Human IgG APC-conjugated Antibody

Name: Human IgG APC-conjugated Antibody
Brand: R&D Systems
SKU: F0135
Rating: 5 (1 reviews)

Catalog #: F0135
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
F0135

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.

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All IgG Antibodies

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Human IgG APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

This antibody reacts with heavy chains on human IgG and with light chains on all human immunoglobulins. No reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins by IEP.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Human IgG

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of human origin, and is of IgG class. The recommended concentration is 10 μL/10⁶cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Cetuximab) Monoclonal Antibody (Catalog # MAB9577, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog #F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

Product Datasheet

COA

SDS

Citation for Human IgG APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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