Human IL-10 Quantikine HS ELISA Kit Summary
Product Summary
Precision
Serum, EDTA Plasma, Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 35 | 35 | 35 |
Mean (pg/mL) | 2.36 | 10.7 | 21 | 1.68 | 10.4 | 20.9 |
Standard Deviation | 0.22 | 0.62 | 0.97 | 0.22 | 0.81 | 1.78 |
CV% | 9.4 | 5.8 | 4.6 | 12.8 | 7.8 | 8.5 |
Recovery
The recovery of IL-10 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Citrate Plasma (n=4) | 93 | 81-106 |
EDTA Plasma (n=4) | 90 | 84-99 |
Serum (n=4) | 100 | 94-108 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: IL-10
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature human IL-10 shares 86% amino acid sequence identity with equine IL-10 and 72% - 80% with bovine, canine, feline, guinea pig, mouse, ovine, porcine, and rat IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7).
Citations for Human IL-10 Quantikine HS ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Exercise4Psychosis: A randomised control trial assessing the effect of moderate-to-vigorous exercise on inflammatory biomarkers and negative symptom profiles in men with first-episode psychosis
Authors: Dunleavy, C;Elsworthy, RJ;Wood, SJ;Allott, K;Spencer, F;Upthegrove, R;Aldred, S;
Brain, behavior, and immunity
Species: Human
Sample Types: Serum, Plasma
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IL-10 and IL-1? Serum Levels, Genetic Variants, and Metabolic Syndrome: Insights into Older Adults' Clinical Characteristics
Authors: Freitas, RS;de Souza Silva, CM;Ferreira Fratelli, C;Ramos de Lima, L;Morato Stival, M;Schwerz Funghetto, S;Rodrigues da Silva, IC;Vieira de Andrade, R;
Nutrients
Species: Human
Sample Types: Serum
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The Impact of Metabolic Memory on Immune Profile in Young Patients with Uncomplicated Type 1 Diabetes
Authors: Neubauer-Geryk, J;Wielicka, M;My?liwiec, M;Zorena, K;Bieniaszewski, L;
International journal of molecular sciences
Species: Human
Sample Types: Serum
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Patterns of IgA Autoantibody Generation, Inflammatory Responses and Extracellular Matrix Metabolism in Patients with Alcohol Use Disorder
Authors: Niemelä, O;Bloigu, A;Bloigu, R;Nivukoski, U;Kultti, J;Pohjasniemi, H;
International journal of molecular sciences
Species: Human
Sample Types: Serum
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Possible Correlation between Urocortin 1 (Ucn1) and Immune Parameters in Patients with Endometriosis
Authors: Abramiuk, M;Frankowska, K;Ku?ak, K;Tarkowski, R;Mertowska, P;Mertowski, S;Grywalska, E;
International journal of molecular sciences
Species: Human
Sample Types: Serum
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Peripheral blood CD4posCD25posFoxP3pos cells and inflammatory cytokines as biomarkers of response in rheumatoid arthritis patients treated with CTLA4-Ig
Authors: G Elisa, B Tolusso, L Petricca, C Di Mario, MR Gigante, G Ferracciol, S Alivernini
Arthritis Research & Therapy, 2022-06-15;24(1):143.
Species: Human
Sample Types: Serum
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Klotho expression in peripheral blood circulating cells is associated with vascular and systemic inflammation in atherosclerotic vascular disease
Authors: E Martín-Núñ, A Pérez-Cast, VG Tagua, C Hernández-, C Ferri, N Pérez-Delg, S Rodríguez-, P Cerro-Lópe, Á López-Cast, A Delgado-Mo, VC López-Tarr, MA Arévalo-Gó, A González-L, A Martín-Oli, CC Morales-Es, C Mora-Ferná, J Donate-Cor, JF Navarro-Go
Scientific Reports, 2022-05-19;12(1):8422.
