Human IL-23R PE-conjugated Antibody Summary
Gly24-Ile354
Accession # Q5VWK5
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑23 R in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (A) cultured with plate-bound 5 μg/mL anti-Human CD3, 2 μg/mL anti-Human CD28 (AF-342-PB), 20 ng/mL Recombinant Human IL-2 (202-GMP), 10 ng/mL TGF-beta (100-B), 20 ng/mL Recombinant Human IL-23 (1290-IL), 40 ng/mL Recombinant Human IL-6 (206-IL), 10 ng/mL Recombinant Human IL-1 beta (201-LB), and 10 μg/mL anti-Human IFN-gamma (AF-285-NA) for 7 days to induce Th17 development or (B) resting, were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A) and Mouse Anti-Human IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB14001P). Quadrant markers were set based on Mouse IgG2B Isotype Control (IC0041P). Staining was performed using our Staining Membrane-associated Proteins protocol.
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Detection of IL‑23 R in HEK293 cell line transfected with human IL-23 R by Flow Cytometry. HEK293 human cell line transfected with human IL-23 R were stained with Mouse Anti-Human IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB14001P, filled histogram) or Mouse IgG2B Isotype Control (IC0041P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-23R
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 aa type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.
- Oppmann, B. et al. (2000) Immunity 13:715.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
- Parham, C. et al. (2002) J. Immunol. 168:5448.
- Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
- Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Product Datasheets
Citations for Human IL-23R PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
16
Citations: Showing 1 - 10
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Systemic frequencies of T helper 1 and T helper 17 cells in patients with age-related macular degeneration: A case-control study
Authors: Amardeep Singh, Yousif Subhi, Marie Krogh Nielsen, Mads Krüger Falk, Sara Maj Hyldig Matzen, Finn Sellebjerg et al.
Scientific Reports
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Umbilical Cord Mesenchymal Stromal/Stem Cells and Their Interplay with Th-17 Cell Response Pathway
Authors: Najar, M;Rahmani, S;Faour, WH;Alsabri, SG;Lombard, CA;Fayyad-Kazan, H;Sokal, EM;Merimi, M;Fahmi, H;
Cells
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
In Vitro Cellular and Molecular Interplay between Human Foreskin-Derived Mesenchymal Stromal/Stem Cells and the Th17 Cell Pathway
Authors: M Najar, M Merimi, WH Faour, CA Lombard, D Moussa Agh, Y Ouhaddi, EM Sokal, L Lagneaux, H Fahmi
Pharmaceutics, 2021-10-19;13(10):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients.
Authors: Samarani S, Sagala P, Jantchou P, Grimard G, Faure C, Deslandres C, Amre D, Ahmad A
Mediators Inflamm, 2020-02-19;2020(0):6401969.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of interleukin-23 in monocyte-derived dendritic cells of HBV-related acute-on-chronic liver failure and its correlation with the severity of liver damage
Authors: Suxia Bao
Clin Res Hepatol Gastroenterol, 2016-12-29;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity
Authors: Lars Fugger
Sci Transl Med, 2016-11-02;8(363):363ra149.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination.
Authors: Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z
Eur J Immunol, 2015-02-01;45(2):442-51.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
Eur J Immunol, 2015-01-19;45(4):1092-102.
Species: Human
Sample Types: Whole Cells
Applications: FACS -
IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
PLoS Biol, 2012-09-25;10(9):e1001395.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
Authors: Cocco C, Di Carlo E, Zupo S
Leukemia, 2011-12-23;26(6):1365-74.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification of human NK17/NK1 cells.
Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
PLoS ONE, 2011-10-21;6(10):e26780.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
J. Immunol., 2011-03-02;186(7):4175-82.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
Immunol. Lett., 2010-10-15;134(2):113-21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants.
Authors: de Paus RA, van de Wetering D, Van Dissel JT
Mol. Immunol., 2008-09-01;45(15):3889-95.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL23 induces IL23R recycling and amplifies innate receptor-induced signalling and cytokines in human macrophages, and the IBD-protective IL23R R381Q variant modulates these outcomes
Authors: Rui Sun, Matija Hedl, Clara Abraham
Gut
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Estrogen and progesterone decrease let-7f microRNA expression and increase IL-23/IL-23 receptor signaling and IL-17A production in patients with severe asthma
Authors: Dawn C. Newcomb, Jacqueline Yvonne Cephus, Madison G. Boswell, John M. Fahrenholz, Emily W. Langley, Amy S. Feldman et al.
Journal of Allergy and Clinical Immunology
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Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100
