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Human IL-18/IL-1F4 Antibody

Catalog #: D044-3
27 Citations Datasheet / COA / SDS
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D044-3
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Citations (27)
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Human IL-18/IL-1F4 Antibody Summary

Species Reactivity
Human
Specificity
This antibody reacts with human IL-18 on Immunoprecipitation.
Source
Monoclonal Mouse IgG1 Clone # 125-2H
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human IL-18/IL-1F4
Formulation
100 μg IgG in 100 μL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunoprecipitation

5 μg/0.5 μg recombinant human IL-18

 

Human IL-18/IL-1F4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL‑18/IL‑1F4 Biotinylated Antibody (Catalog # D045-6)

Standard: Recombinant Human IL-18/IL-1F4 Protein (Catalog # B001-5)

Neutralization
Induction of IFN-gamma by KG-1 cell (Human myelomonocyte: ATCC CCL246) in response to the 40 ng/mL recombinant Human IL-18 was neutralized by this antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody solution is stable for one year from the date of purchase when stored at -20°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References
  1. Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)
  2. Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)
  3. Wu, C., J. Immunol. 170, 5571-5577 (2003)
  4. Sugawara, S., J. Immunol. 167, 6568-6575 (2001)
  5. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
  6. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  7. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  8. Okamura, H., et al., Nature 378, 88-91 (1995)
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

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Citations for Human IL-18/IL-1F4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Interleukin-18 enhances IL-18R/Nox1 binding, and mediates TRAF3IP2-dependent smooth muscle cell migration. Inhibition by simvastatin.
    Authors: Valente AJ, Yoshida T, Izadpanah R et al.
    Cell Signal.
  2. Protective heterologous T cell immunity in COVID-19 induced by the trivalent MMR and Tdap vaccine antigens
    Authors: Vijayashree Mysore, Xavier Cullere, Matthew L. Settles, Xinge Ji, Michael W. Kattan, Michaël Desjardins et al.
    Med (N Y)
  3. cAbl Kinase Regulates Inflammasome Activation and Pyroptosis via ASC Phosphorylation
    Authors: Mikhail A. Gavrilin, Evan R. Prather, Alex D. Vompe, Christian C. McAndrew, Mark D. Wewers
    The Journal of Immunology
  4. Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells
    Authors: LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith
    Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4652-4660.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. MR1-Independent Activation of Human Mucosal-Associated Invariant T Cells by Mycobacteria
    Authors: S Suliman, M Murphy, M Musvosvi, A Gela, EW Meermeier, H Geldenhuys, C Hopley, A Toefy, N Bilek, A Veldsman, WA Hanekom, JL Johnson, WH Boom, G Obermoser, H Huang, M Hatherill, DM Lewinsohn, E Nemes, TJ Scriba
    J. Immunol., 2019-10-14;0(0):.
    Species: Human
    Sample Types: Whole Blood
    Applications: Neutralization
  6. Aspergillus�fumigatus enhances human NK cell activity by regulating M1 macrophage polarization
    Authors: X Zhang, D He, S Gao, Y Wei, L Wang
    Mol Med Rep, 2019-06-06;20(2):1241-1249.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j
    Authors: SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner
    J. Immunol., 2017-12-27;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  8. IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD
    Authors: E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch
    Respir. Res., 2017-08-22;18(1):159.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  9. Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
    Authors: LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton
    Cytokine, 2017-07-13;0(0):.
    Species: Human
    Sample Types: Tissue Culture Supernates
    Applications: ELISA Development (Capture)
  10. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
    Sci Rep, 2016-09-02;6(0):32320.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  11. Bacillus Calmette-Gu�rin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses
    J Immunol, 2016-07-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection
    Authors: Martin R Goodier
    J Immunol, 2016-05-27;197(1):313-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
    Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
    J Immunol, 2015-07-31;195(5):2141-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  14. Type I IFNs and IL-18 regulate the antiviral response of primary human gammadelta T cells against dendritic cells infected with Dengue virus.
    Authors: Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, Macary P, Leo Y, Wong S, Puan K, Wong S
    J Immunol, 2015-03-02;194(8):3890-900.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways.
    Authors: Hedl M, Zheng S, Abraham C
    J Immunol, 2014-05-19;192(12):5924-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1.
    Authors: Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J
    Cytokine, 2013-11-23;65(2):222-30.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  17. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  18. Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.
    Authors: Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2009-06-26;297(2):H874-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  20. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  21. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
    Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
    Arthritis Rheum., 2006-08-01;54(8):2402-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.
    Authors: Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC
    J. Immunol., 2006-05-01;176(9):5284-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. The proatherogenic cytokine interleukin-18 is secreted by human adipocytes.
    Authors: Skurk T, Kolb H, Muller-Scholze S, Rohrig K, Hauner H, Herder C
    Eur. J. Endocrinol., 2005-06-01;152(6):863-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
    Authors: Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T
    J. Immunol., 2003-06-01;170(11):5571-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay, Neutralization
  25. Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells
    Authors: J Liu, G Song, X Lin, X Pang, T Meng
    Exp Ther Med, 2017-02-21;13(4):1447-1455.
  26. Outcome of SARS-CoV-2 infection is linked to MAIT cell activation and cytotoxicity
    Authors: H Flament, M Rouland, L Beaudoin, A Toubal, L Bertrand, S Lebourgeoi, C Rousseau, P Soulard, Z Gouda, L Cagninacci, AC Monteiro, M Hurtado-Ne, S Luce, K Bailly, M Andrieu, B Saintpierr, F Letourneur, Y Jouan, M Si-Tahar, T Baranek, C Paget, C Boitard, A Vallet-Pic, JF Gautier, N Ajzenberg, B Terrier, F Pène, J Ghosn, X Lescure, Y Yazdanpana, B Visseaux, D Descamps, JF Timsit, RC Monteiro, A Lehuen
    Nature Immunology, 2021-02-02;0(0):.
  27. Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients
    Authors: F Magnotti, L Lefeuvre, S Benezech, T Malsot, L Waeckel, A Martin, S Kerever, D Chirita, M Desjonquer, A Duquesne, M Gerfaud-Va, A Laurent, P Sève, MR Popoff, T Walzer, A Belot, Y Jamilloux, T Henry
    EMBO Mol Med, 2019-10-07;0(0):e10547.

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