Species: Human
Sample Types: Serum
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Effects of resistance exercise and whey protein supplementation on skeletal muscle strength, mass, physical function, and hormonal and inflammatory biomarkers in healthy active older men: A randomised, double-blind, placebo-controlled trial
Authors: C Griffen, M Duncan, J Hattersley, MO Weickert, A Dallaway, D Renshaw
Experimental gerontology, 2021-12-09;0(0):111651.
Species: Human
Sample Types: Plasma
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Impaired Innate Immunity in Pediatric Patients Type 1 Diabetes-Focus on Toll-like Receptors Expression
Authors: K Kurianowic, M Klatka, A Polak, A Hymos, D B?bnowska, M Podgajna, R Hrynkiewic, O Sierawska, P Nied?wiedz
International Journal of Molecular Sciences, 2021-11-09;22(22):.
Species: Human
Sample Types: Plasma
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Association between Biomarkers of Oxidative Stress and Inflammation with Cardiac Necrosis and Heart Failure in Non-ST Segment Elevation Myocardial Infarction Patients and Various Degrees of Kidney Function
Authors: S Roumelioti, A Veljkovic, PI Georgianos, G Lazarevic, Z Perisic, J Hadzi-Djok, V Liakopoulo, G Kocic
Oxidative Medicine and Cellular Longevity, 2021-11-01;2021(0):3090120.
Species: Human
Sample Types: Serum
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Chemotherapy-Related Differences in Cognitive Functioning and Their Biological Predictors in Patients with Multiple Myeloma
Authors: M Bury-Kami?, A Szudy-Szcz, A Nowaczy?sk, O Jankowska-, M Hus, K Kot
Brain sciences, 2021-09-01;11(9):.
Species: Human
Sample Types: Serum
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Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men
Authors: M Wiecek, Z Szygula, J Gradek, J Kusmierczy, J Szymura
Biomolecules, 2021-07-16;11(7):.
Species: Human
Sample Types: Plasma
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Comparative evaluation of inflammatory parameters and substitute insulin resistance indices in elderly women with and without type 2 diabetes mellitus
Authors: MM Leite, MT Dutra, MVG da Costa, SS Funghetto, AO Silva, LR de Lima, ICR da Silva, MR Mota, MM Stival
Experimental gerontology, 2021-05-03;150(0):111389.
Species: Human
Sample Types: Plasma
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Reliability and suitability of physiological exercise response and recovery markers
Authors: T Reichel, TK Bo beta lau, J Palmowski, K Eder, R Ringseis, FC Mooren, R Walscheid, E Bothur, S Samel, T Frech, M Philippe, K Krüger
Sci Rep, 2020-07-17;10(1):11924.
Species: Human
Sample Types: Plasma
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Effects of Pre-Sleep Whey vs. Plant-Based Protein Consumption on Muscle Recovery Following Damaging Morning Exercise
Authors: PG Saracino, HE Saylor, BR Hanna, RC Hickner, JS Kim, MJ Ormsbee
Nutrients, 2020-07-10;12(7):.
Species: Human
Sample Types: Plasma
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Overexpression of PD-1 on Peripheral Blood Lymphocytes in Patients with Idiopathic Pulmonary Arterial Hypertension and Its Association with High Viral Loads of Epstein-Barr Virus and Poor Clinical Parameters
Authors: M Tomaszewsk, E Grywalska, A Tomaszewsk, P B?aszczak, M Kurzyna, J Roli?ski, G Kope?
J Clin Med, 2020-06-24;9(6):.
Species: Human
Sample Types: Plasma
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Interferon and anti-TNF therapies differentially modulate amygdala reactivity which predicts associated bidirectional changes in depressive symptoms
Authors: KA Davies, E Cooper, V Voon, J Tibble, M Cercignani, NA Harrison
Mol. Psychiatry, 2020-05-26;0(0):.
Species: Human
Sample Types: Plasma
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Association between serum levels of Klotho and inflammatory cytokines in cardiovascular disease: a case-control study
Authors: E Martín-Núñ, J Donate-Cor, C Ferri, Á López-Cast, A Delgado-Mo, C Hernández-, N Pérez-Delg, S Rodríguez-, P Cerro-Lópe, VG Tagua, C Mora-Ferná, JF Navarro-Go
Aging (Albany NY), 2020-01-27;12(2):1952-1964.
