Human IL-32 alpha Antibody Summary
Phe3-Lys131
Accession # NP_001012651
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL‑32 alpha by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) treated (+) with 200 ng/mL Ionomycin and 10 ng/mL PMA for 72 hours. PVDF Membrane was probed with 2 µg/mL of Human IL-32a Monoclonal Antibody (Catalog # MAB30401) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-32a at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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IL‑32 alpha in Human PBMCs. IL-32a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-32a Monoclonal Antibody (Catalog # MAB30401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-32 alpha
IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.
Product Datasheets
Citations for Human IL-32 alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1
Authors: RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi
Sci Adv, 2018-02-21;4(2):e1701729.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Increased Interleukin-32 Levels in Obesity Promote Adipose Tissue Inflammation and Extracellular Matrix Remodeling: Effect of Weight Loss
Authors: Victoria Catalán
Diabetes, 2016-09-14;65(12):3636-3648.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein.
Authors: Hasegawa H, Thomas HJ, Schooley K, Born TL
Cytokine, 2011-01-01;53(1):74-83.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Did not work with human lung sections without retreival or with citrate retreival - IgG was used as control.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
