Human IGSF4B/SynCAM3 Antibody

Catalog #: MAB3678 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3678
MAB3678-SP

Detection of Human IGSF4B/SynCAM3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGSF4B/SynCAM3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IGSF4B/SynCAM3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IGSF3, 4, 4C, 4D, recombinant mouse IGSF4, or 8 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 730004

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IGSF4B/SynCAM3
Pro21-Tyr329
Accession # Q8N126

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IGSF4B/SynCAM3 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (cortex) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human IGSF4B/SynCAM3 Monoclonal Antibody (Catalog # MAB3678) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGSF4B/SynCAM3 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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IGSF4B/SynCAM3 in Human Brain. IGSF4B/SynCAM3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human IGSF4B/SynCAM3 Monoclonal Antibody (Catalog # MAB3678) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF4B/SynCAM3

IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl‑1 and SynCAM-3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48‑50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 398 amino acid (aa) human IGSF4B contains a 24 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca⁺⁺-independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl‑2 (IGSF-4) in trans. It also contains two C2-type Ig-like domains that are responsible for Ca⁺⁺-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). Of two known splice variants, one shows an insertion of 34 aa near the mature N-terminus and probably represents a 60 kDa form. A smaller variant shows deletions of aa 185‑231, and 281‑398, which includes the first C2-type Ig-like domain and the TM segment. The IGSF4B ECD is highly conserved, sharing 95‑96% aa identity between human mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35‑50% aa identity.

References

Fukuhara, H. et al. (2001) Oncogene 20:5401.
Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.
Fukami, T. et al. (2003) Gene 323:11.
Shingai, T. et al. (2003) J. Biol. Chem. 278:35421
Dong, X. et al. (2006) J. Biol. Chem. 281:10610.

Long Name

Immunoglobulin Superfamily, Member 4B

Entrez Gene IDs

57863 (Human); 94332 (Mouse); 360882 (Rat)

Alternate Names

BIgR; Brain immunoglobulin receptor; CADM3; cell adhesion molecule 3; IGSF4B; IGSF4BSynCAM3; Immunoglobulin superfamily member 4B; member 4B; Necl-1; NECL1TSLC1-like protein 1; Nectin-like protein 1; Synaptic cell adhesion molecule 3; SynCAM3; TSLC1-like 1; TSLL1; TSLL1SYNCAM3