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Human IGSF3 Antibody

Catalog #: AF4788
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4788
AF4788-SP
Detection of IGSF3 in A549 Human Cell Line by Flow Cytometry.
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Product Details
Citations (3)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGSF3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGSF3 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGSF3
Gln20-Ala1125
Accession # AAI10652
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot

Human lung tissue under non-reducing conditions only

 
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IGSF3 antibody in A549 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IGSF3 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Sheep Anti-Human IGSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4788, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Western Blot Detection of Human IGSF3 antibody by Western Blot. View Larger

Detection of Human IGSF3 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IGSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4788) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF3

IGSF3 (Immunoglobulin superfamily member 3; also EWI-3) is a 133 kDa (predicted) member of the EWI subfamily of the Ig-Superfamily of molecules. It is reported to be widely expressed, with concentration in placenta and lung. Human IGSF3 an 1194 amino acid (aa) type I transmembrane protein that contains a 19 aa signal sequence followed by an 1175 aa mature region (aa 20-1194) (SwissProt # O75054). The molecule's extracellular region is 1105 aa in length (aa 20-1124), and contains eight C2-type Ig-like domains (aa 22-1097). This region also possesses at least two utilized phosphorylation sites at Thr617and Ser625, plus an EWI motif in the second Ig-like domain. IGSF3 likely exists as a disulfide-linked homodimer. There are two potential isoform variants. One contains a 20 aa insertion after Pro406, while another shows the same insert coupled to a premature truncation after Pro833. GenBank Accession # AAI10652 is identical to the SwissProt sequence above save for a double Glu substitution for Asp1020 in the eighth Ig-like domain. Over aa 20‑1125, human IGSF3 shares 92% aa identity with mouse IGSF3.

Long Name
Immunoglobulin Superfamily, Member 3
Entrez Gene IDs
3321 (Human); 78908 (Mouse); 295325 (Rat)
Alternate Names
EWI-3; glu-Trp-Ile EWI motif-containing protein 3; IGSF3; immunoglobulin superfamily, member 3; member 3; V8

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Citations for Human IGSF3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Photoproximity labeling of endogenous receptors in the live mouse brain in minutes
    Authors: Takato, M;Sakamoto, S;Nonaka, H;Tanimura Valor, FY;Tamura, T;Hamachi, I;
    Nature chemical biology
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: Immunohistochemistry, Western Blot
  2. Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
    Authors: Tanjore Ramanathan, J;Zárybnický, T;Filppu, P;Monzo, HJ;Monni, O;Tervonen, TA;Klefström, J;Kerosuo, L;Kuure, S;Laakkonen, P;
    Scientific reports
    Species: Human
    Sample Types: Tissue Homoegenates, Whole Tissue
    Applications: IHC, Western Blot
  3. IGSF3 mutation identified in patient with severe COPD alters cell function and motility
    Authors: KS Schweitzer, N Jinawath, R Yonescu, K Ni, N Rush, V Charoensaw, I Bronova, E Berdyshev, SM Leach, LA Gillenwate, RP Bowler, DB Pearse, CA Griffin, I Petrache
    JCI Insight, 2020-07-23;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot

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