Human IL-32 Antibody

Catalog #: MAB30402 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB30402-100
MAB30402-SP

Detection of IL‑32 in Human PBMCs by Flow Cytometry.

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All IL-32 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-32 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-32 alpha and other IL-32 isoforms in direct ELISAs. In direct ELISAs, 30% cross-reactivity with recombinant human (rh) IL-32 beta and 38% cross-reactivity with rhIL-32 gamma is detected.

Source

Monoclonal Rat IgG_2A Clone # 373821

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL-32 alpha
Met1-Asn131
Accession # P24001

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL-32 antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of IL‑32 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were stained with Rat Anti-Human IL-32 Monoclonal Antibody (Catalog # MAB30402, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-32

Interleukin 32 (IL-32) is an N-glycosylated cytokine that is upregulated by inflammatory stimulation in monocytes, NK cells, epithelial cells, and pancreatic myofibroblasts (1-5). It cooperates with these stimuli to promote the expression of other proinflammatory molecules such as TNF-alpha, IL-6, IL-1 beta, IL-1 alpha, and CXCL8/IL‑8 (5-7). The longest of several IL-32 splicing variants is the 20-25 kDa gamma isoform which is also known as natural killer cell transcript 4 (NK4) (8, 9). The alpha isoform (IL-32 alpha ) lacks a portion of the putative signal peptide as well as 57 aa from the C-terminal region. IL-32 alpha is less potent than IL-32 beta, gamma, or δ at inducing the expression of proinflammatory molecules in peripheral blood mononuclear cells (PBMC) (8, 10). Neutrophil-derived Proteinase 3 (PR3) cleaves IL-32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL-32 isoforms (11). The N-terminal fragment of PR3-cleaved IL-32 alpha shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32 alpha (11, 12). IL-32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (5, 13-15). IL-32 inhibits HIV-1 replication in vitro, and it is elevated in the serum of HIV-1 patients (16, 17).

References

Netea, M.G. et al. (2006) PloS Med. 3:e277.
Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3883.
Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
Nishida, A. et al. (2008) Am. J. Physiol. Gastrointest. Liver Physiol. 294:G831.
Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. USA 102:16309.
Hong, J. et al. (2010) Cytokine 49:171.
Kim, S-H. et al. (2005) Immunity 22:131.
Dahl, C.A. et al. (1992) J. Immunol. 148:597.
Choi, J-D. et al. (2009) Immunology 126:535.
Novick, D. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3316.
Kim, S. et al. (2008) BMB Rep. 41:814.
Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3298.
Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
Nold, M.F. et al. (2008) J. Immunol. 181:557.

Long Name

Interleukin 32

Entrez Gene IDs

9235 (Human)

Alternate Names

IL32; IL-32; IL-32alpha; IL-32beta; interleukin 32; interleukin-32 theta; interleukin-32; natural killer cell transcript 4; Natural killer cells protein 4; NK4; NK4IL-32delta; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; TAIFIL-32gamma; Tumor necrosis factor alpha-inducing factor