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Human IL-15 ELISA Kit - Quantikine

Catalog #: D1500
58 Citations 3 Reviews Datasheet / COA / SDS
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S1500
PD1500
D1500
Control Products Available
QC26: Quantikine Immunoassay Control Set 1247 for Human IL-15
Human IL-15 ELISA Cell Culture Supernate Standard Curve
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Citations (58)
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Reviews (3)
Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-15 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.25 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
2 pg/mL
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-15
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Thirty-seven serum and plasma samples from apparently healthy volunteers were evaluated for the presence of human IL-15 in this assay. All samples measured less than the lowest IL-15 standard, 3.91 pg/mL. No medical histories were available for the donors used in this study. 

Cell Culture Supernates:
Human peripheral blood mononuclear cells (5 x 106 cells/mL) were cultured in RPMI supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of natural human IL-15. All samples measured less than the lowest IL-15 standard, 3.91 pg/mL. 

HuT 102 human cutaneous T cell lymphocyte cells were cultured in a 1:1 mixture of DMEM with high glucose and Hamm's F12 supplemented with a 150-fold final dilution of Nutridoma NS (Boehringer Mannheim) and a 500-fold dilution of defined lipid concentrate (Gibco). An aliquot of the cell culture supernate was removed, assayed for human IL-15, and measured 153 pg/mL.

Product Summary

The Quantikine Human IL-15 Immunoassay is a 4.25 or 4.5 hour solid phase ELISA designed to measure human IL-15 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-15 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-15 expressed by HuT-102 cells showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-15.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 7.2 22.9 131 9.2 29 154
Standard Deviation 0.3 1.3 5.8 0.7 1.6 10.2
CV% 4.2 5.7 4.4 7.6 5.5 6.6

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 7.7 24.7 140 9.9 32.8 184
Standard Deviation 0.3 1.3 4.8 0.9 2.7 12.2
CV% 3.9 5.3 3.4 9.1 8.2 6.6

Recovery

The recovery of human IL-15 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 93-104
EDTA Plasma (n=5) 99 90-112
Heparin Plasma (n=5) 100 91-107
Serum (n=5) 98 92-109

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of IL-15 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Human IL-15 ELISA Linearity

Scientific Data

N/A Human IL-15 ELISA Cell Culture Supernate Standard Curve View Larger

Human IL-15 ELISA Cell Culture Supernate Standard Curve

N/A Human IL-15 ELISA Serum/Plasma Standard Curve View Larger

Human IL-15 ELISA Serum/Plasma Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-15

IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.

Long Name:
Interleukin 15
Entrez Gene IDs:
3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names:
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well.
  7.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 45 minutes.
  8.   Aspirate and wash 4 times.
    9. 200 µL Substrate Solution
  9.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-15 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

