Home / IL-1 RI / Human IL-1 RI PE-conjugated Antibody

Human IL-1 RI PE-conjugated Antibody

Catalog #: FAB269P
23 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB269P-100
FAB269P-025
Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry.
1 Image
Product Details
Citations (23)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1 RI PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 RI in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-1 RI antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human IL-1 RI PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB269P, filled histogram) or control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 RI

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

References
  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
Long Name
Interleukin 1 Receptor I
Entrez Gene IDs
3554 (Human); 16177 (Mouse)
Alternate Names
CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human IL-1 RI PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Inhibition of interleukin-1beta reduces myelofibrosis and osteosclerosis in mice with JAK2-V617F driven myeloproliferative neoplasm
    Authors: S Rai, E Grockowiak, N Hansen, D Luque Paz, CB Stoll, H Hao-Shen, G Mild-Schne, S Dirnhofer, CJ Farady, S Méndez-Fer, RC Skoda
    Nature Communications, 2022-09-13;13(1):5346.
  2. A variable-gain stochastic pooling motif mediates information transfer from receptor assemblies into NF-kappa B
    Authors: J. Agustin Cruz, Chaitanya S. Mokashi, Gabriel J. Kowalczyk, Yue Guo, Qiuhong Zhang, Sanjana Gupta et al.
    Science Advances
  3. Regulation of PD-L1 expression in non–small cell lung cancer by interleukin-1 beta
    Authors: Aiko Hirayama, Kentaro Tanaka, Hirono Tsutsumi, Takayuki Nakanishi, Sho Yamashita, Shun Mizusaki et al.
    Frontiers in Immunology
  4. Serum Amyloid A1 (SAA1) Revisited: Restricted Leukocyte-Activating Properties of Homogeneous SAA1
    Authors: Sara Abouelasrar Salama, Mirre De Bondt, Mieke De Buck, Nele Berghmans, Paul Proost, Vivian Louise Soares Oliveira et al.
    Frontiers in Immunology
  5. Kinetics of peripheral blood neutrophils in severe coronavirus disease 2019
    Authors: Mieke Metzemaekers, Seppe Cambier, Marfa Blanter, Jennifer Vandooren, Ana Carolina Carvalho, Bert Malengier‐Devlies et al.
    Clinical & Translational Immunology
  6. The human G protein‐coupled ATP receptor P2Y11 is a target for anti‐inflammatory strategies
    Authors: Georg Gruenbacher, Hubert Gander, Gabriele Dobler, Andrea Rahm, Dominik Klaver, Martin Thurnher
    British Journal of Pharmacology
  7. Relationship between interleukin-1 type 1 and 2 receptor gene polymorphisms and the expression level of membrane-bound receptors
    Authors: F F Vasilyev, A N Silkov, S V Sennikov
    Cellular & Molecular Immunology
  8. IL1R1+ cancer-associated fibroblasts drive tumor development and immunosuppression in colorectal cancer
    Authors: Koncina, E;Nurmik, M;Pozdeev, VI;Gilson, C;Tsenkova, M;Begaj, R;Stang, S;Gaigneaux, A;Weindorfer, C;Rodriguez, F;Schmoetten, M;Klein, E;Karta, J;Atanasova, VS;Grzyb, K;Ullmann, P;Halder, R;Hengstschläger, M;Graas, J;Augendre, V;Karapetyan, YE;Kerger, L;Zuegel, N;Skupin, A;Haan, S;Meiser, J;Dolznig, H;Letellier, E;
    Nature communications
    Species:  Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Human TH17 cells engage gasdermin E pores to release IL-1alpha on NLRP3 inflammasome activation
    Authors: YY Chao, A Puhach, D Frieser, M Arunkumar, L Lehner, T Seeholzer, A Garcia-Lop, M van der Wa, S Fibi-Smeta, A Dietschman, T Sommermann, T ?ikovi?, L Taher, MS Gresnigt, SJ Vastert, F van Wijk, G Panagiotou, D Krappmann, O Gro beta, CE Zielinski
    Nature Immunology, 2023-01-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Pathogenic function of bystander-activated memory-like CD4+ T cells in autoimmune encephalomyelitis
    Authors: HG Lee, JU Lee, DH Kim, S Lim, I Kang, JM Choi
    Nat Commun, 2019-02-12;10(1):709.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Regulation of radiosensitivity by 4-methylumbelliferone via the suppression of interleukin-1 in fibrosarcoma cells
