnowska, M Podgajna, R Hrynkiewic, O Sierawska, P Nied?wiedz
    International Journal of Molecular Sciences, 2021-11-09;22(22):.
    Species: Human
    Sample Types: Plasma
  42. Improvement of Inflammation and Pain after Three Months' Exclusion Diet in Rheumatoid Arthritis Patients
    Authors: MT Guagnano, C D'Angelo, D Caniglia, P Di Giovann, E Celletti, E Sabatini, L Speranza, M Bucci, F Cipollone, R Paganelli
    Nutrients, 2021-10-09;13(10):.
    Species: Human
    Sample Types: Serum
  43. Regular physical activity reduces the effects of inflammaging in diabetic and hypertensive men
    Authors: IS Ribeiro, ÍS Pereira, MPL Galantini, DP Santos, MF Teles, IPR Muniz, GS Santos, RAA Silva
    Experimental gerontology, 2021-09-20;155(0):111558.
    Species: Human
    Sample Types: Plasma
  44. Structural engineering of chimeric antigen receptors targeting HLA-restricted neoantigens
    Authors: MS Hwang, MS Miller, P Thirawatan, J Douglass, KM Wright, EH Hsiue, BJ Mog, TY Aytenfisu, MB Murphy, P Aitana Azu, AD Skora, AH Pearlman, S Paul, SR DiNapoli, MF Konig, C Bettegowda, DM Pardoll, N Papadopoul, KW Kinzler, B Vogelstein, S Zhou, SB Gabelli
    Nature Communications, 2021-09-06;12(1):5271.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Interferon-gamma-Inducible Chemokines as Prognostic Markers for Lung Cancer
    Authors: KS Lee, WY Chung, JE Park, YJ Jung, JH Park, SS Sheen, KJ Park
    International Journal of Environmental Research and Public Health, 2021-09-04;18(17):.
    Species: Human
    Sample Types: Serum
  46. The effects of T-DXd on the expression of HLA class I and chemokines CXCL9/10/11 in HER2-overexpressing gastric cancer cells
    Authors: S Nakajima, K Mimura, T Matsumoto, AK Thar Min, M Ito, H Nakano, P Neupane, Y Kanke, H Okayama, M Saito, T Momma, Y Watanabe, H Hanayama, S Hayase, Z Saze, K Kono
    Scientific Reports, 2021-08-19;11(1):16891.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Bone Marrow Neutrophils of Multiple Myeloma Patients Exhibit Myeloid-Derived Suppressor Cell Activity
    Authors: J Petersson, S Askman, Å Pettersson, S Wichert, T Hellmark, ÅCM Johansson, M Hansson
    Journal of Immunology Research, 2021-08-06;2021(0):6344344.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. A human CD137�PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade
    Authors: C Geuijen, P Tacken, LC Wang, R Klooster, PF van Loo, J Zhou, A Mondal, YB Liu, A Kramer, T Condamine, A Volgina, LJA Hendriks, H van der Ma, E Rovers, S Engels, F Fransen, R den Blanke, V Zondag-van, A Basmeleh, W Bartelink, A Kulkarni, W Marissen, CY Huang, L Hall, S Harvey, S Kim, M Martinez, S O'Brien, E Moon, S Albelda, C Kanellopou, S Stewart, H Nastri, ABH Bakker, P Scherle, T Logtenberg, G Hollis, J de Kruif, R Huber, PA Mayes, M Throsby
    Nature Communications, 2021-07-21;12(1):4445.
    Species: Cynomolgus Monkey, Human
    Sample Types: Cell Culture Supernates
  49. Engineered extracellular vesicles for concurrent Anti-PDL1 immunotherapy and chemotherapy
    Authors: Y Chen, L Wang, M Zheng, C Zhu, G Wang, Y Xia, EJ Blumenthal, W Mao, Y Wan
    Bioactive materials, 2021-07-21;9(0):251-265.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Thermoregulation is not impaired in breast cancer survivors during moderate-intensity exercise performed in warm and hot environments
    Authors: RL Relf, BJ Lee, G Eichhorn, MS Flint, L Beale, N Maxwell
    Physiological Reports, 2021-07-01;9(14):e14968.
    Species: Human
    Sample Types: Plasma
  51. Effects of Ingesting Fucoidan Derived from Cladosiphon okamuranus Tokida on Human NK Cells: A Randomized, Double-Blind, Parallel-Group, Placebo-Controlled Pilot Study
    Authors: M Tomori, T Nagamine, T Miyamoto, M Iha
    Marine Drugs, 2021-06-15;19(6):.
    Species: Human
    Sample Types: Plasma
  52. Dose-Related Aberrant Inhibition of Intracellular Perforin Expression by S1 Subunit of Spike Glycoprotein That Contains Receptor-Binding Domain from SARS-CoV-2
    Authors: CF Huang, SM Hsieh, SC Pan, YS Huang, SC Chang
    Microorganisms, 2021-06-15;9(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Regular physical activity reduces the proinflammatory response in older women with diabetes and hypertension in the postmenopausal phase
    Authors: DPS Lopes, IS Ribeiro, DC Santos, FMS Lima, AA Santos, DSP Souza, DN Lopes, AO Prado, ÍS Pereira, DP Santos, GS Santos, RAA Silva
    Experimental gerontology, 2021-06-11;152(0):111449.
    Species: Human
    Sample Types: Plasma
  54. Numerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9
    Authors: JA Jarrell, BJ Sytsma, LH Wilson, FL Pan, KHWJ Lau, GTS Kirby, AA Lievano, RS Pawell
    Scientific Reports, 2021-06-03;11(1):11818.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Socioeconomic status, financial stress, and glucocorticoid resistance among youth with asthma: Testing the moderation effects of maternal involvement and warmth
    Authors: Y Jiang, AK Farrell, ET Tobin, HE Mair-Meije, DE Wildman, F Luca, RB Slatcher, S Zilioli
    Brain, Behavior, and Immunity, 2021-05-17;0(0):.
    Species: Human
    Sample Types: Plasma
  56. Expansion of tumor-infiltrating lymphocytes (TIL) from penile cancer patients
    Authors: AM Aydin, M Hall, BL Bunch, H Branthoove, Z Sannasardo, A Mackay, M Beatty, AA Sarnaik, JE Mullinax, PE Spiess, S Pilon-Thom
    International immunopharmacology, 2021-02-23;94(0):107481.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Cytokine Profiles of Non-Small Cell Lung Cancer Patients Treated with Concurrent Chemoradiotherapy with Regards to Radiation Pneumonitis Severity
    Authors: BK Jeong, JH Kim, MH Jung, KM Kang, YH Lee
    Journal of Clinical Medicine, 2021-02-11;10(4):.
    Species: Human
    Sample Types: Plasma
  58. The anti-tumor effects of cetuximab in combination with VTX-2337 are T cell dependent
    Authors: Y Cheng, N Borcherdin, A Ogunsakin, CD Lemke-Milt, KN Gibson-Cor, A Rajan, AB Choi, W Wongpattar, CHF Chan, AK Salem, GJ Weiner, AL Simons
    Scientific Reports, 2021-01-15;11(1):1535.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. SHP2 blockade enhances anti-tumor immunity via tumor cell intrinsic and extrinsic mechanisms
    Authors: Y Wang, M Mohseni, A Grauel, JE Diez, W Guan, S Liang, JE Choi, M Pu, D Chen, T Laszewski, S Schwartz, J Gu, L Mansur, T Burks, L Brodeur, R Velazquez, S Kovats, B Pant, G Buruzula, E Deng, JT Chen, F Sari-Sarra, C Dornelas, M Varadaraja, H Yu, C Liu, J Lim, HX Hao, X Jiang, A Malamas, MJ LaMarche, FC Geyer, M McLaughlin, C Costa, J Wagner, D Ruddy, P Jayaraman, ND Kirkpatric, P Zhang, O Iartchouk, K Aardalen, V Cremasco, G Dranoff, JA Engelman, S Silver, H Wang, WD Hastings, S Goldoni
    Scientific Reports, 2021-01-14;11(1):1399.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. The Diverse Potential of Gluten from Different Durum Wheat Varieties in Triggering Celiac Disease: A Multilevel In Vitro, Ex Vivo and In Vivo Approach
    Authors: F Gaiani, S Graziano, F Boukid, B Prandi, L Bottarelli, A Barilli, A Dossena, N Marmiroli, M Gullì, GL de'Angelis, S Sforza