58 Citations: Showing 1 - 10
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  1. Assessment of Metabolic, Inflammatory, and Immunological Disorders Using a New Panel of Plasma Parameters in People Living with HIV Undergoing Antiretroviral Therapy-A Retrospective Study
    Authors: Szyma?ska, B;Knysz, B;Ciep?ucha, H;Piwowar, A;
    Journal of clinical medicine
    Species: Human
    Sample Types: Plasma
  2. An alternative fully human anti-BCMA CAR-T shows response for relapsed or refractory multiple myeloma with anti-BCMA CAR-T exposures previously
    Authors: Wang, Q;Wei, R;Guo, S;Min, C;Zhong, X;Huang, H;Cheng, Z;
    Cancer gene therapy
    Species: Human
    Sample Types: Whole Blood
  3. Unlocking the potential of allogeneic V?2 T cells for ovarian cancer therapy through CD16 biomarker selection and CAR/IL-15 engineering
    Authors: Lee, D;Dunn, ZS;Guo, W;Rosenthal, CJ;Penn, NE;Yu, Y;Zhou, K;Li, Z;Ma, F;Li, M;Song, TC;Cen, X;Li, YR;Zhou, JJ;Pellegrini, M;Wang, P;Yang, L;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Efficacy of gilteritinib in comparison with alectinib for the treatment of ALK-rearranged non-small cell lung cancer
    Authors: Ando, C;Ichihara, E;Nishi, T;Morita, A;Hara, N;Takada, K;Nakasuka, T;Watanabe, H;Kano, H;Nishii, K;Makimoto, G;Kondo, T;Ninomiya, K;Fujii, M;Kubo, T;Ohashi, K;Matsuoka, KI;Hotta, K;Tabata, M;Maeda, Y;Kiura, K;
    Cancer science
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Unique Profile of Proinflammatory Cytokines in Plasma of Drug-Naïve Individuals with Advanced HIV/TB Co-Infection
    Authors: Nosik, M;Belikova, MG;Ryzhov, K;Avdoshina, D;Sobkin, A;Zverev, V;Svitich, O;
    Viruses
    Species: Human
    Sample Types: Plasma
  6. Assessment of Interleukin-15 (IL-15) Concentration in Children with Idiopathic Nephrotic Syndrome
    Authors: A Bade?ski, M Bade?ska, E ?wi?tochow, A Janek, A Gliwi?ska, A Morawiec-K, M Szczepa?sk
    International Journal of Molecular Sciences, 2023-04-10;24(8):.
    Species: Human
    Sample Types: Serum, Urine
  7. Human Hematopoietic Stem Cell Engrafted IL-15 Transgenic NSG Mice Support Robust NK Cell Responses and Sustained HIV-1 Infection
    Authors: SA Abeynaike, TR Huynh, A Mehmood, T Kim, K Frank, K Gao, C Zalfa, A Gandarilla, L Shultz, S Paust
    Viruses, 2023-01-27;15(2):.
    Species: Humanized Mouse
    Sample Types: Serum
  8. Co-expression IL-15 receptor alpha with IL-15 reduces toxicity via limiting IL-15 systemic exposure during CAR-T immunotherapy
    Authors: Y Zhang, Q Zhuang, F Wang, C Zhang, C Xu, A Gu, WH Zhong, Y Hu, X Zhong
    Oncogene, 2022-09-27;20(1):432.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Delivery of CAR-T cells in a transient injectable stimulatory hydrogel niche improves treatment of solid tumors
    Authors: AK Grosskopf, L Labanieh, DD Klysz, GA Roth, P Xu, O Adebowale, EC Gale, CK Jons, JH Klich, J Yan, CL Maikawa, S Correa, BS Ou, AI d'Aquino, JR Cochran, O Chaudhuri, CL Mackall, EA Appel
    Science Advances, 2022-04-08;8(14):eabn8264.
    Species: Mouse
    Sample Types: Serum
  10. Effector memory CD8 T cell response elicits Hepatitis E Virus genotype 3 pathogenesis in the elderly
    Authors: H El Costa, J Gouilly, F Abravanel, E Bahraoui, JM Peron, N Kamar, N Jabrane-Fe, J Izopet
    PloS Pathogens, 2021-02-22;17(2):e1009367.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Relationship between CD4 T cell turnover, cellular differentiation and HIV persistence during ART
    Authors: C Bacchus-So, M Fitch, J Symons, M Abdel-Mohs, DB Reeves, R Hoh, M Stone, J Hiatt, P Kim, A Chopra, H Ahn, VA York, DL Cameron, FM Hecht, JN Martin, SA Yukl, S Mallal, PU Cameron, SG Deeks, JT Schiffer, SR Lewin, MK Hellerstei, JM McCune, PW Hunt
    PloS Pathogens, 2021-01-19;17(1):e1009214.
    Species: Human
    Sample Types: Plasma
  12. The Diverse Potential of Gluten from Different Durum Wheat Varieties in Triggering Celiac Disease: A Multilevel In Vitro, Ex Vivo and In Vivo Approach
