Human IL-12 R beta 2 Antibody

Detection of Human IL‑12 R beta 2 by Western Blot. Western blot shows lysates of human CD4^{+in vitrodifferentiated Th1 cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human IL-12 R beta 2 Monoclonal Antibody (Catalog # MAB19591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-12 R beta 2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.}

Detection of Human IL-12 R beta 2 by Western Blot Reduced IL‐35 in patients with ITP. A) The level of plasma IL‐35 by ELISA (n = 12 in the control group, n = 26 in the ITP group). B) The concentration of IL‐35 in the bone marrow of ITP patients and healthy controls by ELISA (n = 22 in the control group, n = 10 in the ITP group). C) Expression of IL‐35 receptor subunit IL‐12R beta 2 in CD41/CD61+ cells by flow cytometry (n = 10). D) The mean fluorescence intensity of IL‐12R beta 2 on day 10 by flow cytometry (n = 10). E) Expression of IL‐35 receptor subunit gp130 in CD41/CD61+ cells by flow cytometry (n = 10). F) The mean fluorescence intensity of gp130 on day 10 by flow cytometry (n = 10). G) The protein levels of IL‐35 receptor subunits IL‐12R beta 2 and gp130 in CD41/CD61+ cells (n = 6). H) CD41 (green), IL‐12R beta 2/ gp130 (red), and DAPI (blue) staining of CD41/CD61+ cells (600×; bar: 20 µm). Data information: Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significant difference, using Student‘s t‐test (A, B, D, F) or ANOVA (C, E). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38225757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human IL-12 R beta 2 by Western Blot IL‐35 stimulates megakaryopoiesis in vitro. A) Expression of IL‐35 receptor subunit IL‐12R beta 2 in CD41/CD61+ cells from ITP patients by flow cytometry (n = 10). B) The mean fluorescence intensity of IL‐12R beta 2 on day 10 by flow cytometry (n = 10). C) Expression of IL‐35 receptor subunit gp130 in CD41/CD61+ cells from ITP patients by flow cytometry (n = 10). D) The mean fluorescence intensity of gp130 on day 10 by flow cytometry (n = 10). E) The protein levels of IL‐35 receptor subunits IL‐12R beta 2 and gp130 in CD41/CD61+ cells (n = 6). F) CD41 (green), IL‐12R beta 2/ gp130 (red), and DAPI (blue) staining of CD41/CD61+ cells (600×; bar: 20 µm). G) (Left) Representative images of megakaryocyte colonies (100×; bar: 100 µm). (Right) The number of megakaryocyte colonies (n = 3). Data information: Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significant difference, using ANOVA (A, C, E, F) or Student‘s t‐test (B, D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38225757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human IL-12 R beta 2 by Immunocytochemistry/ Immunofluorescence IL‐35 stimulates megakaryopoiesis in vitro. A) Expression of IL‐35 receptor subunit IL‐12R beta 2 in CD41/CD61+ cells from ITP patients by flow cytometry (n = 10). B) The mean fluorescence intensity of IL‐12R beta 2 on day 10 by flow cytometry (n = 10). C) Expression of IL‐35 receptor subunit gp130 in CD41/CD61+ cells from ITP patients by flow cytometry (n = 10). D) The mean fluorescence intensity of gp130 on day 10 by flow cytometry (n = 10). E) The protein levels of IL‐35 receptor subunits IL‐12R beta 2 and gp130 in CD41/CD61+ cells (n = 6). F) CD41 (green), IL‐12R beta 2/ gp130 (red), and DAPI (blue) staining of CD41/CD61+ cells (600×; bar: 20 µm). G) (Left) Representative images of megakaryocyte colonies (100×; bar: 100 µm). (Right) The number of megakaryocyte colonies (n = 3). Data information: Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significant difference, using ANOVA (A, C, E, F) or Student‘s t‐test (B, D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38225757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human IL-12 R beta 2 by Immunocytochemistry/ Immunofluorescence Reduced IL‐35 in patients with ITP. A) The level of plasma IL‐35 by ELISA (n = 12 in the control group, n = 26 in the ITP group). B) The concentration of IL‐35 in the bone marrow of ITP patients and healthy controls by ELISA (n = 22 in the control group, n = 10 in the ITP group). C) Expression of IL‐35 receptor subunit IL‐12R beta 2 in CD41/CD61+ cells by flow cytometry (n = 10). D) The mean fluorescence intensity of IL‐12R beta 2 on day 10 by flow cytometry (n = 10). E) Expression of IL‐35 receptor subunit gp130 in CD41/CD61+ cells by flow cytometry (n = 10). F) The mean fluorescence intensity of gp130 on day 10 by flow cytometry (n = 10). G) The protein levels of IL‐35 receptor subunits IL‐12R beta 2 and gp130 in CD41/CD61+ cells (n = 6). H) CD41 (green), IL‐12R beta 2/ gp130 (red), and DAPI (blue) staining of CD41/CD61+ cells (600×; bar: 20 µm). Data information: Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significant difference, using Student‘s t‐test (A, B, D, F) or ANOVA (C, E). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38225757), licensed under a CC-BY license. Not internally tested by R&D Systems.