Species: Human
Sample Types: Serum
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Inflamm-Aging Is Associated with Lower Plasma PTX3 Concentrations and an Impaired Capacity of PBMCs to Express hTERT following LPS Stimulation
Authors: AL Slusher, TM Zúñiga, EO Acevedo
Mediators Inflamm., 2019-09-12;2019(0):2324193.
Species: Human
Sample Types: Plasma
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LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy
Authors: J Doll, A Moghaddam, V Daniel, B Biglari, R Heller, G Schmidmaie, TF Raven
J Orthop, 2019-08-13;17(0):208-214.
Species: Human
Sample Types: Serum
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Vascular Adhesion Protein-1 (VAP-1) as Predictor of Radiographic Severity in Symptomatic Knee Osteoarthritis in the New York University Cohort
Authors: E Bournazou, J Samuels, H Zhou, S Krasnokuts, J Patel, T Han, J Bencardino, L Rybak, SB Abramson, U Junker, KS Brown, M Attur
Int J Mol Sci, 2019-05-29;20(11):.
Species: Human
Sample Types: Synovial Fluid
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Interleukin-10 as a predictor of major adverse cardiovascular events in a racially and ethnically diverse population: Multi-Ethnic Study of Atherosclerosis
Authors: D Goldwater, A Karlamangl, SS Merkin, K Watson, T Seeman
Ann Epidemiol, 2018-09-05;0(0):.
Species: Human
Sample Types: Serum
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Pilot study of placental tissue collection, processing, and measurement procedures for large scale assessment of placental inflammation
Authors: LA Sjaarda, KA Ahrens, DL Kuhr, TL Holland, UR Omosigho, BT Steffen, NL Weir, HK Tollman, RM Silver, MY Tsai, EF Schisterma
PLoS ONE, 2018-05-11;13(5):e0197039.
Species: Human
Sample Types: Tissue Homogenates
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The ties that bind: Ingroup ties are linked with diminished inflammatory immune responses and fewer mental health symptoms through less rumination
Authors: R Ysseldyk, RJ McQuaid, OA McInnis, H Anisman, K Matheson
PLoS ONE, 2018-04-23;13(4):e0195237.
Species: Human
Sample Types: Plasma
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Heat and Hypoxic Acclimation Increase Monocyte Heat Shock Protein 72 but Do Not Attenuate Inflammation following Hypoxic Exercise
Authors: BJ Lee, CD Thake
Front Physiol, 2017-10-16;8(0):811.
Species: Human
Sample Types: Plasma
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Serum and peritoneal fluid concentrations of soluble human leukocyte antigen, tumor necrosis factor alpha and interleukin 10 in patients with selected ovarian pathologies
Authors: O Sipak-Szmi, P W?odarski, E Ronin-Walk, A Niedzielsk, B Karakiewic, S S?uczanows, M Laszczy?sk, W Malinowski
J Ovarian Res, 2017-04-04;10(1):25.
Species: Human
Sample Types: Serum
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Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivity
Authors: I Bergström, AK Lundberg, S Jönsson, E Särndahl, J Ernerudh, L Jonasson
PLoS ONE, 2017-03-22;12(3):e0174177.
Species: Human
Sample Types: Plasma
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Aberrant expression of interleukin-10 in rheumatoid arthritis: Relationship with IL10 haplotypes and autoantibodies
Authors: J Hernández-, E Oregón-Rom, M Vázquez-Vi, S García-Are, Y Valle, JR Padilla-Gu, IV Román-Fern, CA Palafox-Sá, GE Martínez-B, JF Muñoz-Vall
Cytokine, 2017-02-27;95(0):88-96.
Species: Human
Sample Types: Serum
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Ghrelin and hormonal markers under exercise training in patients with heart failure with preserved ejection fraction: results from the Ex-DHF pilot study
Authors: TD Trippel, V Holzendorf, M Halle, G Gelbrich, K Nolte, A Duvinage, S Schwarz, T Rutscher, J Wiora, R Wachter, C Herrmann-L, HD Duengen, G Hasenfu beta, B Pieske, F Edelmann
ESC Heart Fail, 2016-08-31;4(1):56-65.
Species: Human
Sample Types: Serum
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Genome-Wide Association Study Identifies That the ABO Blood Group System Influences Interleukin-10 Levels and the Risk of Clinical Events in Patients with Acute Coronary Syndrome.
Authors: Johansson A, Alfredsson J, Eriksson N, Wallentin L, Siegbahn A
PLoS ONE, 2015-11-24;10(11):e0142518.
Species: Human
Sample Types: Plasma
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Acute phase IL-10 plasma concentration associates with the high risk sources of cardiogenic stroke.
Authors: Arponen O, Muuronen A, Taina M, Sipola P, Hedman M, Jakala P, Vanninen R, Pulkki K, Mustonen P
PLoS ONE, 2015-04-29;10(4):e0120910.
Species: Human
Sample Types: Plasma
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Mucosal effects of tenofovir 1% gel.
Authors: Hladik F, Burgener A, Ballweber L, Gottardo R, Vojtech L, Fourati S, Dai J, Cameron M, Strobl J, Hughes S, Hoesley C, Andrew P, Johnson S, Piper J, Friend D, Ball T, Cranston R, Mayer K, McElrath M, McGowan I
Elife, 2015-02-03;4(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Psychological stress exerts effects on pathogenesis of hepatitis B via type-1/type-2 cytokines shift toward type-2 cytokine response.
Authors: He Y, Gao H, Li X, Zhao Y
PLoS ONE, 2014-08-21;9(8):e105530.
Species: Human
Sample Types: Plasma
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The impact of two different transfusion strategies on patient immune response during major abdominal surgery: a preliminary report.
Authors: Theodoraki K, Markatou M, Rizos D, Fassoulaki A
J Immunol Res, 2014-04-03;2014(0):945829.
Species: Human
Sample Types: Plasma
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Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
Obesity (Silver Spring), 2011-09-08;19(11):2130-6.
Species: Human
Sample Types: Plasma
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Whole-Body UVB Irradiation during Allogeneic Hematopoietic Cell Transplantation Is Safe and Decreases Acute Graft-versus-Host Disease.
Authors: Kreutz M, Karrer S, Hoffmann P
J. Invest. Dermatol., 2011-08-18;132(1):179-87.
Species: Human
Sample Types: Serum
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Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
PLoS ONE, 2011-07-28;6(7):e22748.
Species: Human
Sample Types: Plasma
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SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
Diabetes, 2010-01-27;59(4):829-35.
Species: Human
Sample Types: Plasma
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Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
Atherosclerosis, 2009-12-02;210(2):536-41.
Species: Human
Sample Types: Plasma
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Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
Transplantation, 2009-11-15;88(9):1109-16.
Species: Human
Sample Types: Urine
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Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
Authors: Sun Y, Yi D, Wang Y, Zheng R, Sun G, Wang J, Liu Y, Ren J, Wang Y, Zhang S, Gu C, Pei J
Cytokine, 2009-07-24;47(3):206-13.
Species: Human
Sample Types: Plasma
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Interleukin-18 is a strong predictor of cardiovascular events in elderly men with the metabolic syndrome: synergistic effect of inflammation and hyperglycemia.
Authors: Troseid M, Seljeflot I, Hjerkinn EM, Arnesen H
Diabetes Care, 2008-12-17;32(3):486-92.
Species: Human
Sample Types: Serum
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Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
Authors: Poulsen TD, Andersen LW, Steinbruchel D, Gotze JP, Jorgensen OS, Olsen NV
J. Cardiothorac. Vasc. Anesth., 2008-10-22;23(3):316-23.
Species: Human
Sample Types: Plasma
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In vivo transmigrated monocytes from patients with stable coronary artery disease have a reduced expression of CD11b.