    Nutrients, 2020-11-20;12(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. SARS-CoV-2 Proteins Induce IFNG in Th1 Lymphocytes Generated from CD4+ Cells from Healthy, Unexposed Polish Donors
    Authors: A Sa?kowska, I Karwaciak, K Kara?, J Dastych, M Ratajewski
    Vaccines (Basel), 2020-11-12;8(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Early expansion of myeloid-derived suppressor cells inhibits SARS-CoV-2 specific T-cell response and may predict fatal COVID-19 outcome
    Authors: A Sacchi, G Grassi, V Bordoni, P Lorenzini, E Cimini, R Casetti, E Tartaglia, L Marchioni, N Petrosillo, F Palmieri, G D'Offizi, S Notari, M Tempestill, MR Capobianch, E Nicastri, M Maeurer, A Zumla, F Locatelli, A Antinori, G Ippolito, C Agrati
    Cell Death Dis, 2020-10-27;11(10):921.
    Species: Human
    Sample Types: Plasma
  63. Trastuzumab upregulates programmed death ligand-1 expression through interaction with NK cells in gastric cancer
    Authors: K Yamashita, M Iwatsuki, N Yasuda-Yos, T Morinaga, Y Nakao, K Harada, K Eto, J Kurashige, Y Hiyoshi, T Ishimoto, Y Nagai, S Iwagami, Y Baba, Y Miyamoto, N Yoshida, JA Ajani, H Baba
    Br J Cancer, 2020-10-26;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. 'Psoriasis 1' reduces T‑lymphocyte‑mediated inflammation in patients with psoriasis by inhibiting vitamin D receptor‑mediated STAT4 inactivation.
    Authors: Gao Y, Sun W, Cha X, Wang H
    Int J Mol Med, 2020-08-06;46(4):1538-1550.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Acetylated K676 TGFBIp as a severity diagnostic blood biomarker for SARS-CoV-2 pneumonia
    Authors: HH Park, HN Kim, H Kim, Y Yoo, H Shin, EY Choi, JS Bae, W Lee
    Sci Adv, 2020-07-31;6(31):.
    Species: Human
    Sample Types: Plasma
  66. Preconditioning mesenchymal stromal cells with flagellin enhances the anti?inflammatory ability of their secretome against lipopolysaccharide?induced acute lung injury
    Authors: R Li, Y Li, X Dong
    Molecular Medicine Reports, 2020-07-28;0(0):.
    Species: Human
    Sample Types: BALF
  67. Frontline Science: AMPK regulates metabolic reprogramming necessary for interferon production in human plasmacytoid dendritic cells
    Authors: HJ Hurley, H Dewald, ZS Rothkopf, S Singh, F Jenkins, P Deb, S De, BJ Barnes, P Fitzgerald
    J. Leukoc. Biol., 2020-07-08;0(0):.
    Species: Human
    Sample Types: cell culture supernatant
  68. Reactive Myelopoiesis Triggered by Lymphodepleting Chemotherapy Limits the Efficacy of Adoptive T Cell Therapy
    Authors: P Innamarato, K Kodumudi, S Asby, B Schachner, M Hall, A Mackay, D Wiener, M Beatty, L Nagle, BC Creelan, AA Sarnaik, S Pilon-Thom
    Mol. Ther., 2020-06-24;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy
    Authors: T Zhou, W Damsky, OE Weizman, MK McGeary, KP Hartmann, CE Rosen, S Fischer, R Jackson, RA Flavell, J Wang, MF Sanmamed, MW Bosenberg, AM Ring
    Nature, 2020-06-24;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Overexpression of PD-1 on Peripheral Blood Lymphocytes in Patients with Idiopathic Pulmonary Arterial Hypertension and Its Association with High Viral Loads of Epstein-Barr Virus and Poor Clinical Parameters
    Authors: M Tomaszewsk, E Grywalska, A Tomaszewsk, P B?aszczak, M Kurzyna, J Roli?ski, G Kope?
    J Clin Med, 2020-06-24;9(6):.
    Species: Human
    Sample Types: Plasma
  71. Pro-inflammatory markers and fatigue in patients with depression: A case-control study
    Authors: B Pedraz-Pet, E Neumann, G Sammer
    Sci Rep, 2020-06-11;10(1):9494.
    Species: Human
    Sample Types: Plasma
  72. Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review
    Authors: M Herrera, C Vera, Y Keynan, ZV Rueda
    J Immunol Res, 2020-04-21;2020(0):8074183.
    Species: Human
    Sample Types: Plasma
  73. Identification of Small Molecule Enhancers of Immunotherapy for Melanoma
    Authors: C Dextras, M Dashnyam, LAM Griner, J Sundaresan, B Chim, Z Yu, S Vodnala, CR Lee, X Hu, N Southall, JJ Marugan, A Jadhav, NP Restifo, N Acquavella, M Ferrer, A Singh
    Sci Rep, 2020-03-30;10(1):5688.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. PCC0208025 (BMS202), a small molecule inhibitor of PD-L1, produces an antitumor effect in B16-F10 melanoma-bearing mice
    Authors: Z Hu, P Yu, G Du, W Wang, H Zhu, N Li, H Zhao, Z Dong, L Ye, J Tian
    PLoS ONE, 2020-03-26;15(3):e0228339.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  75. A Novel Sulfonyl-Based Small Molecule Exhibiting Anti-cancer Properties
    Authors: AE El-Kadiry, J Abusarah, YE Cui, N El-Hachem, I Hammond-Ma, H Wurtele, S Thomas, M Ahmadi, M Balood, S Talbot, M Rafei
    Front Pharmacol, 2020-03-12;11(0):237.
    Species: Human
    Sample Types: Cell Culture Supernates
  76. Breast cancer stem cell RNA-pulsed dendritic cells enhance tumor cell killing by effector T cells
    Authors: N Sumransub, N Jirapongwa, P Jamjuntra, S Thongchot, T Chieochans, PT Yenchitsom, P Thuwajit, M Warnnissor, P O-Charoenr, C Thuwajit
    Oncol Lett, 2020-01-23;19(3):2422-2430.
    Species: Human
    Sample Types: Cell Culture Supernates
  77. Evaluation of immunological, inflammatory, and oxidative stress biomarkers in gasoline station attendants
    Authors: AM Moro, E Sauer, N Brucker, MF Charão, B Gauer, SN do Nascime, G Goethel, MMMF Duarte, SC Garcia
    BMC Pharmacol Toxicol, 2019-12-19;20(0):75.
    Species: Human
    Sample Types: Serum
  78. N-Octanoyl-Dopamine inhibits cytokine production in activated T-cells and diminishes MHC-class-II expression as well as adhesion molecules in IFNgamma-stimulated endothelial cells
    Authors: BB Hofmann, N Krapp, Y Li, C De La Torr, M Sol, JD Braun, M Kolibabka, P Pallavi, BK Krämer, BA Yard, AI Kälsch
    Sci Rep, 2019-12-18;9(1):19338.
    Species: Human
    Sample Types: Cell Culture Supernates
  79. The interferon-gamma pathway is selectively up-regulated in the liver of patients with secondary hemophagocytic lymphohistiocytosis
    Authors: G Prencipe, C Bracaglia, I Caiello, A Pascarella, P Francalanc, M Pardeo, A Meneghel, G Martini, MN Rossi, A Insalaco, G Marucci, V Nobili, M Spada, F Zulian, F De Benedet
    PLoS ONE, 2019-12-17;14(12):e0226043.
    Species: Human
    Sample Types: Plasma
  80. ALDH3A1 Overexpression in Melanoma and Lung Tumors Drives Cancer Stem Cell Expansion, Impairing Immune Surveillance through Enhanced PD-L1 Output
    Authors: E Terzuoli, C Bellan, S Aversa, V Ciccone, L Morbidelli, A Giachetti, S Donnini, M Ziche
    Cancers (Basel), 2019-12-06;11(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. MUC1 as a target for CAR-T therapy in head and neck squamous cell carinoma
    Authors: Z Mei, K Zhang, AK Lam, J Huang, F Qiu, B Qiao, Y Zhang
    Cancer Med, 2019-12-04;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  82. Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer
    Authors: MJ Kim, BH Chi, JJ Yoo, YM Ju, YM Whang, IH Chang
    PLoS ONE, 2019-10-22;14(10):e0223689.
    Species: Human
    Sample Types: Cell Culture Supernates
  83. Association between body composition and inflammation: A central role of IL-17 and IL-10 in diabetic and hypertensive elderly women
