Human IFN-gamma R1/CD119 Antibody

Catalog #: MAB6732
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6732-SP
MAB6732-100
MAB6732-500

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody.

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Citations (5)

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Human IFN-gamma R1/CD119 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN‑ gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta is observed.

Source

Monoclonal Mouse IgG₁ Clone # GIR208

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human placenta-derived IFN‑ gamma R1/CD119

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize IFN‑ gamma R1/CD119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.03-0.15 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN-gamma R1/CD119 Mono-clonal Antibody (Catalog # MAB6732). The ND₅₀ is typically 0.03-0.15 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% aa identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.

References

Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Long Name

Interferon gamma Receptor 1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Alternate Names

AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2

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Citations for Human IFN-gamma R1/CD119 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Mass cytometry identifies a distinct monocyte cytokine signature shared by clinically heterogeneous pediatric SLE patients
Authors: William E. O'Gorman, Daniel S. Kong, Imelda M. Balboni, Pratryaydipta Rudra, Christopher R. Bolen, Debashish Ghosh et al.
Journal of Autoimmunity
Bispecific T cell engager (BiTEï¿½) antibody constructs can mediate bystander tumor cell killing
Authors: SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson
PLoS ONE, 2017-08-24;12(8):e0183390.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Chronic lymphocytic leukemia cells express CD38 in response to Th1 cell-derived IFN-gamma by a T-bet-dependent mechanism.
Authors: Burgler S, Gimeno A, Parente-Ribes A, Wang D, Os A, Devereux S, Jebsen P, Bogen B, Tjonnfjord G, Munthe L
J Immunol, 2014-12-10;194(2):827-35.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients.
Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S
J Allergy Clin Immunol, 2012-11-19;131(2):562-70.
Species: Human
Sample Types: Whole Cells
Applications: Blocking
A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
J. Leukoc. Biol., 2001-10-01;70(4):617-23.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization