Human IL-10 R beta Antibody

Catalog #: MAB8741 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8741
MAB8741-SP

All IL-10 R beta Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-10 R beta Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-10 R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, ~10% cross‑reactivity with recombinant human (rh) IL-10 R and rhIFN-alpha / beta R1 is observed and no cross-reactivity with rhIFN-gamma RI and rhIFN-gamma R2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 90227

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta
Met20-Ser220
Accession # Q08334

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑10 R beta Fc Chimera (Catalog # 874-RB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R beta

IL-10 R beta, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) residue type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a short 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4^th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta is expressed ubiquitously. IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

References

Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
Liu, Y. et al. (1994) J. Immunol. 152:1821.
Lutfalla, G. et al. (1993) Genomics 16:366.
Kotenko, S.V. et al. (1997) EMBO J. 16:5894.

Long Name

Interleukin 10 Receptor beta

Entrez Gene IDs

3588 (Human); 16155 (Mouse)

Alternate Names

CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 R beta; IL-10 receptor subunit beta; IL10R beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10Rb; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4