Human IL-17RA/IL-17R PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB177P-100
FAB177P-025

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Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.
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Product Details
Citations (8)
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Human IL-17RA/IL-17R PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
Source
Monoclonal Mouse IgG1 Clone # 133617
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-17 R
Leu33-Trp320
Accession # Q96F46
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-17 R antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R PE-conjugated Monoclonal Antibody (Catalog # FAB177P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Western Blot Detection of IL-17RA/IL-17R by Western Blot View Larger

Detection of IL-17RA/IL-17R by Western Blot Evaluation of IL-17 signaling in TWIST1-knockdown BC cells compared to control cells. (A) Protein levels of Act1 and IL-17RA in ShCTRL and ShTWIST cells. GAPDH was used to ensure that the same sample amounts were loaded for immunoblotting. Densitometric analysis was done using ImageJ software. (B) Levels of secreted IL-6 and IL-8 proteins in the supernatant of ShCTRL and ShTWIST cells. Data are shown as the mean (±SD). * p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34830027), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17RA/IL-17R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

References
  1. Yao, Z. et al. (1997) Cytokine 9:794.
  2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
Long Name
Interleukin 17 Receptor
Entrez Gene IDs
23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)
Alternate Names
CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A

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Citations for Human IL-17RA/IL-17R PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
    Authors: BRB Pires, R Binato, GM Ferreira, S Corrêa, B Du Rocher, D Bulzico, S Crocamo, EC Dos Santos, LG Lima, E Abdelhay
    International Journal of Molecular Sciences, 2021-11-10;22(22):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Interleukin-17A Triggers the Release of Platelet-Derived Factors Driving Vascular Endothelial Cells toward a Pro-Angiogenic State
    Authors: A Gatsiou, K Sopova, A Tselepis, K Stellos
    Cells, 2021-07-22;10(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-25 dampens the growth of human germinal center-derived B-cell non Hodgkin Lymphoma by curtailing neoangiogenesis
    Authors: E Ferretti, E Di Carlo, E Ognio, G Fraternali, A Corcione, B Belmonte, JL Ravetti, C Tripodo, D Ribatti, V Pistoia
    Oncoimmunology, 2017-11-27;7(3):e1397249.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. IL-17A Contributes to the Pathogenesis of Endometriosis by Triggering Proinflammatory Cytokines and Angiogenic Growth Factors.
    Authors: Ahn S, Edwards A, Singh S, Young S, Lessey B, Tayade C
    J Immunol, 2015-08-10;195(6):2591-600.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IL-17 Enhances Chemotaxis of Primary Human B Cells during Asthma.
    Authors: Halwani, Rabih, Al-Kufaidy, Roua, Vazquez-Tello, Alejandr, Pureza, Mary Ang, BaHammam, Ahmed S, Al-Jahdali, Hamdan, Alnassar, Sami A, Hamid, Qutayba, Al-Muhsen, Saleh
    PLoS ONE, 2014-12-10;9(12):e114604.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. T cell-derived IL-22 amplifies IL-1beta-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes.
    Authors: Dalmas E, Venteclef N, Caer C, Poitou C, Cremer I, Aron-Wisnewsky J, Lacroix-Desmazes S, Bayry J, Kaveri S, Clement K, Andre S, Guerre-Millo M
    Diabetes, 2014-02-11;63(6):1966-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
    Authors: Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E
    J. Immunol., 2009-11-01;183(9):5494-501.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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