    Authors: IS Ribeiro, ÍS Pereira, DP Santos, DS Lopes, AO Prado, SPM Calado, CV Gonçalves, MPL Galantini, IPR Muniz, GS Santos, RAA Silva
    Exp. Gerontol., 2019-09-10;127(0):110734.
    Species: Human
    Sample Types: Plasma
  84. LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy
    Authors: J Doll, A Moghaddam, V Daniel, B Biglari, R Heller, G Schmidmaie, TF Raven
    J Orthop, 2019-08-13;17(0):208-214.
    Species: Human
    Sample Types: Serum
  85. Hepcidin exerts a negative immunological effect in pulmonary tuberculosis without HIV co-infection, prolonging the time to culture negative
    Authors: K Tashiro, M Yamamoto, R Ushio, N Kobayashi, T Sato, M Kudo, T Kaneko
    Int. J. Infect. Dis., 2019-06-25;86(0):47-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  86. Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses
    Authors: H Yu, S Cui, Y Mei, Q Li, L Wu, S Duan, G Cai, H Zhu, B Fu, L Zhang, Z Feng, X Chen
    J Immunol Res, 2019-06-17;2019(0):2121849.
    Species: Human
    Sample Types: Cell Culture Supernates
  87. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019-06-11;5(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  88. Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome
    Authors: DE Martínez-F, JR Padilla-Gu, F Casillas-M, E Valdés-Alv, B Parra-Reyn, M Aceves-Ram, JF Muñoz-Vall, U Zalapa Flo, JC Chávez Her, Y Valle
    J Immunol Res, 2019-04-30;2019(0):8063983.
    Species: Human
    Sample Types: Plasma
  89. Low-Dose Carbon Monoxide Inhibits Rhinovirus Replication in Human Alveolar and Airway Epithelial Cells
    Authors: X Deng, H Yasuda, T Sasaki, M Yamaya
    Tohoku J. Exp. Med., 2019-04-01;247(4):215-222.
    Species: Human
    Sample Types: Cell Lysates
  90. Plasma levels of TNF-?, IL-6, IFN-?, IL-12, IL-17, IL-22, and IL-23 in achalasia, eosinophilic esophagitis (EoE), and gastroesophageal reflux disease (GERD)
    Authors: S Clayton, E Cauble, A Kumar, N Patil, D Ledford, N Kolliputi, MF Lopes-Vire, D Castell, J Richter
    BMC Gastroenterol, 2019-02-11;19(1):28.
    Species: Human
    Sample Types: Plasma
  91. The effects of highly active antiretroviral therapy on the serum levels of pro-inflammatory and anti-inflammatory cytokines in HIV infected subjects
    Authors: FN Osuji, CC Onyenekwe, JE Ahaneku, NR Ukibe
    J. Biomed. Sci., 2018-12-03;25(1):88.
    Species: Human
    Sample Types: Plasma
  92. Effects of pepsin and pepstatin on reflux tonsil hypertrophy in vitro
    Authors: JH Kim, SJ Jang, JW Yun, MH Jung, SH Woo
    PLoS ONE, 2018-11-08;13(11):e0207090.
    Species: Human
    Sample Types: Whole Cells
  93. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 2018-10-10;156(1):74-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  94. A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer's disease
    Authors: J Park, I Wetzel, I Marriott, D Dréau, C D'Avanzo, DY Kim, RE Tanzi, H Cho
    Nat. Neurosci., 2018-06-27;21(7):941-951.
    Species: Human
    Sample Types: Cell Culture Supernates
  95. DOT1L inhibition attenuates graft-versus-host disease by allogeneic T cells in adoptive immunotherapy models
    Authors: Y Kagoya, M Nakatsugaw, K Saso, T Guo, M Anczurowsk, CH Wang, MO Butler, CH Arrowsmith, N Hirano
    Nat Commun, 2018-05-15;9(1):1915.
    Species: Mouse
    Sample Types: Serum
  96. Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1? and MCP-1 in cultured human retinal pigment epithelial cells
    Authors: ZM Bian, MG Field, SG Elner, JM Kahlenberg, VM Elner
    Exp. Eye Res., 2018-02-16;170(0):29-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  97. Occupational exposure to ultrafine particles in police officers: no evidence for adverse respiratory effects
    Authors: G Jordakieva, I Grabovac, E Valic, KE Schmidt, A Graff, A Schuster, K Hoffmann-S, C Oberhuber, O Scheiner, A Goll, J Godnic-Cva
    J Occup Med Toxicol, 2018-02-03;13(0):5.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  98. Nitric Oxide Production by Myeloid Derived Suppressor Cells Plays a Role in Impairing Fc Receptor-Mediated Natural Killer Cell Function
    Authors: A Stiff, P Trikha, BL Mundy-Boss, EL McMichael, TA Mace, B Benner, K Kendra, A Campbell, S Gautam, D Abood, I Landi, V Hsu, MC Duggan, R Wesolowski, M Old, JH Howard, L Yu, N Stasik, T Olencki, N Muthusamy, S Tridandapa, JC Byrd, MA Caligiuri, WE Carson
    Clin. Cancer Res., 2018-01-23;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  99. Mesenchymal stromal cells of the bone marrow and natural killer cells: cell interactions and cross modulation
    Authors: M Najar, M Fayyad-Kaz, N Meuleman, D Bron, H Fayyad-Kaz, L Lagneaux
    J Cell Commun Signal, 2018-01-19;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  100. Social conditions and immune response in human immunodeficiency virus-seropositive pregnant women: a cross-sectional study in Brazil
    Authors: AS Sampaio, ALR Vasconcelo, CNL Morais, GTN Diniz, ALC Figueiredo, SML Montenegro
    Rev. Soc. Bras. Med. Trop., 2018-01-01;51(1):21-29.
    Species: Human
    Sample Types: Plasma
  101. Construction, Expression, and Characterization of rSEA-EGF and In Vitro Evaluation of its Antitumor Activity Against Nasopharyngeal Cancer
    Authors: X Liu, L Zeng, Z Zhao, Y Xie, S Wang, J Zhang, Y He, Z Zou, J Zhang, A Tao
    Technol. Cancer Res. Treat., 2018-01-01;17(0):1533033818762.
    Species: Human
    Sample Types: Cell Culture Supernates
  102. MMP-8 and TIMP-1 are associated to periodontal inflammation in patients with rheumatoid arthritis under methotrexate immunosuppression - First results of a cross-sectional study
    Authors: G Schmalz, I Davarpanah, J Jäger, RF Mausberg, B Krohn-Grim, J Schmidt, R Haak, U Sack, D Ziebolz
    J Microbiol Immunol Infect, 2017-09-04;0(0):.
    Species: Human
    Sample Types: Serum
  103. The in vitro and ex vivo effect of Auranta 3001 in preventing Cryptosporidium hominis and Cryptosporidium parvum infection
    Authors: A Ch Stratak, F Sima, P Ward, M Linton, C Kelly, L Pinkerton, L Stef, I Pet, T Iancu, G Pircalabio, N Corcionivo
    Gut Pathog, 2017-08-31;9(0):49.
    Species: Bovine, Human
    Sample Types: Cell Culture Supernates
  104. T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab
    Authors: U Bughani, A Saha, A Kuriakose, R Nair, RB Sadashivar, R Venkataram, S Patel, AT Deshchougu, SK S, E Montero, HV Pai, DV Palanivelu, R Melarkode, P Nair
    PLoS ONE, 2017-07-03;12(7):e0180088.
    Species: Human
    Sample Types: Cell Culture Supernates
  105. Association between cytokine profile and transcription factors produced by T cells subsets in early and late-onset preeclampsia
    Authors: VR Ribeiro, M Romao-Veig, GG Romagnoli, ML Matias, PR Nunes, VTM Borges, JC Peracoli, MTS Peracoli
    Immunology, 2017-06-19;0(0):.
    Species: Human
    Sample Types: Plasma
  106. Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer
    Authors: K Gonda, M Shibata, T Ohtake, Y Matsumoto, K Tachibana, N Abe, H Ohto, K Sakurai, S Takenoshit
    Oncol Lett, 2017-06-02;14(2):1766-1774.
