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Human IL-22 PE-conjugated Antibody

Catalog #: IC7821P
37 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC7821P-100
IC7821P-025
Detection of IL‑22 in Human PBMCs by Flow Cytometry.
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Citations (37)
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Human IL-22 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.
Source
Monoclonal Mouse IgG1 Clone # 142928
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-22
Ala34-Ile179
Accession # Q9GZX6
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-22 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑22 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Ca++Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human IL-22 PE-conjugated Monoclonal Antibody (Catalog # IC7821P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

References
  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Long Name
Interleukin 22
Entrez Gene IDs
50616 (Human); 50929 (Mouse); 500836 (Rat)
Alternate Names
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18

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Citations for Human IL-22 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. IL‑22/IL‑22R1 promotes proliferation and collagen synthesis of MRC‑5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis
    Authors: Juan Liu, Biao Shang, Jing Bai
    Experimental and Therapeutic Medicine
  2. Alterations of the intestinal mucus layer correlate with dysbiosis and immune dysregulation in human Type 1 Diabetes
    Authors: M Lo Conte, I Cosorich, R Ferrarese, M Antonini C, A Nobili, V Palmieri, L Massimino, LA Lamparelli, W Liang, M Riba, E Devecchi, AM Bolla, E Pedone, M Scavini, E Bosi, A Fasano, F Ungaro, J Diana, N Mancini, M Falcone
    EBioMedicine, 2023-04-14;91(0):104567.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature
    Authors: Miqdad O. Dhariwala, Dhuvarakesh Karthikeyan, Kimberly S. Vasquez, Sepideh Farhat, Antonin Weckel, Keyon Taravati et al.
    Cell Reports Medicine
  4. Circulating CD56bright NK cells inversely correlate with survival of melanoma patients
    Authors: Kaat de Jonge, Anna Ebering, Sina Nassiri, Hélène Maby-El Hajjami, Hajer Ouertatani-Sakouhi, Petra Baumgaertner et al.
    Scientific Reports
  5. RNA-seq and flow-cytometry of conventional, scalp, and palmoplantar psoriasis reveal shared and distinct molecular pathways
    Authors: R Ahn, D Yan, HW Chang, K Lee, S Bhattarai, ZM Huang, M Nakamura, R Singh, L Afifi, K Taravati, P Munoz-Sand, M Pauli, MD Rosenblum, W Liao
    Sci Rep, 2018-07-27;8(1):11368.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Genetic deficiency of NOD2 confers resistance to invasive aspergillosis
    Authors: MS Gresnigt, C Cunha, M Jaeger, SM Gonçalves, RKS Malireddi, A Ammerdorff, R Lubbers, M Oosting, O Rasid, G Jouvion, C Fitting, DJ Jong, JF Lacerda, A Campos, WJG Melchers, K Lagrou, J Maertens, TD Kanneganti, A Carvalho, O Ibrahim-Gr, FL van de Vee
    Nat Commun, 2018-07-06;9(1):2636.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine
    Authors: N Li, V van Unen, T Höllt, A Thompson, J van Bergen, N Pezzotti, E Eisemann, A Vilanova, SM Chuva de S, BPF Lelieveldt, F Koning
    J. Exp. Med., 2018-03-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A20 upregulation during treated HIV disease is associated with intestinal epithelial cell recovery and function
    Authors: AS Chitre, MG Kattah, YY Rosli, M Pao, M Deswal, SG Deeks, PW Hunt, M Abdel-Mohs, LJ Montaner, CC Kim, A Ma, M Somsouk, JM McCune
    PLoS Pathog., 2018-03-05;14(3):e1006806.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Sequential inflammatory processes define human progression from M. tuberculosis infection to tuberculosis disease
    Authors: Thomas J. Scriba, Adam Penn-Nicholson, Smitha Shankar, Tom Hraha, Ethan G. Thompson, David Sterling et al.
    PLOS Pathogens
  10. High frequency of intestinal TH17 cells correlates with microbiota alterations and disease activity in multiple sclerosis
    Authors: I Cosorich, G Dalla-Cost, C Sorini, R Ferrarese, MJ Messina, J Dolpady, E Radice, A Mariani, PA Testoni, F Canducci, G Comi, V Martinelli, M Falcone
    Sci Adv, 2017-07-12;3(7):e1700492.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Composition and functionality of the intrahepatic innate lymphoid cell-compartment in human non-fibrotic and fibrotic livers
    Authors: M Forkel, L Berglin, E Kekäläinen, A Carlsson, E Svedin, J Michaëlsso, M Nagasawa, JS Erjefält, M Mori, M Flodström-, A Bergquist, HG Ljunggren, M Westgren, U Lindforss, D Friberg, C Jorns, E Ellis, NK Björkström, J Mjösberg
    Eur. J. Immunol., 2017-07-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Skin-infiltrating, interleukin-22-producing T cells differentiate pediatric psoriasis from adult psoriasis
    Authors: KM Cordoro, M Hitraya-Lo, K Taravati, PM Sandoval, E Kim, J Sugarman, ML Pauli, W Liao, MD Rosenblum
