Human IL-10 R beta APC-conjugated Antibody Summary
Met20-Ser220
Accession # Q08334
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑10 R beta in Human PBMC Monocytes by Flow Cytometry. Human PBMC monocytes were stained with Mouse Anti-Human IL-10 R beta APC-conjugated Monoclonal Antibody (Catalog # FAB874A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-10 R beta
IL-10 mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL-10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta is expressed ubiquitously. IL-10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.
- Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
- Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
- Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
- Liu, Y. et al. (1994) J. Immunol. 152:1821.
- Lutfalla, G. et al. (1993) Genomics 16:366.
- Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Product Datasheets
Citation for Human IL-10 R beta APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Diminished secretion and function of IL-29 is associated with impaired IFN-? response of neonatal plasmacytoid dendritic cells
Authors: L Wisgrill, I Wessely, A Netzl, L Pummer, K Sadeghi, A Spittler, A Berger, E Förster-Wa
J. Leukoc. Biol., 2019-06-18;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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