    Species: Human
    Sample Types: Cell Culture Supernates
  107. Frequency and role of NKp46 and NKG2A in hepatitis B virus infection
    Authors: T Yoshioka, T Tatsumi, T Miyagi, K Mukai, K Nishio, A Nishio, Y Yokoyama, T Suda, T Kegasawa, M Shigekawa, H Hikita, R Sakamori, T Takehara
    PLoS ONE, 2017-03-22;12(3):e0174103.
    Species: Human
    Sample Types: Cell Culture Supernates
  108. Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome
    Authors: KC Clark, FA Fierro, EM Ko, NJ Walker, B Arzi, CG Tepper, H Dahlenburg, A Cicchetto, A Kol, L Marsh, WJ Murphy, N Fazel, DL Borjesson
    Stem Cell Res Ther, 2017-03-20;8(1):69.
    Species: Human
    Sample Types: Cell Culture Supernates
  109. Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells
    Authors: J Liu, G Song, X Lin, X Pang, T Meng
    Exp Ther Med, 2017-02-21;13(4):1447-1455.
    Species: Human
    Sample Types: Cell Culture Supernates
  110. Development of screen-printed tryptophan-kynurenine immunosensor for in vitro assay of kynurenine-mediated immunosuppression effect of cancer cells on activated T-cells
    Authors: Yadollah Omidi
    Biosens Bioelectron, 2016-11-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  111. Therapeutic CD94/NKG2A blockade improves natural killer cell dysfunction in chronic lymphocytic leukemia
    Authors: Farrukh T Awan
    Oncoimmunology, 2016-09-09;5(10):e1226720.
    Species: Human
    Sample Types: Cell Culture Supernates
  112. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
    J Immunol, 2016-08-10;197(5):1621-30.
    Species: Human
    Sample Types: Serum
  113. Alterations in the Fecal Microbiota of Patients with HIV-1 Infection: An Observational Study in A Chinese Population
    Sci Rep, 2016-08-01;6(0):30673.
    Species: Human
    Sample Types: Serum
  114. CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma.
    Authors: Li W, Liu H
    Scand J Immunol, 2016-01-01;83(1):33-7.
    Species: Human
    Sample Types: Tissue Homogenates
  115. WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features.
    Authors: Zhao D, Xu A, Dai Z, Peng J, Zhu M, Shen J, Zheng Q, Ran Z
    BMC Gastroenterol, 2015-12-10;15(0):173.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  116. A Multidrug-resistant Engineered CAR T Cell for Allogeneic Combination Immunotherapy.
    Authors: Valton J, Guyot V, Marechal A, Filhol J, Juillerat A, Duclert A, Duchateau P, Poirot L
    Mol Ther, 2015-06-10;23(9):1507-18.
    Species: Human
    Sample Types: Cell Culture Supernates
  117. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism.
    Authors: Mankad D, Dupuis A, Smile S, Roberts W, Brian J, Lui T, Genore L, Zaghloul D, Iaboni A, Marcon P, Anagnostou E
    Mol Autism, 2015-03-21;6(0):18.
    Species: Human
    Sample Types: Plasma
  118. Comparative Multi-Donor Study of IFNgamma Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.
    Authors: Hagen J, Zimmerman R, Goetz C, Bonnevier J, Houchins J, Reagan K, Kalyuzhny A
    Cells, 2015-02-11;4(1):84-95.
    Species: Human
    Sample Types: Whole Cells
  119. Exogenous IFN-beta regulates the RANKL-c-Fos-IFN-beta signaling pathway in the collagen antibody-induced arthritis model.
    Authors: Zhao R, Chen N, Zhou X, Miao P, Hu C, Qian L, Yu Q, Zhang J, Nie H, Chen X, Li P, Xu R, Xiao L, Zhang X, Liu J, Zhang D
    J Transl Med, 2014-12-10;12(1):330.
    Species: Human
    Sample Types: Serum
  120. Psychological stress exerts effects on pathogenesis of hepatitis B via type-1/type-2 cytokines shift toward type-2 cytokine response.
    Authors: He Y, Gao H, Li X, Zhao Y
    PLoS ONE, 2014-08-21;9(8):e105530.
    Species: Human
    Sample Types: Plasma
  121. Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma.
    Authors: Han L, Liu F, Li R, Li Z, Chen X, Zhou Z, Zhang X, Hu T, Zhang Y, Young K, Sun S, Wen J, Zhang M
    Oncol Lett, 2014-07-17;8(4):1461-1469.
    Species: Human
    Sample Types: Serum
  122. Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses.
    Authors: Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, Locht C, Ausiello C
    PLoS ONE, 2014-06-26;9(6):e100166.
    Species: Human
    Sample Types: Cell Culture Supernates
  123. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
    Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
    PLoS ONE, 2014-06-16;9(6):e99612.
    Species: Human
    Sample Types: Cell Culture Supernates
  124. EBV-encoded miR-BART20-5p and miR-BART8 inhibit the IFN-gamma-STAT1 pathway associated with disease progression in nasal NK-cell lymphoma.
    Authors: Huang W, Lin C
    Am J Pathol, 2014-04-01;184(4):1185-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  125. Absence of a role for interleukin-13 in inflammatory bowel disease.
    Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
    Eur J Immunol, 2014-02-01;44(2):370-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  126. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014-01-28;9(1):e86466.
    Species: Human
    Sample Types: Cell Culture Supernates
  127. Endocannabinoids affect innate immunity of Muller glia during HIV-1 Tat cytotoxicity.
    Authors: Krishnan G, Chatterjee N
    Mol Cell Neurosci, 2014-01-11;59(0):10-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  128. Neutrophil microparticles modulate cytokine production by natural killer cells.
    Authors: Pliyev B, Kalintseva M, Abdulaeva S, Yarygin K, Savchenko V
    Cytokine, 2013-12-16;65(2):126-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  129. Effects of interval and continuous exercise training on CD4 lymphocyte apoptotic and autophagic responses to hypoxic stress in sedentary men.
    Authors: Weng T, Huang S, Chuang Y, Wang J
    PLoS ONE, 2013-11-13;8(11):e80248.
    Species: Human
    Sample Types: Plasma
  130. N6-isopentenyladenosine, an endogenous isoprenoid end product, directly affects cytotoxic and regulatory functions of human NK cells through FDPS modulation.
    Authors: Ciaglia E, Pisanti S, Picardi P, Laezza C, Malfitano A, D'Alessandro A, Gazzerro P, Vitale M, Carbone E, Bifulco M
    J Leukoc Biol, 2013-07-11;94(6):1207-19.
    Species: Human
    Sample Types: Cell Culture Supernates
  131. Multiple sclerosis lymphocytes upregulate A2A adenosine receptors that are antiinflammatory when stimulated.
    Authors: Vincenzi F, Corciulo C, Targa M, Merighi S, Gessi S, Casetta I, Gentile M, Granieri E, Borea P, Varani K
    Eur J Immunol, 2013-06-03;43(8):2206-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  132. Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis.
    Authors: Johnston A, Xing X, Swindell W, Kochkodan J, Riblett M, Nair R, Stuart P, Ding J, Voorhees J, Elder J, Gudjonsson J
    Hum Mol Genet, 2013-01-31;22(9):1807-15.
    Species: Human
    Sample Types: Serum
  133. Cleaved/associated TLR3 represents the primary form of the signaling receptor.
    Authors: Toscano F, Estornes Y, Virard F, Garcia-Cattaneo A, Pierrot A, Vanbervliet B, Bonnin M, Ciancanelli M, Zhang S, Funami K, Seya T, Matsumoto M, Pin J, Casanova J, Renno T, Lebecque S
