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Human IL-17RC Antibody

Catalog #: AF2269
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2269
AF2269-SP
Detection of IL‑17 RC in K562 Human Cell Line by Flow Cytometry.
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Citations (5)
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Human IL-17RC Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17 RC in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse IL-17 RC and 5% cross-reactivity with recombinant human (rh) IL-17 R, rhIL-17 RD, and rhIL‑17B R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RC
Leu21-Ala454
Accession # NP_116121
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑17 RC (Catalog # 2269-IL)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-17 RC antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑17 RC in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human IL-17 RC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RC

IL-17 receptor C (IL-17 RC; also known as IL-17 RL and IL-17 Rhom) is an 85-110 kDa member of the IL-17 receptor family. This is one of five families that currently comprise the cytokine receptor superfamily (1-6). At this time, there are five members within the IL-17 receptor family, and these are termed IL-17 RA, B, C, D, and E. Not all receptors appear to bind known members of the IL-17 cytokine family. To date, IL-17 RA is reported to bind IL-17(A), while IL-17 RB is reported to bind IL‑17B and IL-17E (2, 4). Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. These include endothelial cells (6, 7), chondrocytes and osteoblasts (8), breast and prostatic epithelium (6), and fibroblasts, plus renal tubular epithelium and skeletal muscle cells (8, 9). Full-length IL-17 RC is synthesized as a 791 amino acid (aa) precursor (10, 11). It contains a 20 aa signal sequence, a 518 aa extracellular domain (ECD) (aa 21‑538), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region. Although IL-17 RA has two fibrinogen-like regions in its ECD that contribute to its function, no such architecture has been identified in the ECD of IL-17 RC (12). Based on studies looking at exon deletions, a key ligand-binding site would appear to exist over aa 425-441 (13). The gene for human IL-17 RC contains 19 exons. It is estimated that there are over 90 alternative splice forms, with transmembrane‑containing isoforms predominating (6, 14). The full-length isoform is estimated to occur approximately 10% of the time, while the three most common isoforms, as a group, occur about 50% of the time. Based on limited information, alternative splicing appears to regulate ligand specificity (13). R&D Systems IL‑17 RC corresponds to IL-17 RC isoform # 3, which shows deletions of aa 36-106 and 264-278 relative to the full-length form (10). Over the ECD, IL‑17 RC isoform #3 is 68% aa identical to mouse IL‑17 RC ECD. IL-17 RC is the cognate receptor for IL-17F, and binds IL-17A with similar affinity (13). With IL‑17 RA, it forms a definitive receptor for both IL-17A and IL-17F. The stoichiometry is unclear; it may form a heterodimer with IL-17 RA, or a heterotrimer with a preexisting IL‑17 RA homodimer (4, 9, 13, 15). The heteromeric nature of the receptor may be important given that the predominant form of the IL-17 cytokine is now considered to be an IL-17A:IL-17F heterodimer (4).

References
  1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
  2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
  3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  4. Shen, F. and S.L. Gaffen (2008) Cytokine 41:92.
  5. You, Z. et al. (2006) Cancer Res. 66:175.
  6. You, Z. et al. (2007) Neoplasia 9:464.
  7. Gerritsen, M.E. et al. (2003) Br. J. Pharmacol. 140:595.
  8. Kokubu, T. et al. (2008) J. Hictochem. Cytochem. 56:89.
  9. Toy, D. et al. (2006) J. Immunol. 177:36.
  10. GenBank Accession # Q96F46.
  11. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  12. Kramer, J.M. et al. (2007) J. Immunol. 179:6379.
  13. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  14. Haudenschild, D.R. et al. (2006) Prostate 66:1268.
  15. Kramer, J.M. et al. (2006) J. Immunol. 176:711.
Long Name
Interleukin 17 Receptor C, Transcript Variant 3
Entrez Gene IDs
84818 (Human); 171095 (Mouse)
Alternate Names
FLJ95963; FLJ96005; IL-17 RC; IL-17 receptor C; IL-17 RL; IL17F receptor; IL17RC; IL-17RC; IL17Rhom; IL17RL; IL-17RL; IL17-RL; interleukin 17 receptor C; interleukin-17 receptor C; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; MGC10763; zcytoR14

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Citations for Human IL-17RC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Defect of IL17 Signaling, but Not Centrinone, Inhibits the Development of Psoriasis and Skin Papilloma in Mouse Models
    Authors: B Jin, Y Zhang, HD Miller, L He, D Ge, AR Wang, Z You
    Biomedicines, 2022-08-15;10(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
    Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
    Eur J Immunol, 2015-12-14;46(2):440-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.
    Authors: Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM
    J. Immunol., 2008-08-15;181(4):2799-805.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
    Authors: Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P
    J. Immunol., 2008-01-01;180(1):655-63.
    Species: Human
    Sample Types: RNA, Whole Tissue
    Applications: IHC-P, Western Blot
  5. Expression of interleukin-17RC protein in normal human tissues
    Authors: Dongxia Ge, Zongbing You
    International Archives of Medicine

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