    Authors: F Gaiani, S Graziano, F Boukid, B Prandi, L Bottarelli, A Barilli, A Dossena, N Marmiroli, M Gullì, GL de'Angelis, S Sforza
    Nutrients, 2020-11-20;12(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Exhaled Biomarkers in Idiopathic Pulmonary Fibrosis-A Six-Month Follow-Up Study in Patients Treated with Pirfenidone
    Authors: K Jaskiewicz, K Mycroft, M Maskey-War, K Paralusz, N Siemiez, P Nejman-Gry, M Barnas, R Krenke, K Gorska
    J Clin Med, 2020-08-05;9(8):.
    Species: Human
    Sample Types: EBC, Exhaled Breath Condensate (EBC)
  14. Reliability and suitability of physiological exercise response and recovery markers
    Authors: T Reichel, TK Bo beta lau, J Palmowski, K Eder, R Ringseis, FC Mooren, R Walscheid, E Bothur, S Samel, T Frech, M Philippe, K Krüger
    Sci Rep, 2020-07-17;10(1):11924.
    Species: Human
    Sample Types: Plasma
  15. Effects of Regular Taekwondo Intervention on Oxidative Stress Biomarkers and Myokines in Overweight and Obese Adolescents
    Authors: HT Roh, SY Cho, WY So
    Int J Environ Res Public Health, 2020-04-06;17(7):.
    Species: Human
    Sample Types: Plasma
  16. Plasma Myokine Concentrations After Acute Exercise in Non-obese and Obese Sedentary Women
    Authors: L Garneau, SA Parsons, SR Smith, EE Mulvihill, LM Sparks, C Aguer
    Front Physiol, 2020-02-18;11(0):18.
    Species: Human
    Sample Types: Plasma
  17. Diabetes alters immune response patterns to acute melioidosis in humans
    Authors: B Kronsteine, P Chaichana, M Sumonwiriy, K Jenjaroen, FR Chowdhury, S Chumseng, P Teparrukku, D Limmathuro, NPJ Day, P Klenerman, SJ Dunachie
    Eur. J. Immunol., 2019-05-08;0(0):.
    Species: Human
    Sample Types: Serum
  18. Myokine Response to High-Intensity Interval vs. Resistance Exercise: An Individual Approach
    Authors: Z He, Y Tian, PL Valenzuela, C Huang, J Zhao, P Hong, Z He, S Yin, A Lucia
    Front Physiol, 2018-12-03;9(0):1735.
    Species: Human
    Sample Types: Serum
  19. A 2-Week Specific Volleyball Training Supported by the Whole Body Cryostimulation Protocol Induced an Increase of Growth Factors and Counteracted Deterioration of Physical Performance
    Authors: J Jaworska, K Micielska, M Koz?owska, K Wnorowski, J Skrobecki, L Radzimi?sk, A Babi?ska, E Rodziewicz, G Lombardi, E Ziemann
    Front Physiol, 2018-11-28;9(0):1711.
    Species: Human
    Sample Types: Serum
  20. Intact regulation of muscle expression and circulating levels of myokines in response to exercise in patients with�type 2 diabetes
    Authors: R Sabaratnam, AJT Pedersen, JM Kristensen, A Handberg, JFP Wojtaszews, K Højlund
    Physiol Rep, 2018-06-01;6(12):e13723.
    Species: Human
    Sample Types: Plasma
  21. Altered levels of memory T cell subsets and common ?c cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment
    Authors: A Rajamanick, S Munisankar, Y Bhootra, CK Dolla, K Thiruvenga, TB Nutman, S Babu
    PLoS Negl Trop Dis, 2018-05-24;12(5):e0006481.
    Species: Human
    Sample Types: Plasma
  22. Interleukin 15 concentrations in follicular fluid and their effect on oocyte maturation in subfertile women undergoing intracytoplasmic sperm injection
    Authors: S Spanou, D Kalogianni, E Zapanti, M Gazouli, IA Sfontouris, C Siristatid, G Mastorakos
    J. Assist. Reprod. Genet., 2018-04-04;0(0):.
    Species: Human
    Sample Types: Follicular Fluid
  23. Scalable, cGMP-compatible purification of extracellular vesicles carrying bioactive human heterodimeric IL-15/lactadherin complexes
    Authors: DC Watson, BC Yung, C Bergamasch, B Chowdhury, J Bear, D Stellas, A Morales-Ka, JC Jones, BK Felber, X Chen, GN Pavlakis
    J Extracell Vesicles, 2018-02-28;7(1):1442088.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Activating and inhibitory receptors on natural killer cells in patients with systemic lupus erythematosis-regulation with interleukin-15
    Authors: SJ Lin, ML Kuo, HS Hsiao, PT Lee, JY Chen, JL Huang
    PLoS ONE, 2017-10-12;12(10):e0186223.
    Species: Human
    Sample Types: Serum
  25. Skeletal muscle IL-15/IL-15R? and myofibrillar protein synthesis after resistance exercise
