Human IL‑21R Subunit Biotinylated Antibody

Name: Human IL‑21R Subunit Biotinylated Antibody
Brand: R&D Systems
SKU: BAF991
Rating: 5 (1 reviews)

Catalog #: BAF991
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF991

All IL-21R Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL‑21R Subunit Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-21 R Subunit in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse IL‑21 R Subunit is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 R Subunit
Cys20-Pro236
Accession # Q9HBE5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑21 R Subunit Fc Chimera (Catalog # 991-R2)

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood CD19⁺ B cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21R

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha, and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain, and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B, and NK lineage. The common gamma chain ( gamma _c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

References

Parrish-Novak, et al. (2000) Nature 408:57.
Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
Asao, H. et al. (2001) J. Immunol. 167:1.

Long Name

Interleukin 21 Receptor

Entrez Gene IDs

50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)

Alternate Names

CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human IL‑21R Subunit Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

IL-21 sustains CD28 expression on IL-15-activated human naive CD8+ T cells.
Authors: Alves NL, Arosa FA, van Lier RA
J. Immunol., 2005-07-15;175(2):755-62.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry