Human IL-17/IL-17A PerCP-conjugated Antibody

Catalog #: IC3171C
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC3171C-100
IC3171C-025

Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry.

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All IL-17/IL-17A Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-17/IL-17A PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, recombinant canine Il-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.

Source

Monoclonal Mouse IgG₁ Clone # 41802

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL-17/IL-17A antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-17/IL-17A PerCP-conjugated Monoclonal Antibody (Catalog # IC3171C) and Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on control antibody staining (Catalog # IC002C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17/IL-17A

Interleukin-17 (IL-17) is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human IL-17/IL-17A PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Carboxylesterase 1 mediates a distinctive metabolic profile of dendritic cells to attain an inflammatory phenotype
Authors: Elfiky, AMI;López Canñizares, J;Li, J;Li Yim, AYF;Verhoeven, AJ;Ghiboub, M;de Jonge, WJ;
Journal of leukocyte biology
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature.
Authors: Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J
PLoS ONE, 2013-12-05;8(12):e81983.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry