Best Seller

Human IL-12 p70 DuoSet ELISA

R&D Systems | Catalog # DY1270

R&D Systems
100% Guarantee

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

31.2-2000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human IL-12 p70 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all IL-12 p70 ELISA Kits »

Product Summary for Human IL-12 p70 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-12 p70. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human IL-12 p70 DuoSet ELISA

Human IL-12 p70 ELISA Standard Curve

Human IL-12 p70 ELISA Standard Curve

Kit Contents for Human IL-12 p70 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (Catalog # DY005)

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Normal Goat Serum: (Catalog # DY005)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

IL-12 (Interleukin-12) is a secreted heterodimeric cytokine that contains disulfide-linked p35 and p40 subunits. The p40 subunit is also a subunit of the heterodimeric IL-23. IL-12 is produced by macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, microglia, and non-germinal center B cells. It acts  through a receptor complex that contains the ligand binding IL-12 R alpha 1 and the signal transducing IL-12 R alpha 2. IL-12 can enhance cytotoxic activity and induce Interferon-gamma production in NK cells, T cells and dendritic epidermal T cells. In conjunction with IL-23 and IL-27, IL-12 promotes the development of a Th1 immune response.

Long Name

Interleukin 12 p70

Alternate Names

IL12 p70

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Gene Symbol

IL12A

Additional IL-12 p70 Products

Product Documents for Human IL-12 p70 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL-12 p70 DuoSet ELISA

For research use only

Citations for Human IL-12 p70 DuoSet ELISA

Customer Reviews for Human IL-12 p70 DuoSet ELISA (10)

3.9 out of 5
10 Customer Ratings
5 Stars
50%
4 Stars
20%
3 Stars
10%
2 Stars
10%
1 Stars
10%

Have you used Human IL-12 p70 DuoSet ELISA?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 of 10 reviews Showing All
Filter By:
Clear Filters X
  • Human IL-12 p70 DuoSet ELISA
    Name: Hongbo Gao
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 07/18/2024
    How hard can it be for the company to include goat serum in the kit rather than sell it separately? It’s not a very common reagent, and other human cytokine kits don’t use it at all.
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Human IL-12 p70 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 11/15/2022
    The standard curve was good, but the human serum samples were all undetectable.
    Human IL-12 p70 DuoSet ELISA DY1270
  • Human IL-12 p70 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 05/12/2021
    Human IL-12 p70 DuoSet ELISA DY1270
  • Human IL-12 p70 DuoSet ELISA
    Name: Anonymous
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Verified Customer | Posted 02/22/2018
    Human IL-12 p70 DuoSet ELISA DY1270
  • Human IL-12 p70 DuoSet ELISA
    Name: Balaji Mahender
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 01/18/2018
  • Human IL-12 p70 DuoSet ELISA
    Name: BOCHATON Thomas
    Sample Tested: Serum
    Verified Customer | Posted 11/21/2017
    We used human serum at a dilution of 1:1. The test was good and worked well, but IL-12p70 was detectable in only 15% of our patients, in our conditions.
    Human IL-12 p70 DuoSet ELISA DY1270
  • Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 11/02/2017
  • Human IL-12 p70 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Human cells
    Verified Customer | Posted 06/02/2016
    High Quality Elisa we use for our release testing
  • Human IL-12 p70 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 04/22/2016
    Human serum and plasma samples are not possible to run with this kit. All samples are lower than the lowest standard.
  • Human IL-12 p70 DuoSet ELISA, 15 Plate
    Name: Anonymous
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Standard curve is shown. <br />Buffer: kit dilution buffer<br />Dilution: 1/100
    Human IL-12 p70 DuoSet ELISA DY1270

There are no reviews that match your criteria.

Showing  1 - 5 of 10 reviews Showing All

Protocols

View specific protocols for Human IL-12 p70 DuoSet ELISA (DY1270):

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent with NGS, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits