Human EBI3 Antibody

Catalog #: MAB6456
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6456
MAB6456-SP

Detection of IL‑27/IL-35 EBI3 Subunit in KG-1 Human Cell Line by Flow Cytometry.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EBI3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-27/IL-35 EBI3 Subunit in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 EBI3 or rmIL-27 (EBI3 + p28 heterodimer) is observed.

Source

Monoclonal Mouse IgG₁ Clone # 607201

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-27/IL-35 EBI3 Subunit
Arg21-Lys229 (predicted)
Accession # Q14213

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL-27/IL-35 EBI3 Subunit antibody in KG-1 Human Cell Line antibody by Flow Cytometry.

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Detection of IL‑27/IL-35 EBI3 Subunit in KG-1 Human Cell Line by Flow Cytometry. KG-1 human acute myelogenous leukemia cell line treated with 40 ng/mL Recombinant Human IL-18/IL-1F4 (Catalog # B001-5) for 24 hrs was stained with Human IL-27/IL-35 EBI3 Subunit Monoclonal Antibody (Catalog # MAB6456, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBI3

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.

Long Name

Epstein-Barr virus induced gene 3

Entrez Gene IDs

10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey)

Alternate Names

cytokine receptor; EBI3; EBI-3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 beta; IL-27 subunit beta; IL27B; IL-27B; IL35 EBI3; IL-35 EBI3; interleukin-27 subunit beta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human EBI3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Human neutrophils activated by TLR8 agonists, with or without IFNgamma, synthesize and release EBI3, but not IL-12, IL-27, IL-35, or IL-39
Authors: MA Cassatella, E Gardiman, F Arruda-Sil, F Bianchetto, S Gasperini, M Bugatti, W Vermi, F Larousseri, O Devergne, N Tamassia
J. Leukoc. Biol., 2020-06-01;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Multifaceted Analysis of IL-23A- and/or EBI3-Including Cytokines Produced by Psoriatic Keratinocytes
Authors: Kota Tachibana, Nina Tang, Hitoshi Urakami, Ai Kajita, Mina Kobashi, Hayato Nomura et al.
International Journal of Molecular Sciences