Human IL-17RB Antibody

Catalog #: MAB12071 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB12071
MAB12071-SP

All IL-17RB Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-17RB Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑17 RB in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) IL-17 RB, recombinant human IL-17 R or rmIL-17 R is observed.

Source

Monoclonal Mouse IgG_2B Clone # 170216

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑17 RB
Arg18-Gly289
Accession # Q9NRM6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑17 RB Fc Chimera (Catalog # 1207-BR)

Human IL-17 RB Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human IL-17RB Biotinylated Antibody (Catalog # BAM1207)

Standard: Recombinant Human IL-17RB Fc Chimera Protein, CF (Catalog # 1207-BR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1). Interleukin-17 B Receptor (IL-17 RB), also known as IL-17Rh1, IL-17ER and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17 RB cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17 RB has also been identified (4). Human and mouse IIL-17 RB share 76% aa sequence identity. The human IL-17 RB protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17 RB is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17 RB is significantly up-regulated under inflammatory conditions.
IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2-4).

References

Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
Shi Y, et al. (2000) J. Biol. Chem. 275:19167.
Lee, J, et al. (2001) J. Biol. Chem. 276:1660.
Tian E, et al. (2000) Oncogene 19:2098.
Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
Hurst, S.D. et al. (2002) J. Immunol. 169:443.

Long Name

Interleukin 17B Receptor

Entrez Gene IDs

55540 (Human); 50905 (Mouse)

Alternate Names

CRL4IL-17 receptor homolog 1; cytokine receptor CRL4; Cytokine receptor-like 4; Evi27; EVI27IL-17B receptor; IL-17 RB; IL-17 receptor B; IL-17 RH1; IL17BRinterleukin 17B receptor; IL-17ER; IL17RB; IL-17RB; IL17Rh1; IL-17Rh1; IL17RH1MGC5245; interleukin 17 receptor B; interleukin 17 receptor homolog 1; interleukin-17 receptor B; Interleukin-17B receptor