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Human IGFBP-6 Antibody

Catalog #: MAB8761
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8761
MAB8761-SP
Product Details
Citations (5)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGFBP-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP-6 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) IGFBP-1, -2, -3, -4, -5, rmIGFBP-3, -5, or -6.
Source
Monoclonal Mouse IgG2B Clone # 110211
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP-6
Ala25-Gly240
Accession # P24592
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IGFBP‑6 (Catalog # 876-B6)

Human IGFBP-6 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IGFBP‑6 Biotinylated Antibody (Catalog # BAF876)

Standard: Recombinant Human IGFBP-6 Protein, CF (Catalog # 876-B6)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-6

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-6 cDNA encodes a 240 amino acid (aa) residue precursor protein with a putative 24 aa residue signal peptide that is processed to generate the 216 aa residue mature protein that is O-glycosylated. IGFBP-6 is expressed in ovarian cells, prostatic cells, and fibroblasts. IGFBP-6 is found predominantly in CSF and serum. IGFBP-6 binds preferentially to IGF-II, exhibiting a 2-fold higher affinity for IGF-II than for IGF-I.

References
  1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Long Name
Insulin-like Growth Factor Binding Protein 6
Entrez Gene IDs
3489 (Human); 16012 (Mouse)
Alternate Names
IBP6; IBP-6; IGF binding protein 6; IGF-binding protein 6; IGFBP6; IGFBP-6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6

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Citations for Human IGFBP-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes
    Authors: M Conese, L Pace, N Pignataro, L Catucci, A Ambrosi, S Di Gioia, N Tartaglia, A Liso
    Int J Mol Sci, 2020-06-22;21(12):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker
    Authors: Q Chen, S Qin, Y Liu, M Hong, CN Qian, ET Keller, J Zhang, Y Lu
    Oncotarget, 2016-10-18;7(42):68140-68150.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  3. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  4. Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
    Authors: Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A
    Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  5. Purification and characterization of native human insulin-like growth factor binding protein-6
    Authors: Andrea Taferner, Lucia Micutkova, Martin Hermann, Pidder Jansen-Dürr, Haymo Pircher
    Journal of Cell Communication and Signaling

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