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Human IL-1 alpha /IL-1F1 Antibody

Catalog #: MAB200
30 Citations 1 Review Datasheet / COA / SDS
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MAB200-SP
MAB200-100
MAB200-500
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Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody.
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Human IL-1 alpha /IL-1F1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 alpha /IL-1F1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta, -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha, -1 beta, -3, -4, -5, -6, -7, -9, or -13.
Source
Monoclonal Mouse IgG2A Clone # 4414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-1 alpha /IL-1F1
Ser113-Ala271
Accession # P01583
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA)
Immunocytochemistry
8-25 µg/mL
See below

Human IL-1 alpha /IL-1F1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL‑1 alpha /IL‑1F1 Biotinylated Antibody (Catalog # BAF200)

Standard: Recombinant Human IL-1 alpha/IL-1F1 Protein (Catalog # 200-LA)

Neutralization
Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 0.05 ng/mL Recombinant Human IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody. View Larger

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody. Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB200). The ND50 is typically 0.05-0.15 µg/mL.

Immunocytochemistry IL‑1a/IL‑1F1 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑1 alpha /IL‑1F1 in Human PBMCs. IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Human IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB200) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts, and glia (oligodendroglia, astrocytes, and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

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Citations for Human IL-1 alpha /IL-1F1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Glucose Induces IL-1 alpha -Dependent Inflammation and Extracellular Matrix Proteins Expression and Deposition in Renal Tubular Epithelial Cells in Diabetic Kidney Disease
    Authors: Talal Salti, Khaled Khazim, Rami Haddad, Salvatore Campisi-Pinto, Gil Bar-Sela, Idan Cohen
    Frontiers in Immunology
  2. Staphylococcus aureus stimulates neutrophil targeting chemokine expression in keratinocytes through an autocrine IL-1alpha signaling loop
    Authors: Florina Olaru, Liselotte E. Jensen
    Journal of Investigative Dermatology
  3. IL-1R1 Signaling Facilitates Munro’s Microabscess Formation in Psoriasiform Imiquimod-Induced Skin Inflammation
    Authors: Mireia Uribe-Herranz, Li-Hua Lian, Kirsten M. Hooper, Katelynn A. Milora, Liselotte E. Jensen
    Journal of Investigative Dermatology
  4. MicroRNAs miR-146a/b negatively modulate the senescence-associated inflammatory mediators IL-6 and IL-8
    Authors: Dipa Bhaumik, Gary K. Scott, Shiruyeh Schokrpur, Christopher K. Patil, Arturo V. Orjalo, Francis Rodier et al.
    ImpactJ
  5. Myocardial Accumulations of Reg3A, Reg3 gamma and Oncostatin M Are Associated with the Formation of Granulomata in Patients with Cardiac Sarcoidosis
    Authors: Praveen Gajawada, Ayse Cetinkaya, Susanne von Gerlach, Natalia Kubin, Heiko Burger, Michael Näbauer et al.
    International Journal of Molecular Sciences
  6. Molecule-Resolved Visualization of Particulate Matter on Human Skin Using Multimodal Nonlinear Optical Imaging
    Authors: ES Lee, S Kim, SW Lee, J Jung, SH Lee, HW Na, HJ Kim, YD Hong, WS Park, TG Lee, DG Jo, SH Kim
    International Journal of Molecular Sciences, 2021-05-14;22(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. COX2 regulates senescence secretome composition and senescence surveillance through PGE2
    Authors: S Gonçalves, K Yin, Y Ito, A Chan, I Olan, S Gough, L Cassidy, E Serrao, S Smith, A Young, M Narita, M Hoare
    Cell Reports, 2021-03-16;34(11):108860.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  8. Characterization of the TNF and IL-1 systems in human brain and blood after ischemic stroke
    Authors: BH Clausen, M Wirenfeldt, SS Høgedal, LH Frich, HH Nielsen, HD Schrøder, K Østergaard, B Finsen, BW Kristensen, KL Lambertsen
    Acta Neuropathol Commun, 2020-06-05;8(1):81.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
    Authors: CM Henry, SJ Martin
    Mol. Cell, 2017-02-16;65(4):715-729.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.
    Authors: Marina Beltrami-M, Amélie Vromman, Galina K Sukhova, Eduardo J Folco, Peter Libby
    PLoS ONE, 2016-03-31;0(0):1932-6203.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation.
    Authors: Horbelt D, Boergermann J, Chaikuad A, Alfano I, Williams E, Lukonin I, Timmel T, Bullock A, Knaus P
    J Biol Chem, 2014-11-03;290(6):3390-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Redox control of the senescence regulator interleukin-1alpha and the secretory phenotype.
    Authors: McCarthy D, Clark R, Bartling T, Trebak M, Melendez J
    J Biol Chem, 2013-09-23;288(45):32149-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 2011-12-21;5(2):140-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Protection from RNA and DNA viruses by IL-32.
    Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
    J. Immunol., 2011-02-23;186(7):4110-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  15. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
    Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
    Int. J. Cancer, 2010-07-15;127(2):332-44.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  16. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  17. Ectopic expression of interleukin-1 receptor type II enhances cell migration through activation of the pre-interleukin 1alpha pathway.
    Authors: Chang SY, Su PF, Lee TC
    Cytokine, 2008-11-20;45(1):32-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008-07-01;181(1):557-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  19. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  20. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  21. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007-12-01;117(12):3664-3672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
    Authors: Rao DA, Tracey KJ, Pober JS
    J. Immunol., 2007-11-15;179(10):6536-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  24. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  25. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  26. Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway.
    Authors: Lee KY, Ho SC, Lin HC, Lin SM, Liu CY, Huang CD, Wang CH, Chung KF, Kuo HP
    Am. J. Respir. Cell Mol. Biol., 2006-05-11;35(4):407-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment
    Authors: Mirella Georgouli, Cecilia Herraiz, Eva Crosas-Molist, Bruce Fanshawe, Oscar Maiques, Anna Perdrix et al.
    Cell
  28. Senescence-associated secretory factors induced by cisplatin in melanoma cells promote non-senescent melanoma cell growth through activation of the ERK1/2-RSK1 pathway
    Authors: X Sun, B Shi, H Zheng, L Min, J Yang, X Li, X Liao, W Huang, M Zhang, S Xu, Z Zhu, H Cui, X Liu
    Cell Death Dis, 2018-02-15;9(3):260.
  29. Sterile inflammation of endothelial cell-derived apoptotic bodies is mediated by interleukin-1 alpha
    Authors: Yaël Berda-Haddad, Stéphane Robert, Paul Salers, Leila Zekraoui, Catherine Farnarier, Charles A. Dinarello et al.
    Proceedings of the National Academy of Sciences
  30. Regulation of CD137 expression through K-Ras signaling in pancreatic cancer cells
    Authors: Christophe Glorieux, Peng Huang
    Cancer Communications

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Human IL-1 alpha /IL-1F1 Antibody
By ‪Idan Cohen‬‏ on 07/13/2022
Application: IHC Sample Tested: Adult kidney Species: Human
Immunohistochemistry Human IL-1 alpha /IL-1F1 Antibody MAB200