Home / IL-12 p70 / Human IL-12 p70 DuoSet ELISA

Human IL-12 p70 DuoSet ELISA

Catalog #: DY1270
54 Citations 10 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY1270
DY1270-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
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Human IL-12 p70 ELISA Standard Curve
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Product Details
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Citations (54)
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Supplemental Products
Reviews (10)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human IL-12 p70 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-12 p70. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (Catalog # DY005)

 

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Normal Goat Serum: (Catalog # DY005)

 

Scientific Data

N/A Human IL-12 p70 ELISA Standard Curve View Larger

Human IL-12 p70 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Long Name:
Interleukin 12 p70
Entrez Gene IDs:
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names:
CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent with NGS, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human IL-12 p70 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

54 Citations: Showing 1 - 10
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  1. Impaired Proliferation of CD8+ T Cells Stimulated with Monocyte-Derived Dendritic Cells Previously Matured with Thapsigargin-Stimulated LAD2 Human Mast Cells
    Authors: Kalkusova, K;Taborska, P;Stakheev, D;Rataj, M;Smite, S;Darras, E;Albo, J;Bartunkova, J;Vannucci, L;Smrz, D;
    Journal of immunology research
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Neutrophil extracellular traps promote immunopathogenesis of virus-induced COPD exacerbations
    Authors: Katsoulis, O;Toussaint, M;Jackson, MM;Mallia, P;Footitt, J;Mincham, KT;Meyer, GFM;Kebadze, T;Gilmour, A;Long, M;Aswani, AD;Snelgrove, RJ;Johnston, SL;Chalmers, JD;Singanayagam, A;
    Nature communications
    Species: Human
    Sample Types: Sputum
  3. TNF? induced by DNA-sensing in macrophage compromises retinal pigment epithelial (RPE) barrier function
    Authors: Twarog, M;Schustak, J;Xu, Y;Coble, M;Dolan, K;Esterberg, R;Huang, Q;Saint-Geniez, M;Bao, Y;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
  4. Increased Pro Th1 And Th17 Transcriptional Activity In Patients With Severe COVID-19
    Authors: M Jovanovic, S Sekulic, M Jocic, M Jurisevic, N Gajovic, M Jovanovic, N Arsenijevi, M Jovanovic, M Mijailovic, M Milosavlje, I Jovanovic
    International Journal of Medical Sciences, 2023-02-21;20(4):530-541.
    Species: Human
    Sample Types: Serum
  5. Rare Phytocannabinoids Exert Anti-Inflammatory Effects on Human Keratinocytes via the Endocannabinoid System and MAPK Signaling Pathway
    Authors: D Tortolani, C Di Meo, S Standoli, F Ciaramella, S Kadhim, E Hsu, C Rapino, M Maccarrone
    International Journal of Molecular Sciences, 2023-02-01;24(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Signal strength of STING activation determines cytokine plasticity and cell death in human monocytes
    Authors: D Kabelitz, M Zarobkiewi, M Heib, R Serrano, M Kunz, G Chitadze, D Adam, C Peters
    Scientific Reports, 2022-10-24;12(1):17827.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. HydrAd: A Helper-Dependent Adenovirus Targeting Multiple Immune Pathways for Cancer Immunotherapy
    Authors: A Rosewell S, C Porter, G Biegert, L Jatta, M Suzuki
    Oncogene, 2022-06-02;14(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. IFN-gamma and TNF Induce Senescence and a Distinct Senescence-Associated Secretory Phenotype in Melanoma
    Authors: L Homann, M Rentschler, E Brenner, K Böhm, M Röcken, T Wieder
    Cells, 2022-04-30;11(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Ascorbic acid supports ex vivo generation of plasmacytoid dendritic cells from circulating hematopoietic stem cells
    Authors: A Laustsen, RM van der Sl, A Gris-Olive, SS Hernández, E Cemalovic, HQ Tang, LH Pedersen, N Uldbjerg, MR Jakobsen, RO Bak
    Elife, 2021-09-02;10(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. New Biomarker in Chagas Disease: Extracellular Vesicles Isolated from Peripheral Blood in Chronic Chagas Disease Patients Modulate the Human Immune Response
    Authors: RP Madeira, LM Dal'Mas Ro, P de Cássia, C Mady, BM Ianni, AC Torrecilha
    Journal of Immunology Research, 2021-01-11;2021(0):6650670.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India
    Authors: SK Biswas, M Mittal, E Sinha, V Singh, N Arela, B Bajaj, PK Tiwari, VM Katoch, KK Mohanty
    Journal of Immunology Research, 2020-12-16;2020(0):1019639.
    Species: Human
    Sample Types: Serum
  12. Monocytes differentiated into macrophages and dendritic cells in the presence of human IFN-&lambda3 or IFN-&lambda4 show distinct phenotypes
    Authors: M De, A Bhushan, S Chinnaswam
    J Leukoc Biol, 2020-11-17;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation
