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Human IL-27 R alpha /WSX-1/TCCR PE-conjugated Antibody

Catalog #: FAB14791P
17 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB14791P-100
FAB14791P-025
Detection of IL‑27 R alpha /WSX‑1/TCCR in Human PBMC lymphocytes by Flow Cytometry.
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Citations (17)
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Human IL-27 R alpha /WSX-1/TCCR PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-27 R alpha /WSX‑1/TCCR in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human gp130 or  recombinant mouse IL-27 Ra is observed.
Source
Monoclonal Mouse IgG2B Clone # 191106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-27 R alpha /WSX‑1/TCCR
Gly34-Lys516
Accession # Q6UWB1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
Human peripheral blood mononuclear cell (PBMC) lymphocytes; U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-27 Ra/WSX-1/TCCR antibody in Human PBMC lymphocytes antibody by Flow Cytometry. View Larger

Detection of IL‑27 R alpha /WSX‑1/TCCR in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and either (A) Mouse Anti-Human IL-27 Ra/WSX-1/TCCR PE-conjugated Monoclonal Antibody (Catalog # FAB14791P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (IC0041P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of IL‑27 R alpha /WSX‑1/TCCR in U937 cells by Flow Cytometry U937 cells were stained with Mouse Anti-Human IL‑27 R alpha /WSX‑1/TCCR PE‑conjugated Monoclonal Antibody (Catalog # FAB14791P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-27 R alpha/WSX-1/TCCR

IL‑27 R alpha (also known as WSX‑1 and TCCR) is a 96‑100 kDa member of the type I, group 2 cytokine receptor family (1 - 6). Mature IL‑27 R alpha  is a type I transmembrane glycoprotein that contains a 484 amino acid (aa) extracellular region, a 21 aa transmembrane segment and a 99 aa cytoplasmic domain. Consistent with type I cytokine receptors, the extracellular region contains four positionally conserved cysteine residues, a WSxWS motif (for receptor folding and ligand binding), and three fibronectin type III repeats. The intracellular domain contains a "box‑1" motif that may be involved with Janus kinases (3). One potential alternate splice form has been hypothesized that involves a 58 aa addition to the cytoplasmic domain and, based on mouse, a soluble 33 kDa splice form that shows a 20 aa substitution for aa 257‑636 may also occur in human (3, 7). The human IL‑27 R alpha extracellular region shares 63% amino acid identity with the mouse IL‑27 R alpha extracellular domain (2, 3). IL‑27 R alpha is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells (1, 2, 4, 8). Typical of other class I cytokine receptor chains, the ligand binding IL‑27 R alpha molecule is known to heterodimerize with a signal‑transducing subunit (gp130) to form a functional IL‑27 receptor (9, 10). In addition, IL‑27 R alpha is reported to complex with CNTFR alpha and gp130 form a humanin receptor on neurons (7, 11), and to complex with gp130 and IL‑6 R to form a receptor for a p28:CLF heterodimeric cytokine on lymphocytes (12). Studies using IL‑27 R alpha /WSX‑1‑/‑ mice reveal that IL‑27 has the ability to suppress T cell activity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity (4, 13, 14). In particular, IL‑27 is known to act on naïve T cells, blocking their differentiation into a Th17 phenotype. Notably, cells committed to a Th17 phenotype, although they express a functional IL‑27 receptor, are unresponsive to the effects of IL‑27 (15). Activated T cells that are CD4+ and CD8+, and which express the IL‑27 receptor, can be induced by IL‑27 to form a double‑positive CD25+ FoxP3‑ IFN‑ gamma plus IL‑10 secreting phenotype that both promotes and suppresses the inflammatory response (16).

References
  1. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  2. Chen, Q. et al. (2000) Nature 407:916.
  3. Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82.
  4. Artis, D. et al. (2004) J. Immunol. 173:5626.
  5. Yoshida, H. & Y. Miyazaki (2008) Int. J. Biochem. Cell Biol. 40:2379.
  6. Yoshida, H. & M. Yoshiyuki (2008) Immunol. Rev. 226:234.
  7. Hashimoto, Y. et al. (2009) Biochem. Biophys. Res. Commun. 389:95.
  8. Holscher, C. et al. (2005) J. Immunol. 174:3534.
  9. Pflanz, S. et al. (2004) J. Immunol. 172:2225.
  10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  11. Hashimoto, Y. et al. (2009) Mol. Biol. Cell 20:2864.
  12. Crabe, S. et al. (2009) J. Immunol. 183:7692.
  13. Villarino, A. et al. (2003) J. Immunol. 170:645.
  14. Hamano., S. et al. (2003) Immunity 19:657.
  15. El-behi, M. et al. (2009) J. Immunol. 183:4957.
  16. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Long Name
Interleukin-27 Receptor Subunit alpha
Entrez Gene IDs
9466 (Human); 50931 (Mouse)
Alternate Names
class I cytokine receptor; CRL1IL-27R; Cytokine receptor-like 1; IL-27 R alpha; IL27R alpha; IL27R; IL27RA; IL-27Ra; IL-27R-alpha; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; TCCR; TCCRIL-27 receptor subunit alpha; T-cell cytokine receptor type 1; Type I T-cell cytokine receptor; WSX-1; WSX1IL-27R subunit alpha; zcytor1

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Citations for Human IL-27 R alpha /WSX-1/TCCR PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Interleukin-35 promotes Breg expansion and interleukin-10 production in CD19+ B cells in patients with ankylosing spondylitis
    Authors: Yu Zhang, Sixi Wei, Qingqing Wu, Xue Shen, Wanrong Dai, Zhiqi Zhang et al.
    Clinical Rheumatology
  2. Role of IL-27 in Epstein-Barr virus infection revealed by IL-27RA deficiency
    Authors: Martin, E;Winter, S;Garcin, C;Tanita, K;Hoshino, A;Lenoir, C;Fournier, B;Migaud, M;Boutboul, D;Simonin, M;Fernandes, A;Bastard, P;Le Voyer, T;Roupie, AL;Ben Ahmed, Y;Leruez-Ville, M;Burgard, M;Rao, G;Ma, CS;Masson, C;Soudais, C;Picard, C;Bustamante, J;Tangye, SG;Cheikh, N;Seppänen, M;Puel, A;Daly, M;Casanova, JL;Neven, B;Fischer, A;Latour, S;
    Nature
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Glycosaminoglycans bind human IL-27 and regulate its activity
    Authors: MC Cavé, S Maillard, K Hildenbran, C Mamelonet, MJ Feige, O Devergne
    Eur. J. Immunol., 2020-07-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Increased KLRG1 and PD-1 expression on CD8 T lymphocytes in TB-IRIS
    Authors: O Goovaerts, M Massinga-L, P Ondoa, A Ceulemans, W Worodria, H Mayanja-Ki, R Colebunder, L Kestens
    PLoS ONE, 2019-04-25;14(4):e0215991.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies
    Authors: C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan
    J. Leukoc. Biol., 2018-07-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations
    Authors: F Xu, J Yi, Z Wang, Y Hu, C Han, Q Xue, X Zhang, X Luan
    Immunol. Res., 2017-08-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. IL-27 Directly Enhances Germinal Center B Cell Activity and Potentiates Lupus in Sanroque Mice
    J Immunol, 2016-09-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. A soluble form of IL-27Ralpha is a natural IL-27 antagonist.
    Authors: Dietrich C, Candon S, Ruemmele F, Devergne O
    J Immunol, 2014-04-25;192(11):5382-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-gamma release by autologous NK cells.
    Authors: Morandi F, Airoldi I, Pistoia V
    J Immunol Res, 2014-03-10;2014(0):938561.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014-02-19;192(6):2634-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
    Authors: Tzeng, Tsai-Ten, Tsay, Huey-Jen, Chang, Luping, Hsu, Chia-Lin, Lai, Tzu-Hsua, Huang, Fong-Lee, Shiao, Young-Ji
    J Biomed Sci, 2013-12-06;20(0):90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer.
    Authors: Kachroo P, Lee M, Zhang L, Baratelli F, Lee G, Srivastava M, Wang G, Walser T, Krysan K, Sharma S, Dubinett S, Lee J
    J Exp Clin Cancer Res, 2013-11-25;32(0):97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway.
    Authors: Chen, Qian, Swaminathan, Sanjay, Yang, De, Dai, Lue, Sui, Hongyan, Yang, Jun, Hornung, Ronald L, Wang, Yanmei, Huang, Da Wei, Hu, Xiaojun, Lempicki, Richard, Imamichi, Tomozumi
    PLoS ONE, 2013-03-20;8(3):e59194.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. IL-27-Induced Gene Expression Is Downregulated in HIV-Infected Subjects.
    Authors: Guzzo C, Hopman W, Che Mat N, Wobeser W, Gee K
    PLoS ONE, 2012-09-25;7(9):e45706.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
    Authors: Cocco C, Di Carlo E, Zupo S
    Leukemia, 2011-12-23;26(6):1365-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. IL-21–Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B
    Authors: Arshi Khanam, Natarajan Ayithan, Lydia Tang, Bhawna Poonia, Shyam Kottilil
    Frontiers in Immunology
  17. IL-27 induces IFN/STAT1-dependent genes and enhances function of TIGIT+ HIVGag-specific T cells
    Authors: Jie Cheng, Timothy G. Myers, Callie Levinger, Princy Kumar, Jai Kumar, Bruktawit A. Goshu et al.
    iScience

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