    J Immunol, 2012-12-19;190(2):764-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  134. Analysis of the Interferon Gamma (rs2430561, +874T/A) Functional Gene Variant in Relation to the Presence of Cardiovascular Events in Rheumatoid Arthritis.
    Authors: Garcia-Bermudez M, Lopez-Mejias R, Gonzalez-Juanatey C, Corrales A, Robledo G, Castaneda S, Miranda-Filloy J, Blanco R, Fernandez-Gutierrez B, Balsa A, Gonzalez-Alvaro I, Gomez-Vaquero C, Llorca J, Martin J, Gonzalez-Gay M
    PLoS ONE, 2012-10-15;7(10):e47166.
    Species: Human
    Sample Types: Serum
  135. Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis.
    Authors: Kuriyama T, Takenaka K, Kohno K, Yamauchi T, Daitoku S, Yoshimoto G, Kikushige Y, Kishimoto J, Abe Y, Harada N, Miyamoto T, Iwasaki H, Teshima T, Akashi K
    Blood, 2012-09-18;120(19):4058-67.
    Species: Human
    Sample Types: Serum
  136. Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
    Authors: Churchman SM, Geiler J, Parmar R, Horner EA, Church LD, Emery P, Buch MH, McDermott MF, Ponchel F
    Clin. Exp. Rheumatol., 2012-08-29;30(4):534-42.
    Species: Human
    Sample Types: Serum
  137. Potent adjuvanticity of a pure TLR7-agonistic imidazoquinoline dendrimer.
    Authors: Shukla N, Salunke D, Balakrishna R, Mutz C, Malladi S, David S
    PLoS ONE, 2012-08-28;7(8):e43612.
    Species: Human
    Sample Types: Cell Culture Supernates
  138. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011-10-21;6(10):e26780.
    Species: Human
    Sample Types: Cell Culture Supernates
  139. Cytokine Response Patterns in Severe Pandemic 2009 H1N1 and Seasonal Influenza among Hospitalized Adults.
    Authors: Lee N, Wong CK, Chan PK, Chan MC, Wong RY, Lun SW, Ngai KL, Lui GC, Wong BC, Lee SK, Choi KW, Hui DS
    PLoS ONE, 2011-10-13;6(10):e26050.
    Species: Human
    Sample Types: Plasma
  140. Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
    Authors: Sun J, Fu F, Gu W, Yan R, Zhang G, Shen Z, Zhou Y, Wang H, Shen B, Zhang X
    PLoS ONE, 2011-09-13;6(9):e24751.
    Species: Human
    Sample Types: Cell Culture Supernates
  141. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
    Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
    Arthritis Res. Ther., 2011-03-24;13(2):R52.
    Species: Human
    Sample Types: Serum
  142. Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis.
    Authors: Mulder DJ, Pooni A, Mak N, Hurlbut DJ, Basta S, Justinich CJ
    Am. J. Pathol., 2011-02-01;178(2):744-53.
    Species: Human
    Sample Types: Whole Tissue
  143. Subnormal cytokine profile in the tear fluid of keratoconus patients.
    Authors: Jun AS, Cope L, Speck C, Feng X, Lee S, Meng H, Hamad A, Chakravarti S
    PLoS ONE, 2011-01-27;6(1):e16437.
    Species: Human
    Sample Types: Tears
  144. Myeloid-derived suppressor cells in the peripheral blood of cancer patients contain a subset of immature neutrophils with impaired migratory properties.
    Authors: Brandau S, Trellakis S, Bruderek K, Schmaltz D, Steller G, Elian M, Suttmann H, Schenck M, Welling J, Zabel P, Lang S
    J. Leukoc. Biol., 2010-11-24;89(2):311-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  145. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
    Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
    Leukemia, 2010-11-19;25(1):161-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  146. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
    Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
    J. Allergy Clin. Immunol., 2010-11-01;126(5):962.
    Species: Human
    Sample Types: Tissue Homogenates
  147. Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis.
    Authors: Antonelli A, Ferri C, Ferrari SM, De Marco S, Di Domenicantonio A, Centanni M, Pupilli C, Villa E, Menichetti F, Fallahi P
    J. Interferon Cytokine Res., 2010-10-07;30(11):835-42.
    Species: Human
    Sample Types: Serum
  148. Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
    Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
    J. Invest. Dermatol., 2010-08-19;131(1):133-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  149. B7-h3 augments the inflammatory response and is associated with human sepsis.
    Authors: Zhang G, Wang J, Kelly J
    J. Immunol., 2010-08-09;185(6):3677-84.
    Species: Human
    Sample Types: Plasma
  150. Shaping of iNKT cell repertoire after unrelated cord blood transplantation.
    Authors: Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debre P, Rio B, Vieillard V, Lubrano-Berthelier C, Alatrakchi N
    Clin. Immunol., 2010-02-13;135(3):364-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  151. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
    Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
    Diabetes, 2010-01-27;59(4):829-35.
    Species: Human
    Sample Types: Plasma
  152. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
    Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
    J. Immunol., 2009-12-21;184(3):1642-52.
    Species: Human
    Sample Types: Serum
  153. A novel immunomodulatory mechanism of ribavirin in suppressing natural killer cell function.
    Authors: Ogbomo H, Michaelis M, Altenbrandt B, Doerr HW, Cinatl J
    Biochem. Pharmacol., 2009-08-07;79(2):188-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  154. Cytokine secretion by human mesenchymal stem cells cocultured with damaged corneal epithelial cells.
    Authors: Oh JY, Kim MK, Shin MS, Wee WR, Lee JH
    Cytokine, 2009-02-14;46(1):100-3.
    Species: Human
    Sample Types: Cell Culture Supernates
  155. Arginase I-producing myeloid-derived suppressor cells in renal cell carcinoma are a subpopulation of activated granulocytes.
    Authors: Rodriguez PC, Ernstoff MS, Hernandez C, Atkins M, Zabaleta J, Sierra R, Ochoa AC
    Cancer Res., 2009-02-05;69(4):1553-60.
    Species: Human
    Sample Types: Plasma
  156. Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
    Authors: Mabilleau G, Sabokbar A
    PLoS ONE, 2009-01-12;4(1):e4173.
    Species: Human
    Sample Types: Cell Culture Supernates
  157. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4.
    Species: Human
    Sample Types: Cell Culture Supernates
  158. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008-12-01;181(11):8053-67.
    Species: Human
    Sample Types: BALF
  159. Staphylococcus aureus in nasal lavage and biopsy of patients with chronic rhinosinusitis.
    Authors: Niederfuhr A, Kirsche H, Deutschle T, Poppert S, Riechelmann H, Wellinghausen N
    Allergy, 2008-10-01;63(10):1359-67.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  160. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
    Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
    Int. Immunol., 2008-08-13;20(10):1321-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  161. An evaluation of commercial fluorescent bead-based luminex cytokine assays.
    Authors: Djoba Siawaya JF, Roberts T, Babb C, Black G, Golakai HJ, Stanley K, Bapela NB, Hoal E, Parida S, van Helden P, Walzl G
    PLoS ONE, 2008-07-02;3(7):e2535.
    Species: Human
    Sample Types: Cell Culture Supernates
