Human IL-17RA/IL-17R Antibody

Catalog #: MAB177
20 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB177-SP
MAB177-100
MAB177-500

Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.

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Citations (20)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-17RA/IL-17R Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed

Source

Monoclonal Mouse IgG₁ Clone # 133617

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R
Leu33-Trp320
Accession # Q96F46

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑17 RA/IL‑17 R Fc Chimera (Catalog # 177-IR)

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Knockout Validated

IL-17 RA/IL-17 R is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in IL-17 RA/IL-17 R knockout K562 cell line.

Neutralization

Measured by its ability to neutralize IL‑17/IL‑17A-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.4-2 μg/mL in the presence of 40 ng/mL Recombinant Human IL‑17/IL‑17A.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). View our protocol for Staining Membrane-associated Proteins.

Neutralization

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CXCL1/GRO alpha Secretion Induced by IL‑17/IL‑17A and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL-17/IL-17A induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GROa DuoSet (DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-17/IL-17A (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177). The ND₅₀ is typically 0.4-2 µg/mL.

Knockout Validated

IL-17 RA/IL-17 R Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line.

View Larger

IL-17 RA/IL-17 R Specificity is Shown by Flow Cytometry in Knockout Cell Line. IL-17 RA/IL-17 R knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). No staining in the IL-17 RA/IL-17 R knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

References

Yao, Z. et al. (1997) Cytokine 9:794.
Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Long Name

Interleukin 17 Receptor

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Alternate Names

CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-17RA/IL-17R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Alpha-synuclein fine-tunes neuronal response to pro-inflammatory cytokines
Authors: Sigutova, V;Xiang, W;Regensburger, M;Winner, B;Prots, I;
Brain, behavior, and immunity
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry
Evidence for anti-inflammatory effects and modulation of neurotransmitter metabolism by Salvia officinalis L
Authors: G Margetts, S Kleidonas, NS Zaibi, MS Zaibi, KD Edwards
BMC complementary medicine and therapies, 2022-05-12;22(1):131.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Impact of Adalimumab Treatment on Interleukin-17 and Interleukin-17 Receptor Expression in Skin and Synovium of Psoriatic Arthritis Patients with Mild Psoriasis
Authors: JW Bolt, AW van Kuijk, MBM Teunissen, D van der Co, S Aarrass, DM Gerlag, PP Tak, MG van de San, MC Lebre, LGM van Baarse
Biomedicines, 2022-01-29;10(2):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Interleukin-17A Triggers the Release of Platelet-Derived Factors Driving Vascular Endothelial Cells toward a Pro-Angiogenic State
Authors: A Gatsiou, K Sopova, A Tselepis, K Stellos
Cells, 2021-07-22;10(8):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Cutting Edge: IL-17B Uses IL-17RA and IL-17RB to Induce Type 2 Inflammation from Human Lymphocytes
Authors: Vladimir Ramirez-Carrozzi, Naruhisa Ota, Arivazhagan Sambandam, Kit Wong, Jason Hackney, Nadia Martinez-Martin et al.
The Journal of Immunology
Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology
Authors: PA Szabo, A Goswami, DM Mazzuca, K Kim, DB O'Gorman, DA Hess, ID Welch, HA Young, B Singh, JK McCormick, SM Haeryfar
J. Immunol, 2017-02-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Autophagy impacts on oxaliplatin-induced hepatocarcinoma apoptosis via the IL-17/IL-17R-JAK2/STAT3 signaling pathway
Authors: J Wu, J Guo, Q Cao, Y Wang, J Chen, Z Wang, Z Yuan
Oncol Lett, 2016-12-08;13(2):770-776.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
Eur J Immunol, 2015-12-14;46(2):440-5.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination.
Authors: Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z
Eur J Immunol, 2015-02-01;45(2):442-51.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Cells
Applications: Neutralization
Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
Mucosal Immunol, 2011-12-21;5(2):140-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
Authors: Ramirez-Carrozzi V, Sambandam A, Luis E
Nat. Immunol., 2011-10-12;12(12):1159-66.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

TH17 cytokines induce human airway smooth muscle cell migration.
Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma.
Authors: Corrigan CJ, Wang W, Meng Q, Fang C, Wu H, Reay V, Lv Z, Fan Y, An Y, Wang YH, Liu YJ, Lee TH, Ying S
Proc. Natl. Acad. Sci. U.S.A., 2011-01-04;108(4):1579-84.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
Int. J. Cancer, 2009-09-01;125(5):1092-101.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

IL-17 induces monocyte migration in rheumatoid arthritis.
Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
J. Immunol., 2009-03-15;182(6):3884-91.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
Circulation, 2009-03-02;119(10):1424-32.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
Authors: Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P
J. Immunol., 2008-01-01;180(1):655-63.
Species: Human
Sample Types: RNA, Whole Tissue
Applications: IHC-P, Western Blot

CD molecules 2006--human cell differentiation molecules.
Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
J. Immunol. Methods, 2006-12-04;319(1):1-5.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005-11-01;175(9):6177-89.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Toll-like receptor 4-mediated lymphocyte influx induces neonatal necrotizing enterocolitis.
Authors: Egan CE, Sodhi CP, Good M et al.
J. Clin. Invest.