Human IL-10 R beta Alexa Fluor® 488-conjugated Antibody Summary
Met20-Ser220
Accession # Q08334
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑10 R beta in Human PBMC Monocytes by Flow Cytometry. Human PBMC monocytes were stained with Mouse Anti-Human IL-10 R beta Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB874G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-10 R beta
IL-10 mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL-10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta is expressed ubiquitously. IL-10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.
- Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
- Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
- Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
- Liu, Y. et al. (1994) J. Immunol. 152:1821.
- Lutfalla, G. et al. (1993) Genomics 16:366.
- Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for Human IL-10 R beta Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Inhibition of Type III Interferon Expression in Intestinal Epithelial Cells-A Strategy Used by Coxsackie B Virus to Evade the Host's Innate Immune Response at the Primary Site of Infection?
Authors: VM Stone, EE Ringqvist, PG Larsson, E Domsgen, U Holmlund, E Sverremark, M Flodström-
Microorganisms, 2021-01-05;9(1):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Symmetric Arginine Dimethylation Is Selectively Required for mRNA Splicing and the Initiation of Type I and Type III Interferon Signaling
Authors: PJ Metz, KA Ching, T Xie, P Delgado Cu, S Niessen, JH Tatlock, K Jensen-Per, BW Murray
Cell Rep, 2020-02-11;30(6):1935-1950.e8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Hepatitis B Virus e Antigen Activates the Suppressor of Cytokine Signaling 2 to Repress Interferon Action
Authors: Y Yu, P Wan, Y Cao, W Zhang, J Chen, L Tan, Y Wang, Z Sun, Q Zhang, Y Wan, Y Zhu, F Liu, K Wu, Y Liu, J Wu
Sci Rep, 2017-05-11;7(1):1729.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Influence of Canonical and Non-Canonical IFNLR1 Isoform Expression on Interferon Lambda Signaling
Authors: Evans JG, Novotny LA, Meissner EG
Viruses
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This antibody was used to stain human peripheral blood for analysis by flow cytometry. Monocytes were identified from CD45+ leukocytes and IL-10Rbeta expression was measured in the FITC channel. This antibody achieves consistent fluorescence which is maintained after fixation