  162. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008-04-01;38(4):1012-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  163. Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis.
    Authors: Stefanescu R, Bassett D, Modarresi R, Santiago F, Fakruddin M, Laurence J
    Blood, 2008-03-13;112(2):340-9.
    Species: Human
    Sample Types: Plasma
  164. CD200 is induced by ERK and is a potential therapeutic target in melanoma.
    Authors: Petermann KB, Rozenberg GI, Zedek D, Groben P, McKinnon K, Buehler C, Kim WY, Shields JM, Penland S, Bear JE, Thomas NE, Serody JS, Sharpless NE
    J. Clin. Invest., 2007-12-01;117(12):3922-3929.
    Species: Human
    Sample Types: Cell Culture Supernates
  165. Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke.
    Authors: Mian MF, Lauzon NM, Stampfli MR, Mossman KL, Ashkar AA
    J. Leukoc. Biol., 2007-11-30;83(3):774-84.
    Species: Human
    Sample Types: Cell Culture Supernates
  166. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
    Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
    Cytokine, 2007-10-29;40(2):128-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  167. Phase 2 study of pegylated liposomal doxorubicin in combination with interleukin-12 for AIDS-related Kaposi sarcoma.
    Authors: Little RF, Aleman K, Kumar P, Wyvill KM, Pluda JM, Read-Connole E, Wang V, Pittaluga S, Catanzaro AT, Steinberg SM, Yarchoan R
    Blood, 2007-09-10;110(13):4165-71.
    Species: Human
    Sample Types: Serum
  168. Translocation of bacterial DNA from Gram-positive microorganisms is associated with a species-specific inflammatory response in serum and ascitic fluid of patients with cirrhosis.
    Authors: Frances R, Gonzalez-Navajas JM, Zapater P, Munoz C, Cano R, Pascual S, Santana F, Marquez D, Perez-Mateo M, Such J
    Clin. Exp. Immunol., 2007-09-05;150(2):230-7.
    Species: Human
    Sample Types: Serum
  169. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007-08-30;82(6):1473-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  170. Neuroinvasion of fluorescein-positive monocytes in acute simian immunodeficiency virus infection.
    Authors: Clay CC, Rodrigues DS, Ho YS, Fallert BA, Janatpour K, Reinhart TA, Esser U
    J. Virol., 2007-08-22;81(21):12040-8.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  171. Panel of markers can accurately predict endometriosis in a subset of patients.
    Authors: Seeber B, Sammel MD, Fan X, Gerton GL, Shaunik A, Chittams J, Barnhart KT
    Fertil. Steril., 2007-08-13;89(5):1073-81.
    Species: Human
    Sample Types: Serum
  172. Fucoidan ingestion increases the expression of CXCR4 on human CD34+ cells.
    Authors: Irhimeh MR, Fitton JH, Lowenthal RM
    Exp. Hematol., 2007-06-01;35(6):989-94.
    Species: Human
    Sample Types: Plasma
  173. IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease.
    Authors: Wolk K, Witte E, Hoffmann U, Doecke WD, Endesfelder S, Asadullah K, Sterry W, Volk HD, Wittig BM, Sabat R
    J. Immunol., 2007-05-01;178(9):5973-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  174. Interferon-beta and adhesion molecules (E-selectin and s-intracellular adhesion molecule-1) are detected in sera from patients with retinal vasculitis and are induced in retinal vascular endothelial cells by Toll-like receptor 3 signalling.
    Authors: Lee MT, Hooper LC, Kump L, Hayashi K, Nussenblatt R, Hooks JJ, Detrick B
    Clin. Exp. Immunol., 2007-01-01;147(1):71-80.
    Species: Human
    Sample Types: Serum
  175. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Cell Culture Supernates
  176. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006-07-13;56(2):243-52.
    Species: Human
    Sample Types: Cell Culture Supernates
  177. Over-expression of p53 mutants in LNCaP cells alters tumor growth and angiogenesis in vivo.
    Authors: Perryman LA, Blair JM, Kingsley EA, Szymanska B, Ow KT, Wen VW, MacKenzie KL, Vermeulen PB, Jackson P, Russell PJ
    Biochem. Biophys. Res. Commun., 2006-05-11;345(3):1207-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  178. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis.
    Authors: Wolk K, Witte E, Wallace E, Docke WD, Kunz S, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2006-05-01;36(5):1309-23.
    Species: Human
    Sample Types: Plasma
  179. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
    Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
    Blood, 2006-04-13;108(3):947-55.
    Species: Human
    Sample Types: Cell Culture Supernates
  180. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
    Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
    Am. J. Respir. Crit. Care Med., 2006-03-30;174(1):51-7.
    Species: Human
    Sample Types: Plasma
  181. Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma.
    Authors: Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M
    J. Neuroimmunol., 2006-03-29;175(1):169-75.
    Species: Human
    Sample Types: Serum
  182. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
    Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
    Am. J. Respir. Crit. Care Med., 2006-03-16;173(12):1370-6.
    Species: Human
    Sample Types: Serum
  183. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Kiryushko D, Pavon L, Novitskaya V, Sandoval-Lopez G, Soroka V, Eugenia Hernandez M, Klingelhofer J, Loria F, Lukanidin E, Estrada I, Berezin V, Perez M, Bock E, Moreno J, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006-02-02;172(1):156-65.
    Species: Human
    Sample Types: Serum
  184. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006-01-15;12(2):376-82.
    Species: Human
    Sample Types: Plasma
  185. Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL).
    Authors: Hanabuchi S, Watanabe N, Wang YH, Ito T, Shaw J, Cao W, Qin FX, Liu YJ
    Blood, 2006-01-05;107(9):3617-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  186. A novel bispecific protein (ULBP2-BB4) targeting the NKG2D receptor on natural killer (NK) cells and CD138 activates NK cells and has potent antitumor activity against human multiple myeloma in vitro and in vivo.
    Authors: von Strandmann EP, Hansen HP, Reiners KS, Schnell R, Borchmann P, Merkert S, Simhadri VR, Draube A, Reiser M, Purr I, Hallek M, Engert A
    Blood, 2005-10-06;107(5):1955-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  187. Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response.
    Authors: Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I
    J. Allergy Clin. Immunol., 2005-10-01;116(4):805-11.
    Species: Human
    Sample Types: Tissue Secretion
  188. Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
    Authors: Takei M, Umeyama A, Arihara S, Matsumoto H
    J Pharm Sci, 2005-05-01;94(5):974-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  189. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005-02-24;7(3):427-36.
    Species: Human
    Sample Types: Serum
  190. Detection of interleukin 10 in cerebrospinal fluid of patients with subacute sclerosing panencephalitis.
