Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human IL-19 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
12.2 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant human IL-19
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human IL-19 immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-19 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human IL-19 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-19 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-19.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 292 | 805 | 1586 | 269 | 739 | 1478 |
| Standard Deviation | 10.3 | 19 | 41.8 | 22 | 56.8 | 103 |
| CV% | 3.5 | 2.4 | 2.6 | 8.2 | 7.7 | 7 |
Recovery
The recovery of human IL-19 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 93-104 |
| EDTA Plasma (n=4) | 94 | 85-102 |
| Heparin Plasma (n=4) | 93 | 87-99 |
| Serum (n=4) | 93 | 87-97 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-19 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-19
Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. IL-19 contains seven helices and is secreted as a 35 kDa glycosylated monomer. IL-19 expression has been observed in keratinocytes and monocytes. IL-19 binds a receptor complex consisting of IL-20R alpha and the IL-20 R beta. This receptor complex is also shared by IL-20 and IL-24.
Long Name:
Interleukin 19
Entrez Gene IDs:
29949 (Human); 329244 (Mouse)
Alternate Names:
IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human IL-19 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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Interleukin-19 Levels Are Increased in Palmoplantar Pustulosis and Reduced following Apremilast Treatment
Authors: K Wolk, D Wilsmann-T, K Witte, TC Brembach, C Kromer, S Gerdes, K Ghoreschi, K Reich, R Mössner, R Sabat
International Journal of Molecular Sciences, 2023-01-09;24(2):.
Species: Human
Sample Types: Plasma
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The Mediating Effect of Cytokines on the Association between Fungal Sensitization and Poor Clinical Outcome in Asthma
Authors: CH Lin, YR Li, CT Kor, SH Lin, BC Ji, MT Lin, WH Chai
Biomedicines, 2022-06-19;10(6):.
Species: Human
Sample Types: Serum
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Novel gene variants associated with cardiovascular disease in systemic lupus erythematosus and rheumatoid arthritis
Authors: D Leonard, E Svenungsso, J Dahlqvist, A Alexsson, L Ärlestig, KE Taylor, JK Sandling, C Bengtsson, M Frodlund, A Jönsen, S Eketjäll, K Jensen-Urs, I Gunnarsson, C Sjöwall, AA Bengtsson, ML Eloranta, AC Syvänen, S Rantapää-D, LA Criswell, L Rönnblom
Ann. Rheum. Dis., 2018-03-07;0(0):.
Species: Human
Sample Types: Serum
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The Stimulation of Macrophages with TLR Ligands Supports Increased IL-19 Expression in Inflammatory Bowel Disease Patients and in Colitis Models
Authors: A Steinert, I Linas, B Kaya, M Ibrahim, A Schlitzer, P Hruz, K Radulovic, L Terraccian, AJ Macpherson, JH Niess
J. Immunol., 2017-09-01;0(0):.
Species: Human
Sample Types: Serum
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Interleukin-19 impairment in active Crohn's disease patients.
Authors: Canto E, Garcia Planella E, Zamora-Atenza C, Nieto J, Gordillo J, Ortiz M, Meton I, Serrano E, Vegas E, Garcia-Bosch O, Juarez C, Vidal S
PLoS ONE, 2014-04-09;9(4):e93910.
Species: Human
Sample Types: Serum
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The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
Eur. J. Immunol., 2009-12-01;39(12):3570-81.
Species: Human
Sample Types: Serum
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Crimean Congo hemorrhagic fever virus infects human monocyte-derived dendritic cells.
Authors: Connolly-Andersen AM, Douagi I, Kraus AA, Mirazimi A
Virology, 2009-06-30;390(2):157-62.
Species: Human
Sample Types: Cell Culture Supernates
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Human IL-19 Quantikine ELISA Kit
By Anonymous
on 08/06/2018
Sample Tested: Serum
