Human IgG Fc APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB110A In Stock25 Tests / $209
FAB110A-100 In Stock100 Tests / $419

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Detection of IgG1Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.
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Product Details
Citations (6)
FAQs
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Human IgG Fc APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 97924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IgG<SUB>1</SUB>Fc antibody in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of IgG1Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry. Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with either (A) Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). Panel C shows HEK293 cell line transfected with human PD-1, untreated with B7-H1 Fc Chimera, and stained with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

Human IgG Fc APC‑conjugated Antibody Monoclonal Mouse IgG1 Clone # 97924

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Human IgG Fc APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. ZL-1211 Exhibits Robust Antitumor Activity by Enhancing ADCC and Activating NK Cell–mediated Inflammation in CLDN18.2-High and -Low Expressing Gastric Cancer Models
    Authors: Hiroyasu Konno, Tracey Lin, Renyi Wu, Xinchuan Dai, Shou Li, Grace Wang et al.
    Cancer Research Communications
  2. Early Reconstitution of Antibody Secreting Cells after Allogeneic Stem Cell Transplantation
    Authors: M Hinterleit, C Hinterleit, E Malenke, B Federmann, U Holzer, M Müller, WA Bethge, S Wirths
    Journal of Clinical Medicine, 2022-01-05;11(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by�epistasis acting on ACE2
    Authors: GM Castiglion, L Zhou, Z Xu, Z Neiman, CF Hung, EJ Duh
    PloS Biology, 2021-12-21;19(12):e3001510.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species
    Authors: J Hunka, JT Riley, GF Debes
    BMC Vet. Res., 2020-03-06;16(1):83.
    Species: Sheep
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma
    Authors: HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland
    JCI Insight, 2019-07-11;4(13):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Frequency and role of NKp46 and NKG2A in hepatitis B virus infection
    Authors: T Yoshioka, T Tatsumi, T Miyagi, K Mukai, K Nishio, A Nishio, Y Yokoyama, T Suda, T Kegasawa, M Shigekawa, H Hikita, R Sakamori, T Takehara
    PLoS ONE, 2017-03-22;12(3):e0174103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. What is the light chain of the Human IgG Fc Antibody, Catalog #s MAB110 and FAB110, Clone # 97924?

    • Catalog #s MAB110 and FAB110 have a kappa light chain.

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