    Authors: Mistchenko AS, Fornari MC, Viegas M, Barrero PR, Diez RA
    J. Neurovirol., 2005-02-01;11(1):66-9.
    Species: Human
    Sample Types: CSF
  191. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
    Authors: Jensen J, Krakauer M, Sellebjerg F
    Cytokine, 2005-01-07;29(1):24-30.
    Species: Human
    Sample Types: Plasma
  192. Specific recognition and killing of glioblastoma multiforme by interleukin 13-zetakine redirected cytolytic T cells.
    Authors: Kahlon KS, Brown C, Cooper LJ, Raubitschek A, Forman SJ, Jensen MC
    Cancer Res., 2004-12-15;64(24):9160-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  193. Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients.
    Authors: Saeki S, Matsuse H, Kondo Y, Machida I, Kawano T, Tomari S, Obase Y, Fukushima C, Kohno S
    J. Allergy Clin. Immunol., 2004-09-01;114(3):538-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  194. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004-08-01;21(2):241-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  195. Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors.
    Authors: Alter P, Maisch B
    Z Kardiol, 2004-07-01;93(7):524-32.
    Species: Human
    Sample Types: Serum
  196. A novel form of complete IL-12/IL-23 receptor beta1 deficiency with cell surface-expressed nonfunctional receptors.
    Authors: Fieschi C, Bosticardo M, de Beaucoudrey L, Boisson-Dupuis S, Feinberg J, Santos OF, Bustamante J, Levy J, Candotti F, Casanova JL
    Blood, 2004-06-03;104(7):2095-101.
    Species: Human
    Sample Types: Cell Culture Supernates
  197. Production of interferon gamma in respiratory syncytial virus infection of humans is not associated with interleukins 12 and 18.
    Authors: Garofalo RP, Hintz KH, Hill V, Ogra PL, Welliver RC
    J. Med. Virol., 2004-06-01;73(2):289-94.
    Species: Human
    Sample Types: Tissue Secretion
  198. Cytokines evaluation in nasal lavage of allergic children after Bacillus clausii administration: a pilot study.
    Authors: Ciprandi G, Tosca MA, Milanese M, Caligo G, Ricca V
    Pediatr Allergy Immunol, 2004-04-01;15(2):148-51.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  199. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
    Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
    J. Androl., 2004-03-01;25(2):250-4.
    Species: Human
    Sample Types: Seminal Plasma
  200. In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment.
    Authors: Durali D, de Goer de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y
    Blood, 2003-07-31;102(12):4084-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  201. IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
    Authors: Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T
    J. Immunol., 2003-06-01;170(11):5571-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  202. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003-05-01;73(5):584-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  203. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
    Authors: D&amp;apos;Ambrosio D
    Eur. J. Immunol., 2002-10-01;32(10):2934-43.
    Species: Human
    Sample Types: Cell Culture Supernates
  204. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
    Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
    Int. J. Cancer, 2002-09-10;101(2):183-9.
    Species: Human
    Sample Types: Ascites Fluid
  205. Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells.
    Authors: Lazetic S, Leong SR, Chang JC, Ong R, Dawes G, Punnonen J
    J. Biol. Chem., 2002-08-06;277(41):38660-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  206. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R.
    Authors: Parham C, Chirica M, Timans J, Vaisberg E, Travis M, Cheung J, Pflanz S, Zhang R, Singh KP, Vega F, To W, Wagner J, O&amp;apos;Farrell AM, McClanahan T, Zurawski S, Hannum C, Gorman D, Rennick DM, Kastelein RA, de Waal Malefyt R, Moore KW
    J. Immunol., 2002-06-01;168(11):5699-708.
    Species: Human
    Sample Types: Cell Culture Supernates
  207. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
    Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
    J. Immunol., 2002-05-01;168(9):4326-32.
    Species: Human
    Sample Types: Cell Culture Supernates
  208. The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx.
    Authors: Chen CD, Yang JH, Lin KC, Chao KH, Ho HN, Yang YS
    Hum. Reprod., 2002-01-01;17(1):128-33.
    Species: Human
    Sample Types: Hydrosalpinx Fluid
  209. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
    Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
    Scand. J. Rheumatol., 2002-01-01;31(4):205-10.
    Species: Human
    Sample Types: Serum
  210. Cytokine pattern in blister fluid and sera of patients with pemphigus.
    Authors: Baroni A, Perfetto B, Ruocco E, Greco R, Criscuolo D, Ruocco V
    Dermatology (Basel), 2002-01-01;205(2):116-21.
    Species: Human
    Sample Types: Blister Fluid
  211. An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon.
    Authors: Cotler SJ, Reddy KR, McCone J, Wolfe DL, Liu A, Craft TR, Ferris MW, Conrad AJ, Albrecht J, Morrissey M, Ganger DR, Rosenblate H, Blatt LM, Jensen DM, Taylor MW
    J. Interferon Cytokine Res., 2001-12-01;21(12):1011-9.
    Species: Human
    Sample Types: Serum
  212. Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies.
    Authors: Jotwani R, 175, Palucka AK, Al-Quotub M, Nouri-Shirazi M, Kim J, Bell D, Banchereau J, Cutler CW
    2001-10-15;167(8):4693-700.
    Species: Human
    Sample Types:
  213. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001-10-01;70(4):617-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  214. Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses.
    Authors: Rigano R, Profumo E, Bruschi F, Carulli G, Azzara A, Ioppolo S, Buttari B, Ortona E, Margutti P, Teggi A, Siracusano A
    Infect. Immun., 2001-01-01;69(1):288-96.
    Species: Human
    Sample Types: Cell Culture Supernates
  215. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000-07-17;192(2):219-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  216. Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity.
    Authors: Black, K P, Merrill, K W, Jackson, S, Katz, J
    Oral Microbiol Immunol, 2000-04-01;15(2):74-81.
    Species: Human
    Sample Types: Saliva
  217. Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis.
    Authors: Benson M, Strannegard IL, Wennergren G, Strannegard O
    Pediatr Allergy Immunol, 2000-02-01;11(1):20-8.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  218. Restoration of cytotoxic T lymphocyte function in malignant pleural effusion: interleukin-15 vs. interleukin-2.
    Authors: Chen YM, Ting CC, Peng JW, Yang WK, Yang KY, Tsai CM, Perng RP
    J. Interferon Cytokine Res., 2000-01-01;20(1):31-9.
    Species: Human
    Sample Types: Pleural Effusion
  219. Cytokine production in rhesus monkeys infected with Plasmodium coatneyi.
    Authors: Yang C, Xiao L, Tongren JE, Sullivan J, Lal AA, Collins WE
    Am. J. Trop. Med. Hyg., 1999-08-01;61(2):226-9.
    Species: Primate
    Sample Types: Serum

