Home / IL-12/IL-23 p40 / Human IL-12/IL-23 p40 Antibody

Human IL-12/IL-23 p40 Antibody

Catalog #: MAB1510
13 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1510-SP
MAB1510-500
MAB1510-100
IL‑17 Secretion Induced by IL‑23 and Neutralization by Human IL‑12/IL‑23 p40 Antibody.
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Citations (13)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-12/IL-23 p40 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-12 (p40/p35 heterodimer), IL-23 (p40/p19 heterodimer), and p40 homodimers in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-12 p40 or rpIL-12.
Source
Monoclonal Mouse IgG1 Clone # 24901
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12 p40/p35 heterodimer
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑23-induced IL‑17 secretion in mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 0.75 ng/mL Recombinant Human IL‑23 and 10 ng/mL Recombinant Mouse IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL‑17 Secretion Induced by IL‑23 and Neutralization by Human IL‑12/IL‑23 p40 Antibody. View Larger

IL‑17 Secretion Induced by IL‑23 and Neutralization by Human IL‑12/IL‑23 p40 Antibody. In the presence of Recombinant Mouse IL-2 (10 ng/mL, Catalog # 402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB1510). The ND50 is typically 0.02-0.1 µg/mL. This antibody also neutralizes Human IL-12 induced activity in human PBLs. The ND50 is approximately 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Human IL-12.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T-cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDA (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Long Name
Interleukin 12/Interleukin 23 p40
Entrez Gene IDs
3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)
Alternate Names
CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

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Citations for Human IL-12/IL-23 p40 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration
    Authors: Guo Y, Chomiak AA, Hong Y et al.
    iScience
  2. Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death
    Authors: Gasset-Rosa F, Lu S, Yu H et al.
    Neuron
  3. Nde1 is required for heterochromatin compaction and stability in neocortical neurons
    Authors: Chomiak AA, Guo Y, Kopsidas CA et al.
    iScience
  4. Sarcoma IL-12 overexpression facilitates NK cell immunomodulation
    Authors: MJ Rademacher, A Cruz, M Faber, RAA Oldham, D Wang, JA Medin, NJ Schloemer
    Scientific Reports, 2021-04-15;11(1):8321.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Capture, Neutralization
  5. Characterization of the interaction between human decidua parietalis mesenchymal stem/stromal cells and natural killer cells
    Authors: MH Abumaree, E Bahattab, A Alsadoun, A Al Dosaima, FM Abomaray, T Khatlani, B Kalionis, MF El-Muzaini, AO Alawad, AS AlAskar
    Stem Cell Res Ther, 2018-04-12;9(1):102.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Phage-assisted continuous evolution of proteases with altered substrate specificity
    Authors: MS Packer, HA Rees, DR Liu
    Nat Commun, 2017-10-16;8(1):956.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
    Authors: Sommer A, Fabri M
    PLoS ONE, 2015-06-24;10(6):e0130395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014-03-31;2014(0):593562.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  9. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
    Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
    J Immunol, 2012-11-14;189(12):5590-601.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Engineering of stable bispecific antibodies targeting IL-17A and IL-23
    Authors: Robert Mabry, Katherine E. Lewis, Margaret Moore, Patricia A. McKernan, Thomas R. Bukowski, Kristen Bontadelli et al.
    Protein Engineering, Design and Selection
  11. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
    Authors: Rao DA, Tracey KJ, Pober JS
    J. Immunol., 2007-11-15;179(10):6536-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
    Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
    J. Immunol., 2005-02-15;174(4):2376-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Cholesterol Metabolism Is a Druggable Axis that Independently Regulates Tau and Amyloid-b in iPSC-Derived Alzheimer's Disease Neurons
    Authors: van der Kant R, Langness VF, Herrera CM et al.
    Cell Stem Cell

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Reviews for Human IL-12/IL-23 p40 Antibody

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Human IL-12/IL-23 p40 Antibody
By Anonymous on 11/23/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human
Immunocytochemistry/Immunofluorescence Human IL-12/IL-23 p40 Antibody MAB1510
Human IL-12/IL-23 p40 Antibody
By Anonymous on 11/04/2017
Application: ELISA Sample Tested: S2 Drosophila embryonic epithelial cell line Species: Drosophila