    Authors: A Pérez-Lópe, J McKendry, M Martin-Rin, D Morales-Al, B Pérez-Köhl, D Valadés, J Buján, JAL Calbet, L Breen
    Scand J Med Sci Sports, 2017-05-26;0(0):.
    Species: Human
    Sample Types: Serum
  26. Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment
    Authors: NP Kumar, VV Banurekha, D Nair, S Babu
    PLoS ONE, 2017-04-27;12(4):e0176495.
    Species: Human
    Sample Types: Plasma
  27. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
    J Immunol, 2016-08-10;197(5):1621-30.
    Species: Human
    Sample Types: Serum
  28. Immune Recovery after Allogeneic Hematopoietic Stem Cell Transplantation Following Flu-TBI versus TLI-ATG Conditioning.
    Authors: Hannon M, Beguin Y, Ehx G, Servais S, Seidel L, Graux C, Maertens J, Kerre T, Daulne C, De Bock M, Fillet M, Ory A, Willems E, Gothot A, Humblet-Baron S, Baron F
    Clin Cancer Res, 2015-03-16;21(14):3131-9.
    Species: Human
    Sample Types: Plasma
  29. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
    Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
    Gene Ther, 2014-10-02;22(1):76-86.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. The systemic cytokine environment is permanently altered in multiple myeloma.
    Authors: Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J
    PLoS ONE, 2013-03-27;8(3):e58504.
    Species: Human
    Sample Types: Plasma
  31. Decreased IL-7 responsiveness is related to oxidative stress in HIV disease.
    Authors: Kalinowska M, Bazdar D, Lederman M, Funderburg N, Sieg S
    PLoS ONE, 2013-03-07;8(3):e58764.
    Species: Human
    Sample Types: Serum
  32. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012-07-09;7(7):e40620.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Adoptive transfer of autologous natural killer cells leads to high levels of circulating natural killer cells but does not mediate tumor regression.
    Authors: Parkhurst MR, Riley JP, Dudley ME, Rosenberg SA
    Clin. Cancer Res., 2011-08-15;17(19):6287-97.
    Species: Human
    Sample Types: Serum
  34. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011-05-19;6(5):e19988.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha.
    Authors: Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y
    Blood, 2011-02-08;117(15):4032-40.
    Species: Human
    Sample Types: Serum
  36. Plasma levels of IL-7 and IL-15 after reduced intensity conditioned allo-SCT and relationship to acute GVHD.
    Authors: Thiant S, Labalette M, Trauet J, Coiteux V, de Berranger E, Dessaint JP, Yakoub-Agha I
    Bone Marrow Transplant., 2010-12-06;46(10):1374-81.
    Species: Human
    Sample Types: Plasma
  37. Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma.
    Authors: Vyth-Dreese FA, Sein J, van de Kasteele W, Dellemijn TA, van den Bogaard C, Nooijen WJ, de Gast GC, Haanen JB, Bex A
    Clin. Exp. Immunol., 2010-10-05;162(3):447-59.
    Species: Human
    Sample Types: Plasma
  38. Plasma levels of IL-7 and IL-15 in the first month after myeloablative BMT are predictive biomarkers of both acute GVHD and relapse.
    Authors: Thiant S, Yakoub-Agha I, Magro L, Trauet J, Coiteux V, Jouet JP, Dessaint JP, Labalette M
    Bone Marrow Transplant., 2010-03-01;45(10):1546-52.
    Species: Human
    Sample Types: Plasma
  39. Phenotype markers and cytokine intracellular production by CD8+ gammadelta T lymphocytes do not support a regulatory T profile in Behcet's disease patients and healthy controls.
    Authors: Clemente A, Cambra A, Munoz-Saa I, Crespi C, Pallares L, Juan A, Matamoros N, Julia MR
    Immunol. Lett., 2010-02-16;129(2):57-63.
    Species: Human
    Sample Types: Serum
  40. Single-dose nevirapine exposure affects T cell response and cytokine levels in HIV type 1-infected women.
    Authors: Shalekoff S, Meddows-Taylor S, Schramm DB, Gray G, Sherman G, Coovadia A, Kuhn L, Tiemessen CT
    AIDS Res. Hum. Retroviruses, 2009-10-01;25(10):1049-53.
    Species: Human
    Sample Types: Plasma
  41. Safety and immunologic effects of IL-15 administration in nonhuman primates.
