Human IL-32 Antibody

Catalog #: MAB4690 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB4690-100
MAB4690-SP

Detection of Human IL‑32 by Western Blot.

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All IL-32 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-32 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-32 gamma and other IL-32 isoforms in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) IL-32 alpha and 100% cross-reactivity with rhIL-32 beta is detected.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2526B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human IL‑32 gamma protein
Met1-Lys188
Accession # P24001

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑32 by Western Blot. Western blot shows lysates of HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human IL-32 Monoclonal Antibody (Catalog # MAB4690) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-32 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-32

Interleukin 32 (IL-32) is an N-glycosylated cytokine that is upregulated by inflammatory stimulation in monocytes, NK cells, epithelial cells, and pancreatic myofibroblasts (1-5). It cooperates with these stimuli to promote the expression of other proinflammatory molecules such as TNF-alpha, IL-6, IL-1 beta, IL-1 alpha, and CXCL8/IL‑8 (5-7). The longest of several IL-32 splicing variants is the 20-25 kDa gamma isoform which is also known as natural killer cell transcript 4 (NK4) (8, 9). The alpha isoform (IL-32 alpha ) lacks a portion of the putative signal peptide as well as 57 aa from the C-terminal region. IL-32 alpha is less potent than IL-32 beta, gamma, or δ at inducing the expression of proinflammatory molecules in peripheral blood mononuclear cells (PBMC) (8, 10). Neutrophil-derived Proteinase 3 (PR3) cleaves IL-32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL-32 isoforms (11). The N-terminal fragment of PR3-cleaved IL-32 alpha shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32 alpha (11, 12). IL-32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (5, 13-15). IL-32 inhibits HIV-1 replication in vitro, and it is elevated in the serum of HIV-1 patients (16, 17).

References

Netea, M.G. et al. (2006) PloS Med. 3:e277.
Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3883.
Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
Nishida, A. et al. (2008) Am. J. Physiol. Gastrointest. Liver Physiol. 294:G831.
Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. USA 102:16309.
Hong, J. et al. (2010) Cytokine 49:171.
Kim, S-H. et al. (2005) Immunity 22:131.
Dahl, C.A. et al. (1992) J. Immunol. 148:597.
Choi, J-D. et al. (2009) Immunology 126:535.
Novick, D. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3316.
Kim, S. et al. (2008) BMB Rep. 41:814.
Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3298.
Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
Nold, M.F. et al. (2008) J. Immunol. 181:557.

Long Name

Interleukin 32

Entrez Gene IDs

9235 (Human)

Alternate Names

IL32; IL-32; IL-32alpha; IL-32beta; interleukin 32; interleukin-32 theta; interleukin-32; natural killer cell transcript 4; Natural killer cells protein 4; NK4; NK4IL-32delta; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; TAIFIL-32gamma; Tumor necrosis factor alpha-inducing factor