    Authors: ME Morrissey, R Byrne, C Nulty, NH McCabe, N Lynam-Lenn, CT Butler, S Kennedy, D O'Toole, J Larkin, P McCormick, B Mehigan, MC Cathcart, J Lysaght, JV Reynolds, EJ Ryan, MR Dunne, J O'Sullivan
    BMC Cancer, 2020-06-17;20(1):566.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Evaluation of a Recombinant Newcastle Disease Virus Expressing Human IL12 against Human Breast Cancer
    Authors: Z Mohamed Am, MA Che Ani, SW Tan, SK Yeap, NB Alitheen, SUF Syed Najmu, J Kalyanasun, SC Chan, A Veerakumar, SL Chia, K Yusoff
    Sci Rep, 2019-09-30;9(1):13999.
    Species: Chicken, Human
    Sample Types: Cell Culture Supernates
  15. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy
    Authors: M Pascual-Ga, E Bonfill-Te, E Planas-Rig, C Rubio-Pere, R Iurlaro, A Arias, I Cuartas, A Sala-Hojma, L Escudero, F Martínez-R, I Huber-Ruan, P Nuciforo, L Pedrosa, C Marques, I Braña, E Garralda, M Vieito, M Squatrito, E Pineda, F Graus, C Espejo, J Sahuquillo, J Tabernero, J Seoane
    Nat Commun, 2019-06-11;10(1):2416.
    Species: Human
    Sample Types: Tissue Homogenates
  16. In Vitro Induction of T Helper 17 Cells by Synergistic Activation of Human Monocyte-Derived Langerhans Cell-Like Cells with Bacterial Agonists
    Authors: R Gramlich, E Aliahmadi, M Peiser
    Int J Mol Sci, 2019-03-19;20(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. D-2-Hydroxyglutarate and L-2-Hydroxyglutarate Inhibit IL-12 Secretion by Human Monocyte-Derived Dendritic Cells
    Authors: I Ugele, ZE Cárdenas-C, K Hammon, M Wehrstein, C Bruss, K Peter, K Singer, E Gottfried, J Boesch, P Oefner, K Dettmer, K Renner, M Kreutz
    Int J Mol Sci, 2019-02-10;20(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Cervical cancer-instructed stromal fibroblasts enhance IL-23 expression in dendritic cells to support expansion of Th17 cells
    Authors: B Walch-Rück, R Ströder, L Theobald, J Pahne-Zepp, S Hegde, YJ Kim, RM Bohle, I Juhasz-Bös, EF Solomayer, S Smola
    Cancer Res., 2019-01-29;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Plasmodium falciparum Treated with Artemisinin-based Combined Therapy Exhibits Enhanced Mutation, Heightened Cortisol and TNF-? Induction
    Authors: AO Idowu, S Bhattachar, S Gradus, W Oyibo, Z George, C Black, J Igietseme, AA Azenabor
    Int J Med Sci, 2018-09-07;15(13):1449-1457.
    Species: Human
    Sample Types: Plasma
  20. Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function
    Authors: A Laustsen, RO Bak, C Krapp, L Kjær, JH Egedahl, CC Petersen, S Pillai, HQ Tang, N Uldbjerg, M Porteus, NR Roan, M Nyegaard, PW Denton, MR Jakobsen
    Nat Commun, 2018-08-30;9(1):3525.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. A novel susceptibility locus in the IL12B region is associated with the pathophysiology of Takayasu arteritis through IL-12p40 and IL-12p70 production
    Authors: T Nakajima, H Yoshifuji, M Shimizu, K Kitagori, K Murakami, R Nakashima, Y Imura, M Tanaka, K Ohmura, F Matsuda, C Terao, T Mimori
    Arthritis Res. Ther., 2017-09-06;19(1):197.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Minimal hepatic encephalopathy is associated with expansion and activation of CD(4+)CD28(-), Th22 and Tfh and B lymphocytes
    Authors: A Mangas-Los, R García-Gar, A Urios, D Escudero-G, J Tosca, R Giner-Durá, MA Serra, C Montoliu, V Felipo
    Sci Rep, 2017-07-27;7(1):6683.
    Species: Human
    Sample Types: Serum
  23. Dexamethasone induced inhibition of Dectin-1 activation of antigen presenting cells is mediated via STAT-3 and NF-?B signaling pathways.
    Authors: Philipp Kotthoff, Annkristin Heine, Stefanie Andrea Erika Held, Peter Brossart
    Scientific Reports, 2017-07-03;0(0):2045-2322.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. The effects of exogenous lipid on THP-1 cells: an in vitro model of airway aspiration?
    Authors: YA Hayman, LR Sadofsky, JD Williamson, SP Hart, AH Morice
    ERJ Open Res, 2017-03-20;3(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Th1/Th17-Related Cytokines and Chemokines and Their Implications in the Pathogenesis of Pemphigus Vulgaris
    Authors: RP Timoteo, MV da Silva, CB Miguel, DA Silva, JD Catarino, V Rodrigues, H Sales-Camp, CJ Freire Oli
    Mediators Inflamm., 2017-02-22;2017(0):7151285.
    Species: Human
    Sample Types: Serum
  26. Acanthamoeba castellanii (Genotype T4) Stimulates the Production of Interleukin-10 as well as Pro-inflammatory Cytokines in THP-1 Cells, Human Peripheral Blood Mononuclear Cells and Human Monocyte-Derived Macrophages
    Infect Immun, 2016-09-19;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype
    Sci Rep, 2016-07-19;6(0):29712.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Pleural Effusions from Patients with Mesothelioma Induce Recruitment of Monocytes and Their Differentiation into M2 Macrophages.
    Authors: Chene A, d'Almeida S, Blondy T, Tabiasco J, Deshayes S, Fonteneau J, Cellerin L, Delneste Y, Gregoire M, Blanquart C
    J Thorac Oncol, 2016-07-12;11(10):1765-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015-08-14;195(6):2818-28.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
    Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
    PLoS ONE, 2015-03-20;10(3):e0120948.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2.
    Authors: Jaguin M, Fardel O, Lecureur V
    PLoS ONE, 2015-02-24;10(2):e0116560.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. The effects of cytokines on spontaneous hepatitis B surface antigen seroconversion in chronic hepatitis B virus infection.
    Authors: Wu J, Hsu H, Chiu Y, Chen H, Ni Y, Chang M
    J Immunol, 2014-12-12;194(2):690-6.
    Species: Human
    Sample Types: Serum
  33. Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
    Authors: Tudor C, Bruns H, Daniel C, Distel L, Hartmann A, Gerbitz A, Buettner M
    PLoS ONE, 2014-12-03;9(12):e114345.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Tumour microenvironment of both early- and late-stage colorectal cancer is equally immunosuppressive.
    Authors: O'Toole A, Michielsen A, Nolan B, Tosetto M, Sheahan K, Mulcahy H, Winter D, Hyland J, O'Connell P, Fennelly D, O'Donoghue D, O'Sullivan J, Doherty G, Ryan E
    Br J Cancer, 2014-07-24;111(5):927-32.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Effect of CIK on multidrug-resistance reversal and increasing the sensitivity of ADR in K562/ADR cells.
    Authors: Wang L, Deng Q, Wang J, Bai X, Xiao X, Lv H, Zhao M, Liu P
    Oncol Lett, 2014-07-10;8(4):1778-1782.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
    Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
    PLoS ONE, 2014-06-16;9(6):e99612.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Anti-inflammatory effects of green soybean extract irradiated with visible light.
    Authors: Tanaka K, Ohgo Y, Katayanagi Y, Yasui K, Hiramoto S, Ikemoto H, Nakata Y, Miyoshi N, Isemura M, Ohashi N, Imai S
    Sci Rep, 2014-04-22;4(0):4732.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells.
    Authors: Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H
    Br J Haematol, 2014-01-15;165(1):134-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.
    Authors: Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D
    PLoS ONE, 2013-12-30;8(12):e82867.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Two wheat decapeptides prevent gliadin-dependent maturation of human dendritic cells.
    Authors: Giordani L, Del Pinto T, Vincentini O, Felli C, Silano M, Viora M
    Exp Cell Res, 2013-11-19;321(2):248-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
    Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
    J. Bone Miner. Res., 2011-08-01;26(8):1891-903.
    Species: Human
    Sample Types: Serum
  42. Human uterine epithelial cell secretions regulate dendritic cell differentiation and responses to TLR ligands.
    Authors: Ochiel DO, Ghosh M, Fahey JV
    J. Leukoc. Biol., 2010-04-12;88(3):435-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
    Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
    J. Immunol., 2009-12-15;183(12):8176-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009-03-02;119(10):1424-32.
    Species: Human
    Sample Types: Plasma
  45. The 3'UTR 1188 A/C polymorphism in the interleukin-12p40 gene (IL-12B) is associated with lepromatous leprosy in the west of Mexico.
    Authors: Alvarado-Navarro A, Montoya-Buelna M, Munoz-Valle JF, Lopez-Roa RI, Guillen-Vargas C, Fafutis-Morris M
    Immunol. Lett., 2008-04-28;118(2):148-51.
    Species: Human
    Sample Types: Serum
  46. Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells.
    Authors: Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M
    FASEB J., 2008-03-25;22(8):2747-57.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007-12-01;117(12):3664-3672.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. The differential effect of Eriobotrya japonica hydrophilic leaf extract on cytokines production and modulation.
    Authors: Matalka KZ, Ali D, Khawad AE, Qa'dan F
    Cytokine, 2007-11-26;40(3):235-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007-09-11;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. The direct effects of Toll-like receptor ligands on human NK cell cytokine production and cytotoxicity.
    Authors: Lauzon NM, Mian F, MacKenzie R, Ashkar AA
    Cell. Immunol., 2006-10-17;241(2):102-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression.
    Authors: Macagno A, Molteni M, Rinaldi A
    J. Exp. Med., 2006-05-22;203(6):1481-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists.
    Authors: Fritz JH, Girardin SE, Fitting C, Werts C, Mengin-Lecreulx D, Caroff M, Cavaillon JM, Philpott DJ, Adib-Conquy M
    Eur. J. Immunol., 2005-08-01;35(8):2459-70.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model.
    Authors: Weigt H, Muhlradt PF, Larbig M, Krug N, Braun A
    J. Immunol., 2004-05-15;172(10):6080-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody.
    Authors: Asselin-Paturel C, Brizard G, Pin JJ, Briere F, Trinchieri G
    J. Immunol., 2003-12-15;171(12):6466-77.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Average Rating: 3.9 (Based on 10 Reviews)

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Human IL-12 p70 DuoSet ELISA
By Hongbo Gao on 07/18/2024
Sample Tested: EDTA Plasma

How hard can it be for the company to include goat serum in the kit rather than sell it separately? It’s not a very common reagent, and other human cytokine kits don’t use it at all.

Human IL-12 p70 DuoSet ELISA
By Anonymous on 11/15/2022
Sample Tested: Serum

The standard curve was good, but the human serum samples were all undetectable.

Human IL-12 p70 DuoSet ELISA DY1270
Human IL-12 p70 DuoSet ELISA
By Anonymous on 05/12/2021
Sample Tested: Cell culture supernatant
Human IL-12 p70 DuoSet ELISA DY1270
Human IL-12 p70 DuoSet ELISA
By Anonymous on 02/22/2018
Sample Tested: THP-1 human acute monocytic leukemia cell line
Human IL-12 p70 DuoSet ELISA DY1270
Human IL-12 p70 DuoSet ELISA
By Balaji Mahender on 01/18/2018
Sample Tested: EDTA Plasma
Human IL-12 p70 DuoSet ELISA
By BOCHATON Thomas on 11/21/2017
Sample Tested: Serum

We used human serum at a dilution of 1:1. The test was good and worked well, but IL-12p70 was detectable in only 15% of our patients, in our conditions.

Human IL-12 p70 DuoSet ELISA DY1270
Human IL-12 p70 DuoSet ELISA
By Anonymous on 11/02/2017
Sample Tested: Cell culture supernatant
Human IL-12 p70 DuoSet ELISA
By Anonymous on 06/02/2016
Sample Tested: Human cells

High Quality Elisa we use for our release testing

Human IL-12 p70 DuoSet ELISA
By Anonymous on 04/22/2016
Sample Tested: Serum and Plasma

Human serum and plasma samples are not possible to run with this kit. All samples are lower than the lowest standard.

Human IL-12 p70 DuoSet ELISA, 15 Plate
By Anonymous on 10/26/2015
Sample Tested: Dendritic cells Species: Human

Standard curve is shown.
Buffer: kit dilution buffer
Dilution: 1/100

Human IL-12 p70 DuoSet ELISA, 15 Plate DY1270