Human IL-23R Biotinylated Antibody

Catalog #: BAF1400
11 Citations Datasheet / COA / SDS

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BAF1400

All IL-23R Antibodies

Product Details

Citations (11)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-23R Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-23 R in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse IL-23 R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-23 R
Gly24-Ile353
Accession # Q5VWK5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑23 R Fc Chimera (Catalog # 1400-IR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain, and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential STAT-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% aa sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

References

Oppmann, B. et al. (2000) Immunity 13:715.
Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Parham, C. et al. (2002) J. Immunol. 168:5448.
Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Long Name

Interleukin 23 Receptor

Entrez Gene IDs

149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)

Alternate Names

IBD17; IL-23 R; IL-23 receptor; IL23R; IL-23R; interleukin 23 receptor; interleukin-23 receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-23R Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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The effect of inhaled inactived Mycobacterium phlei as a treatment for asthma
Authors: Moyu Ming, Chaoqian Li, Zhixi Luo, Shengqiu Lv, Qixiang Sun
Molecular Medicine Reports
Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction
Authors: J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss
The Journal of Biological Chemistry, 2021-10-09;0(0):101295.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Deciphering site 3 interactions of IL-12 and IL-23 with their cognate murine and human receptors
Authors: A Esch, A Masiarz, S Mossner, JM Moll, J Grötzinger, J Schröder, J Scheller, DM Floss
J. Biol. Chem., 2020-06-09;0(0):.
Species: Mouse
Sample Types: Transduced Whole Cells
Applications: ICC
Prognostic value of serum soluble interleukin-23 receptor and related T-helper 17 cell cytokines in non-small cell lung carcinoma
Authors: D Liu, S Xing, W Wang, X Huang, H Lin, Y Chen, K Lan, L Chen, F Luo, S Qin, R Liang, C Bai, J Xu, W Liu
Cancer Sci., 2020-03-04;111(4):1093-1102.
Species: Human
Sample Types:
Applications: ELISA (detection)
The human IL-23 receptor rs11209026 A allele promotes the expression of a soluble IL-23R-encoding mRNA species.
Authors: Yu R, Brazaitis J, Gallagher G
J Immunol, 2014-12-31;194(3):1062-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
The IL23R R381Q Gene Variant Protects against Immune-Mediated Diseases by Impairing IL-23-Induced Th17 Effector Response in Humans.
Authors: Di Meglio P, Di Cesare A, Laggner U
PLoS ONE, 2011-02-22;6(2):e17160.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells.
Authors: Yu RY, Gallagher G
J. Immunol., 2010-11-12;185(12):7302-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Regulating human Th17 cells via differential expression of IL-1 receptor.
Authors: Lee WW, Kang SW, Choi J
Blood, 2009-11-12;115(3):530-40.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
J. Exp. Med., 2009-03-09;206(3):535-48.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants.
Authors: de Paus RA, van de Wetering D, Van Dissel JT
Mol. Immunol., 2008-09-01;45(15):3889-95.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1 beta -dependent response of Th17 cells
Authors: Dong Hyun Kim, Hee Young Kim, Sunjung Cho, Su-Jin Yoo, Won-Ju Kim, Hye Ran Yeon et al.
eLife