    Authors: R Saga, K Hasegawa, K Murata, M Chiba, T Nakamura, K Okumura, E Tsuruga, Y Hosokawa
    Oncol Lett, 2019-01-29;17(3):3555-3561.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015-08-14;195(6):2818-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Critical role of all-trans retinoic acid in stabilizing human natural regulatory T cells under inflammatory conditions.
    Authors: Lu L, Lan Q, Li Z, Zhou X, Gu J, Li Q, Wang J, Chen M, Liu Y, Shen Y, Brand D, Ryffel B, Horwitz D, Quismorio F, Liu Z, Li B, Olsen N, Zheng S
    Proc Natl Acad Sci U S A, 2014-08-06;111(33):E3432-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Differentiation of IL-17-producing effector and regulatory human T cells from lineage-committed naive precursors.
    Authors: Mercer F, Khaitan A, Kozhaya L, Aberg J, Unutmaz D
    J Immunol, 2014-06-23;193(3):1047-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1beta and TRIF/IRF3.
    Authors: Endo Y, Blinova K, Romantseva T, Golding H, Zaitseva M
    PLoS ONE, 2014-05-28;9(5):e98517.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Flow Cytometry
  16. Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
    Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
    J Immunol, 2014-05-16;192(12):5776-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. T cell-derived IL-22 amplifies IL-1beta-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes.
    Authors: Dalmas E, Venteclef N, Caer C, Poitou C, Cremer I, Aron-Wisnewsky J, Lacroix-Desmazes S, Bayry J, Kaveri S, Clement K, Andre S, Guerre-Millo M
    Diabetes, 2014-02-11;63(6):1966-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010-10-22;185(11):7047-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Regulating human Th17 cells via differential expression of IL-1 receptor.
    Authors: Lee WW, Kang SW, Choi J
    Blood, 2009-11-12;115(3):530-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. The P2Y11 receptor of human M2 macrophages activates canonical and IL-1 receptor signaling to translate the extracellular danger signal ATP into anti-inflammatory and pro-angiogenic responses
    Authors: Dominik Klaver, Hubert Gander, Gabriele Dobler, Andrea Rahm, Martin Thurnher
    Cellular and Molecular Life Sciences
  21. Optimized flow cytometry protocol for analysis of surface expression of interleukin-1 receptor types I and II
    Authors: Filipp Filippovich Vasilyev, Julia Anatolievna Lopatnikova, Sergey Vitalievich Sennikov
    Cytotechnology
  22. Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1 beta -dependent response of Th17 cells
    Authors: Dong Hyun Kim, Hee Young Kim, Sunjung Cho, Su-Jin Yoo, Won-Ju Kim, Hye Ran Yeon et al.
    eLife
  23. Single-cell RNA sequencing identifies a population of human liver-type ILC1s
    Authors: B Krämer, AP Nalin, F Ma, S Eickhoff, P Lutz, S Leonardell, F Goeser, C Finnemann, G Hack, J Raabe, M ToVinh, S Ahmad, C Hoffmeiste, KM Kaiser, S Manekeller, V Branchi, T Bald, M Hölzel, R Hüneburg, HD Nischalke, A Semaan, B Langhans, DJ Kaczmarek, B Benner, MR Lordo, J Kowalski, A Gerhardt, J Timm, M Toma, R Mohr, A Türler, A Charpentie, T van Bremen, G Feldmann, A Sattler, K Kotsch, AT Abdallah, CP Strassburg, U Spengler, WE Carson, BL Mundy-Boss, M Pellegrini, TE O'Sullivan, AG Freud, J Nattermann
    Cell Reports, 2023-01-01;42(1):111937.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human IL-1 RI PE-conjugated Antibody

Average Rating: 4.7 (Based on 3 Reviews)

5 Star
66.67%
4 Star
33.33%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human IL-1 RI PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human IL-1 RI PE-conjugated Antibody
By Anonymous on 07/26/2020
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human
Flow Cytometry Human IL-1 RI PE-conjugated Antibody FAB269P
Human IL‑1 RI PE‑conjugated Antibody
By Anonymous on 05/02/2018
Application: Flow Sample Tested: Pancreatic cancer cells Species: Human
Human IL-1 RI PE-conjugated Antibody
By Aida Flores on 05/17/2016
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human