Authors: Paulsson JM, Dadfar E, Held C, Jacobson SH, Lundahl J
Clin. Exp. Immunol., 2008-05-05;153(2):196-204.
Species: Human
Sample Types: Serum
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Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
Ann. Surg., 2008-02-01;247(2):320-6.
Species: Human
Sample Types: Plasma
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Duffy antigen modifies the chemokine response in human endotoxemia.
Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
Crit. Care Med., 2008-01-01;36(1):159-65.
Species: Human
Sample Types: Plasma
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Co-occurrence of IgA antibodies against ethanol metabolites and tissue transglutaminase in alcohol consumers: correlation with proinflammatory cytokines and markers of fibrogenesis.
Authors: Koivisto H, Hietala J, Anttila P, Niemela O
Dig. Dis. Sci., 2007-06-28;53(2):500-5.
Species: Human
Sample Types: Serum
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Anti-inflammatory cytokines in gingival crevicular fluid in patients with periodontitis and rheumatoid arthritis: a preliminary report.
Authors: Bozkurt FY, Yetkin Ay Z, Berker E, Tepe E, Akkus S
Cytokine, 2006-09-18;35(3):180-5.
Species: Human
Sample Types:
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The activity of N-acetyl-beta-D-glucosaminidase and tumor necrosis factor-alpha at early stage of diabetic retinopathy development in type 1 diabetes mellitus children.
Authors: Mysliwiec M, Zorena K, Balcerska A, Mysliwska J, Lipowski P, Raczynska K
Clin. Biochem., 2006-04-05;39(8):851-6.
Species: Human
Sample Types: Serum
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Dysregulated monocyte iron homeostasis and erythropoietin formation in patients with anemia of chronic disease.
Authors: Theurl I, Mattle V, Seifert M, Mariani M, Marth C, Weiss G
Blood, 2006-01-24;107(10):4142-8.
Species: Human
Sample Types: Serum
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Recombinant human antithrombin inhibits thrombin formation and interleukin 6 release in human endotoxemia.
Authors: Leitner JM, Firbas C, Mayr FB, Reiter RA, Steinlechner B, Jilma B
Clin. Pharmacol. Ther., 2006-01-01;79(1):23-34.
Species: Human
Sample Types: Plasma
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Interleukin-8, monocyte chemoattractant protein-1 and IL-10 in the vitreous fluid of patients with proliferative diabetic retinopathy.
Authors: Hernandez C, Segura RM, Fonollosa A, Carrasco E, Francisco G, Simo R
Diabet. Med., 2005-06-01;22(6):719-22.
Species: Human
Sample Types: Vitreous Humor
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Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
Microbes Infect., 2005-02-24;7(3):427-36.
Species: Human
Sample Types: Serum
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Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
Transpl. Int., 2005-02-01;18(2):177-85.
Species: Human
Sample Types: Plasma
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Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
Authors: Jensen J, Krakauer M, Sellebjerg F
Cytokine, 2005-01-07;29(1):24-30.
Species: Human
Sample Types: Plasma
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Comparative effects of AT1-antagonism and angiotensin-converting enzyme inhibition on markers of inflammation and platelet aggregation in patients with coronary artery disease.
Authors: Schieffer B, Bunte C, Witte J, Hoeper K, Boger RH, Schwedhelm E, Drexler H
J. Am. Coll. Cardiol., 2004-07-21;44(2):362-8.
Species: Human
Sample Types: Serum
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Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors.
Authors: Alter P, Maisch B
Z Kardiol, 2004-07-01;93(7):524-32.
Species: Human
Sample Types: Serum
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Immunodetection of a hepatitis C virus (HCV) antigen and Thl/Th2 cytokines in cerebrospinal fluid of meningitis patients.
Authors: Attallah AM, Ibrahim GG
J Immunoassay Immunochem, 2004-01-01;25(4):313-20.
Species: Human
Sample Types: Serum
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Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis.
Authors: Benson M, Strannegard IL, Wennergren G, Strannegard O
Pediatr Allergy Immunol, 2000-02-01;11(1):20-8.
Species: Human
Sample Types: Nasal Lavage Fluid
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