Human IL-17F PE-conjugated Antibody

Name: Human IL-17F PE-conjugated Antibody
Brand: R&D Systems
SKU: IC13351P

Catalog #: IC13351P Datasheet / COA / SDS

Discontinued Product

IC13351P has been discontinued.

View all IL-17F products.

Detection of IL‑17F in Human Blood Lymphocytes by Flow Cytometry.

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All IL-17F Antibodies

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Human IL-17F PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-17F in direct ELISAs. Does not cross-react with recombinant human IL‑17A, B, C, D, or E.

Source

Monoclonal Mouse IgG_2B Clone # 197301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL-17F
Arg31-Gln163
Accession # Q96PD4

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL‑17F antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

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Detection of IL‑17F in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-17F PE-conjugated Monoclonal Antibody (Catalog # IC13351P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17F

Interleukin-17F (IL-17F) is a disulfide-linked dimer belonging to the IL-17 family. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions.

Long Name

Interleukin 17F

Entrez Gene IDs

112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)

Alternate Names

Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F