    J. Am. Acad. Dermatol., 2017-06-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Enhancing adoptive cancer immunotherapy with V?2V?2 T cells through pulse zoledronate stimulation
    Authors: MH Nada, H Wang, G Workalemah, Y Tanaka, CT Morita
    J Immunother Cancer, 2017-02-21;5(0):9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. IL-22-producing CD4+T cells in the treatment response of rheumatoid arthritis to combination therapy with methotrexate and leflunomide
    Authors: W Zhong, L Zhao, T Liu, Z Jiang
    Sci Rep, 2017-01-24;7(0):41143.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Expansion of Th17 Cells by Human Mast Cells Is Driven by Inflammasome-Independent IL-1?
    Authors: J Suurmond, KL Habets, AL Dorjée, TW Huizinga, RE Toes
    J. Immunol, 2016-10-31;197(11):4473-4481.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
    Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
    J Exp Med, 2015-04-27;212(5):619-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination.
    Authors: Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z
    Eur J Immunol, 2015-02-01;45(2):442-51.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. The effect of aging on the frequency, phenotype and cytokine production of human blood CD4 + CXCR5 + T follicular helper cells: comparison of aged and young subjects.
    Authors: Zhou M, Zou R, Gan H, Liang Z, Li F, Lin T, Luo Y, Cai X, He F, Shen E
    Immun Ageing, 2014-08-23;11(0):12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. CX(3)CR1(+) mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22.
    Authors: Longman R, Diehl G, Victorio D, Huh J, Galan C, Miraldi E, Swaminath A, Bonneau R, Scherl E, Littman D
    J Exp Med, 2014-07-14;211(8):1571-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
    Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
    J Invest Dermatol, 2014-03-21;134(9):2351-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. A higher frequency of circulating IL-22(+)CD4(+) T cells in Chinese patients with newly diagnosed Hashimoto's thyroiditis.
    Authors: Guo H, Peng D, Yang X, Wang Y, Xu B, Ni J, Meng W, Jiang Y
    PLoS ONE, 2014-01-03;9(1):e84545.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis.
    Authors: Gironi, Maira, Saresella, Marina, Rovaris, Marco, Vaghi, Matilde, Nemni, Raffaell, Clerici, Mario, Grossi, Enzo
    Immun Ageing, 2013-01-10;10(1):1.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012-09-25;10(9):e1001395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Functionally distinct subsets of human FOXP3+ Treg cells that phenotypically mirror effector Th cells.
    Authors: Duhen T, Duhen R, Lanzavecchia A
    Blood, 2012-03-21;119(19):4430-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011-11-02;17(24):7529-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Gain-of-function human STAT1 mutations impair IL-17 immunity and underlie chronic mucocutaneous candidiasis
    Authors: Luyan Liu, Satoshi Okada, Xiao-Fei Kong, Alexandra Y. Kreins, Sophie Cypowyj, Avinash Abhyankar et al.
    Journal of Experimental Medicine
  27. ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection.
    Authors: Qiao D, Yang BY, Li L, Ma JJ, Zhang XL, Lao SH, Wu CY
    Scand. J. Immunol., 2011-04-01;73(4):330-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription.
    Authors: Liu Y, Yang B, Ma J, Wang H, Huang F, Zhang J, Chen H, Wu C
    Immunology, 2011-01-07;132(4):540-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
    Authors: Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB
    PLoS ONE, 2010-11-24;5(11):e14108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
    Authors: Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C
    Eur. J. Immunol., 2010-09-01;40(9):2450-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines.
    Authors: Kisand K, Boe Wolff AS, Podkrajsek KT, Tserel L, Link M, Kisand KV, Ersvaer E, Perheentupa J, Erichsen MM, Bratanic N, Meloni A, Cetani F, Perniola R, Ergun-Longmire B, Maclaren N, Krohn KJ, Pura M, Schalke B, Strobel P, Leite MI, Battelino T, Husebye ES, Peterson P, Willcox N, Meager A
    J. Exp. Med., 2010-02-01;207(2):299-308.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
    Authors: Carbone T, Nasorri F, Pennino D
    J. Immunol., 2009-12-11;184(2):1102-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
    Authors: Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2009-07-05;10(8):857-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Stage 3 immature human natural killer cells found in secondary lymphoid tissue constitutively and selectively express the TH 17 cytokine interleukin-22.
    Authors: Hughes T, Becknell B, McClory S, Briercheck E, Freud AG, Zhang X, Mao H, Nuovo G, Yu J, Caligiuri MA
    Blood, 2009-02-24;113(17):4008-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
    Authors: Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR
    Arthritis Rheum., 2008-03-01;58(3):875-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007-08-05;8(9):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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