    Authors: Berger C, Berger M, Hackman RC, Gough M, Elliott C, Jensen MC, Riddell SR
    Blood, 2009-07-15;114(12):2417-26.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Types: Plasma
  42. Enhancing the in vivo expansion of adoptively transferred EBV-specific CTL with lymphodepleting CD45 monoclonal antibodies in NPC patients.
    Authors: Louis CU, Straathof K, Bollard CM, Gerken C, Huls MH, Gresik MV, Wu MF, Weiss HL, Gee AP, Brenner MK, Rooney CM, Heslop HE, Gottschalk S
    Blood, 2008-10-29;113(11):2442-50.
    Species: Human
    Sample Types: Plasma
  43. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008-08-01;181(3):2227-37.
    Species: Human
    Sample Types: Serum
  44. Isolation and characterization of CD8+ regulatory T cells in multiple sclerosis.
    Authors: Correale J, Villa A
    J. Neuroimmunol., 2008-01-30;195(1):121-34.
    Species: Human
    Sample Types: Serum
  45. Circulating interleukin-15 and RANTES chemokine in Parkinson's disease.
    Authors: Rentzos M, Nikolaou C, Andreadou E, Paraskevas GP, Rombos A, Zoga M, Tsoutsou A, Boufidou F, Kapaki E, Vassilopoulos D
    Acta Neurol. Scand., 2007-12-01;116(6):374-9.
    Species: Human
    Sample Types: Serum
  46. Serum interleukin-15 level is a useful predictor of the complications and mortality in severe acute pancreatitis.
    Authors: Ueda T, Takeyama Y, Yasuda T, Shinzeki M, Sawa H, Nakajima T, Takase K, Matsumoto I, Fujita T, Ajiki T, Fujino Y, Kuroda Y
    Surgery, 2007-09-01;142(3):319-26.
    Species: Human
    Sample Types: Serum
  47. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis.
    Authors: Lepej SZ, Misic-Majerus L, Jeren T, Rode OD, Remenar A, Sporec V, Vince A
    Acta Neurol. Scand., 2007-02-01;115(2):109-14.
    Species: Human
    Sample Types: CSF
  48. Levels of IL-15 in serum and cerebrospinal fluid of patients with Behçet's disease.
    Authors: Hamzaoui K, Hamzaoui A, Ghorbel I, Khanfir M, Houman H
    Scand. J. Immunol., 2006-12-01;64(6):655-60.
    Species: Human
    Sample Types: Serum
  49. Juxtacrine function of interleukin-15/interleukin-15 receptor system in tumour derived human B-cell lines.
    Authors: Tsukamoto K, Huang YC, Dorsey WC, Carns B, Sharma V
    Clin. Exp. Immunol., 2006-12-01;146(3):559-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
  51. Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
    Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
    Leuk. Res., 2004-10-01;28(10):1023-31.
    Species: Human
    Sample Types: Serum
  52. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
    Authors: Ripley D, Shoup B, Majewski A
    Gynecol. Oncol., 2004-03-01;92(3):761-8.
    Species: Human
    Sample Types: Tissue Homogenates
  53. NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
    Authors: Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
    J. Immunol., 2004-01-01;172(1):130-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
    Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
    J. Immunol., 2002-05-01;168(9):4326-32.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
    Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
    Blood, 2002-01-01;99(1):224-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis.
    Authors: Ong PY, Hamid QA, Travers JB, Strickland I, Al Kerithy M, Boguniewicz M, Leung DY
    J. Immunol., 2002-01-01;168(1):505-10.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000-07-17;192(2):219-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
    Authors: Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T
    J. Infect. Dis., 1999-03-01;179(3):646-52.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Types: Plasma

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Human IL-15 Quantikine ELISA Kit
By Anonymous on 02/25/2023
Sample Tested: Serum-free Cell Culture Supernates
Human IL-15 Quantikine ELISA Kit
By Anonymous on 01/22/2020
Sample Tested: Serum and Plasma
Human IL-15 Quantikine ELISA Kit
By Anonymous on 01/10/2020
Sample Tested: Serum and Plasma