Human IL-13 R alpha 1 Biotinylated Antibody

Catalog #: BAF152
2 Citations Datasheet / COA / SDS

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BAF152

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Detection of IL‑13 R alpha 1 in Human Granulocytes by Flow Cytometry.

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Human IL-13 R alpha 1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-13 R alpha 1 in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL‑13 R alpha 2, recombinant mouse (rm) IL-13 R alpha, rhIL-4 R, rhIL-5 R alpha, and rhIL-9 R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1
Ala27-Thr343 (Thr130Ile)
Accession # Q5JSL4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑13 R alpha 1 Fc Chimera (Catalog # 146-IR)

Flow Cytometry

2.5 µg/10⁶ cells

See below

Human IL-13 R alpha 1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human IL‑13 R alpha 1 Antibody (Catalog # MAB146)

Standard: Recombinant Human IL-13 R alpha 1 Fc Chimera Protein, CF (Catalog # 146-IR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑13 R alpha 1 in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Human IL-13 Ra1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF152, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13 R alpha 1

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL-13 R alpha 1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region, and a 59 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL-13 R alpha 1 is also closely related to that of IL‑13 R alpha 2. IL‑13 R alpha 1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.

References

Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.

Long Name

Interleukin 13 Receptor alpha 1

Entrez Gene IDs

3597 (Human); 16164 (Mouse)

Alternate Names

bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1 antigen; CD213a1; CT19; IL-13 R alpha 1; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; IL13R alpha 1; IL-13R subunit alpha-1; IL13R; IL13RA; IL-13Ra; IL13RA1; IL-13Ra1; IL-13R-alpha-1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; NR4

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Citations for Human IL-13 R alpha 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Engineered IL13 variants direct specificity of IL13Ralpha2-targeted CAR T cell therapy
Authors: LA Stern, S Gholamin, I Moraga, X Yang, S Saravanaku, JR Cohen, R Starr, B Aguilar, V Salvary, JC Hibbard, A Kalbasi, JK Shepphird, J O'Hearn, KC Garcia, CE Brown
Proceedings of the National Academy of Sciences of the United States of America, 2022-08-08;119(33):e2112006119.
Species: Yeast
Sample Types: Whole Cell
Applications: Flow Cytometry
Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
J. Immunol., 2004-12-01;173(11):6592-602.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC