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Human IL-12 R beta 2 Antibody

Catalog #: AF1959
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1959
AF1959-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-12 R beta 2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse IL-12 R beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human IL‑12 R beta 1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2
Cys28-Asn622
Accession # Q99665
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL

Recombinant Human IL‑12 R beta 2 Fc Chimera (Catalog # 1959-B2)

Recombinant Mouse IL-12 R beta 2 Fc Chimera

Blockade of Receptor-ligand Interaction
In a functional ELISA, 1.5-5 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human IL-12 (Catalog # 219-IL) to immobilized Recombinant Human IL-12 R beta 2 Fc Chimera (Catalog # 1959-B2) coated at 5 µg/mL (100 µL/well). At 83.3 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 2

Interleukin 12 (IL-12), the founding member of the IL-12 family of heterodimeric cytokines, is composed of two disulfide-linked 35 kDa and 40 kDa subunits. The 35 kDa subunit (p35) is a alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also a subunit of IL-23. IL-12 is an essential mediator of cellular-immunity that induces T cells and natural-killer cells to produce IFN-gamma. It is also required for the expansion and activation Th1 cells (1, 2).

The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 Receptor beta 1 (IL-12 R beta 1) and IL-12 Receptor beta 2 (IL‑12 R beta 2) subunits. IL-12 R beta 1 is a 100 kDa protein that is also a subunit of the IL-23 receptor complex. It binds IL-12/IL-23 p40 and is associated with Tyk2. IL-12 R beta 2 is a 130 kDa protein that interacts with p35 and is associated with Jak2. Both receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 2 cDNA encodes a 862 amino acid (aa) residue protein with a putative 27 aa residue signal peptide that is cleaved to generate the mature protein with a 595 aa residue extracellular domain, a 24 aa residue transmembrane domain and a 216 aa residue cytoplasmic region. Human and mouse IL-12 R beta 2 share 68% amino acid sequence identity. Whereas IL-12 R beta 1 expression has been detected in activated T cells, NK cells and B cells, the expression of IL-12 R beta 2 is more restricted. Among T cells, IL-12 R beta 2 is absent on naive T cells. Activation of T cells via TCR up‑regulates IL‑12 R beta 2 expression on human Th1 but not Th2 cells (1‑4).

References
  1. Trinchieri, G. et al. (2003) Immunity 19:641. 
  2. Brombacher, F. et al. (2003) Trends in Immunol. 23:207. 
  3. Trinchieri, G. (2003) Nature Reviews Immunol. 3:133.
  4. Rogge, L. et al. (1997) J. Exp. Med. 185:825.
Long Name
Interleukin 12 Receptor beta 2
Entrez Gene IDs
3595 (Human); 16162 (Mouse)
Alternate Names
IL-12 R beta 2; IL-12 receptor beta 2; IL-12 receptor subunit beta-2; IL12R beta 2; IL-12R subunit beta-2; IL12RB2; IL-12Rb2; IL-12R-beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain; interleukin-12 receptor subunit beta-2

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Citations for Human IL-12 R beta 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Differential Induction of IFN-? and Modulation of CD112 and CD54 Expression Govern the Magnitude of NK Cell IFN-? Response to Influenza A Viruses
    Authors: LM Kronstad, C Seiler, R Vergara, SP Holmes, CA Blish
    J. Immunol., 2018-08-24;0(0):.
  2. Predictive biomarkers for the responsiveness of recurrent glioblastomas to activated killer cell immunotherapy
    Authors: S Hwang, J Lim, H Kang, JY Jeong, JG Joung, J Heo, D Jung, K Cho, HJ An
    Cell & bioscience, 2023-01-24;13(1):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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