Human IL-22 Antibody

Detection of Human IL‑22 by Western Blot. Western blot shows lysates of human tonsil tissue and human breast cancer tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-22 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑22 in Human PBMCs. IL-22 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS) using 10 µg/mL Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL‑10 Secretion Induced by IL‑22 and Neutralization by Human IL‑22 Antibody. Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782). The ND₅₀ is typically 0.5-2.5 µg/mL.

Detection of Human IL-22 by Western Blot Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.(A) IL-22 positive cells mainly located in portal areas with brownish-yellow color by immunohistochemistry. (B) Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue. Black arrows indicate double-positive cells. The frequencies of IL-22 cells (C) and the expression of IL-22 mRNA (D) in the liver tissue were significantly higher in HCV-OLT patients, compared to the OLT and HCV patients. (E) Protein in the liver also had the same change by Western-blotting. IL-22 cells are positively correlated with alpha -SMA (F) and fibrosis staging (G), not with grading score (H) and HCVRNA (I). *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001, ns p>0.05. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0154419), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human IL-22 by Western Blot Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.(A) IL-22 positive cells mainly located in portal areas with brownish-yellow color by immunohistochemistry. (B) Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue. Black arrows indicate double-positive cells. The frequencies of IL-22 cells (C) and the expression of IL-22 mRNA (D) in the liver tissue were significantly higher in HCV-OLT patients, compared to the OLT and HCV patients. (E) Protein in the liver also had the same change by Western-blotting. IL-22 cells are positively correlated with alpha -SMA (F) and fibrosis staging (G), not with grading score (H) and HCVRNA (I). *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001, ns p>0.05. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0154419), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Human IL-22 Antibody by Immunohistochemistry IL-22 and IL-22R1 are expressed in human brain. IL-22, IL-22R1, GFAP, and Caveolin-1 immunohistochemistry peroxidase stainings of brain tissue sections of control (a) and MS (b–d) patients. MOG and HE stainings were used to detect MS demyelinating plaques (d). All four pictures belonging to one column (a, b, c, or d) were always immediately adjacent to each other. Pictures a, b, and c were taken at areas at the border between GM and NAWM, whereas pictures in d were taken from the same location at the edge between NAWM and a plaque. Inserts in columns a and b represent a threefold magnification of the selected area. Arrow: astrocyte-like pattern. a study patient B-C2, b and d study patient B-MS3, c study patient B-MS5 (Table 2). Scale bar, 50 μm (a, b: ×20, c, d ×40). GM: gray matter, NAWM: normal appearing white matter, WM: white matter. Representative pictures obtained from the observations of seven control and five MS autopsy samples Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26077779), licensed under a CC-BY license. Not internally tested by R&D Systems.