Human IL-18/IL-1F4 Propeptide PE-conjugated Antibody Summary
Accession # Q14116
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑18/IL‑1F4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (upper panel) or treated with LPS for 24 hours (lower panel) were stained with Mouse Anti-Human IL-18/IL-1F4 PE-conjugated Monoclonal Antibody (Catalog # IC646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of IL‑18/IL‑1F4 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human monocytic leukemia cell line was stained with Mouse Anti-Human IL-18/IL-1F4 PE-conjugated Monoclonal Antibody (Catalog # IC646P, filled histogram) or isotype control antibody (IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-18/IL-1F4
Pro-IL-18 (pro-Interleukin 18; also known as pro-IGIF and pro-IL-1 gamma ) is a 24 kDa member of the IL-1 family of molecules. It is widely expressed, being produced by keratinocytes, intestinal epithelium, T cells, macrophages and osteoblasts. Human Pro-IL-18 is 193 amino acids (aa) in length. Although mature IL-18 induces IFN-gamma secretion by NK and T cells, Pro-IL-18 appears to have little intrinsic activity. Generally, active IL-18 is considered to arise from caspase-1 cleavage of Pro-IL-18 between Asp36-Tyr37. This generates an 18 kDa mature C-terminal fragment, and a 4 kDa (predicted) N-terminal prosegment that runs at 6 kDa in SDS-PAGE. Other proteases are known to process Pro-IL-18. Caspase-3 cleavage after Asp68 generates an inactive 14 kDa mature segment, Merpin beta -subunit cleavage after Asn52 generates a marginally active 17 kDa mature segment, while parasite Cys protease cleavage after Val47 generates an inactive 17 kDa mature molecule. One splice variant shows a deletion of aa 27-30. Over aa 2-36, human Pro-IL-18 shares 63% aa identity with mouse Pro-IL-18.
Product Datasheets
Citations for Human IL-18/IL-1F4 Propeptide PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Interleukin-35 suppresses the activity of natural killer-like B cells in patients with hepatocellular carcinoma
Authors: S Liu, L Yang, S Jia, R Zhao, Z Jin
International immunopharmacology, 2021-09-20;100(0):108161.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Altered expression of IL-18 binding protein and IL-18 receptor in basophils and mast cells of asthma patients
Authors: Z Wang, Z Liu, L Wang, J Wang, L Chen, H Xie, H Zhang, S He
Scand. J. Immunol., 2018-04-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human CD8(+) EMRA T cells display a senescence-associated secretory phenotype regulated by p38 MAPK
Authors: LA Callender, EC Carroll, RWJ Beal, ES Chambers, S Nourshargh, AN Akbar, SM Henson
Aging Cell, 2017-10-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R
Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He
J. Cell. Mol. Med., 2017-09-18;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells
Authors: Y Hu, J Wang, H Zhang, H Xie, W Song, Q Jiang, N Zhao, S He
Mediators Inflamm., 2017-08-03;2017(0):3090782.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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