Human IL-11 Antibody

Catalog #: AB-218-NA
2 Citations Datasheet / COA / SDS

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AB-218-NA

Cell Proliferation Induced by IL‑11 and Neutralization by Human IL‑11 Antibody.

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All IL-11 Antibodies

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Citations (2)

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Human IL-11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant mouse IL-11 is observed.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑11
Pro22-Leu199
Accession # P20809

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑11 (Catalog # 218-IL)

Neutralization

Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND₅₀) is typically 10-20 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑11.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑11 and Neutralization by Human IL‑11 Antibody. Recombinant Human IL-11 (Catalog # 218-IL) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-11 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-11 Polyclonal Antibody (Catalog # AB-218-NA). The ND₅₀ is typically 10-20 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-11

Interleukin 11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The human IL-11 cDNA encodes a 199 amino acid residue precursor polypeptide with a 21 amino acid residue hydrophobic signal that is processed proteolytically to generate the 178 amino acid residue mature protein. IL-11 contains no cysteine residues or potential glycosylation sites.

IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G₀ period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell. IL-11, in the presence of IL-3 or SCF, has also been shown to stimulate erythropoiesis. Among nonhematopoietic cell populations, IL-11, like IL-6 and LIF, can stimulate the synthesis of hepatic acute-phase proteins. Consistent with the in vitro functions of IL‑11, in vivo administration of rhIL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors.

IL-11 exerts its biological activities through binding to a specific high-affinity receptor having an apparent molecular mass of 150 kDa. Although the IL-11 binding subunit of the receptor complex has not yet been cloned, evidence suggests that, similar to IL-6, leukemia inhibitory factor, oncostatin M, and ciliary neurotrophic factor, IL-11 utilizes the IL-6 signal transducer, gp130, for signal transduction.

Long Name

Interleukin 11

Entrez Gene IDs

3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)

Alternate Names

Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin

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Product Datasheet

COA

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Citations for Human IL-11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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