FAQs

  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Reviews for Human IFN-gamma Quantikine ELISA Kit

Average Rating: 4.8 (Based on 10 Reviews)

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Human IFN-gamma Quantikine ELISA Kit
By Anonymous on 02/27/2023
Sample Tested: Cell culture supernatant
Human IFN-gamma Quantikine ELISA Kit
By Anonymous on 11/16/2022
Sample Tested: Plasma

Plasma tested at 2-fold dilution. Strong matrix effect observed for S6 & S7.

Human IFN-gamma Quantikine ELISA Kit DIF50C
Human IFN-gamma Quantikine ELISA Kit
By Lu Bai on 12/22/2021
Sample Tested: Cell culture supernatant,human PBMC
Human IFN-gamma Quantikine ELISA Kit
By Anonymous on 09/15/2021
Sample Tested: human primary NK cells,Peripheral blood mononuclear cells (PBMCs)

PBMCs from healthy donors or isolated primary human NK cells were stimulated and tested for IFNg secretion. Following protocol, it was easy to obtain clean and consistent results between indpendent experiments.

Human IFN-gamma Quantikine ELISA Kit DIF50C
Human IFN-gamma Quantikine ELISA Kit
By Anonymous on 03/11/2021
Sample Tested: HepG2 human hepatocellular carcinoma cell line,MDA-MB-231 human breast cancer cell line

Easy to use, protocol detailed and easy to follow.
Reproducible standard curve.

Human IFN-gamma Quantikine ELISA Kit DIF50C
Human IFN-gamma Quantikine ELISA Kit
By Anonymous on 02/24/2021
Sample Tested: Cancer Cells,T cells
Human IFN-gamma Quantikine ELISA Kit DIF50C
Human IFN-gamma Quantikine ELISA Kit
By Anonymous on 01/22/2021
Sample Tested: HepG2 human hepatocellular carcinoma cell line,MDA-MB-231 human breast cancer cell line

The assay was easy to perform according to the supplied insctructions. We were satisfied with the standard curve, for the samples the right dilution still needs to be figured out.

Human IFN-gamma Quantikine ELISA Kit
By Anonymous on 11/13/2020
Sample Tested: Serum

Standards in duplicate in all the wells in 1 and 2.
R&D QC Low in well 3A
R&D QC Med in well 12G
R&D QC High in well 12H

Pipetting error or washing error didnt develop the best standard curve however the rest worked great.

Human IFN-gamma Quantikine ELISA Kit DIF50C
Human IFN-gamma Quantikine ELISA Kit
By Lindsie Morin on 11/12/2020
Sample Tested: Serum

Great protocol that was easy to follow. The curve developed very well with expected results.

Human IFN-gamma Quantikine ELISA Kit DIF50C
Human IFN-gamma Quantikine ELISA Kit
By Anonymous on 11/12/2020
Sample Tested: EDTA Plasma

Excellent background detail that was relevant for understanding the assay, and easy to follow protocol.

Human IFN-gamma Quantikine ELISA Kit DIF50C