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Human IL-26/AK155 Antibody

Catalog #: MAB13751
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB13751
MAB13751-SP
Detection of IL‑26/AK155 in Human PBMCs by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-26/AK155 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-26/AK155 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 510414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-26/AK155
Lys22-Gln171
Accession # Q9NPH9.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL‑26/AK155 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑26/AK155 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with PMA and Calcium ionomycin, then stained with Mouse Anti-Human IL-26/AK155 Monoclonal Antibody (Catalog # MAB13751, dark orange filled histogram) or isotype control (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-26/AK155

IL-26, also named AK155, belongs to the family of IL-10-related cytokines.

Long Name
Interleukin 26
Entrez Gene IDs
55801 (Human)
Alternate Names
AK155; AK155IL-26interleukin-26; IL26; IL-26; interleukin 26; Protein AK155

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Citations for Human IL-26/AK155 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Interleukin-26 (IL-26) is a novel anti-microbial peptide produced by T cells in response to staphylococcal enterotoxin
    Authors: Anders Woetmann, Morten Alhede, Sally Dabelsteen, Thomas Bjarnsholt, Morten Rybtke, Claudia Nastasi et al.
    Oncotarget
  2. Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes.
    Authors: Ohnuma K, Hatano R, Aune T, Otsuka H, Iwata S, Dang N, Yamada T, Morimoto C
    J Immunol, 2015-03-18;194(8):3697-712.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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