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Human IL-33 Antibody

Catalog #: AF3625
53 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3625
AF3625-SP
Cell Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody.
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Product Details
Citations (53)
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Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-33 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-33 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) IL‑33 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-33
Ser112-Thr270
Accession # O95760
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑33 (Catalog # 3625-IL)
Immunohistochemistry
5-15 µg/mL
See below
Dual RNAscope ISH-IHC
3-25 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells treated with PMA and calcium ionomycin

Human IL-33 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human IL‑33 Biotinylated Antibody (Catalog # BAF3625)

Standard: Recombinant Human IL-33 Protein (Catalog # 3625-IL)

Neutralization
Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑33 and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody. View Larger

Cell Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody. In the presence of sub-optimal amounts of Hamster Anti-Mouse CD3e Monoclonal Antibody (Catalog # MAB484), Recom-binant Human IL-33 (Catalog # 3625-IL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human IL-33 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625). The ND50 is typically 0.75-3.0 µg/mL.

Immunohistochemistry IL-33 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

IL‑33 in Human Tonsil. IL-33 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Dual RNAscope ISH-IHC Compatible IL-33 antibody in Human Tonsil Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

IL-33 in Human Tonsil Using Dual RNAscope®ISH and IHC. IL-33 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 400111) and stained using ACD RNAscope®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625) at 3 ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is up‑regulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is up‑regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL-33 share 52‑58% aa sequence identity with mouse and rat IL-33. Human IL-33 shares less than 20% aa sequence identity with other IL-1 family proteins.

References
  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Long Name
Interleukin 33
Entrez Gene IDs
90865 (Human); 77125 (Mouse)
Alternate Names
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

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Citations for Human IL-33 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

53 Citations: Showing 1 - 10
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  1. The IL-33:ST2 axis is unlikely to play a central fibrogenic role in idiopathic pulmonary fibrosis
    Authors: KE Stephenson, J Porte, A Kelly, WA Wallace, CE Huntington, CL Overed-Say, ES Cohen, RG Jenkins, AE John
    Respiratory Research, 2023-03-23;24(1):89.
  2. Oncogenic KRAS-dependent stromal interleukin-33 directs the pancreatic microenvironment to promote tumor growth
    Authors: Donahue, KL;Watkoske, HR;Kadiyala, P;Du, W;Brown, K;Scales, MK;Elhossiny, AM;Espinoza, CE;Lasse Opsahl, EL;Griffith, BD;Wen, Y;Sun, L;Velez-Delgado, A;Renollet, NM;Morales, J;Nedzesky, NM;Baliira, RK;Menjivar, RE;Medina-Cabrera, PI;Rao, A;Allen, B;Shi, J;Frankel, TL;Carpenter, ES;Bednar, F;Zhang, Y;Pasca di Magliano, M;
    Cancer discovery
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: Immunohistochemistry, Western Blot
  3. Activator protein transcription factors coordinate human IL-33 expression from noncanonical promoters in chronic airway disease
    Authors: Raphael, HE;Hassan, GF;Osorio, OA;Cohen, LS;Payne, MD;Katz-Kiriakos, E;Tata, I;Hicks, J;Byers, DE;Zhang, B;Alexander-Brett, J;
    JCI insight
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosis
    Authors: Jeon, YJ;Gil, CH;Won, J;Jo, A;Kim, HJ;
    BMC microbiology
    Species: Mouse
    Sample Types: Nasal Lavage Fluid
    Applications: Western Blot
  5. Imatinib inhibits pericyte-fibroblast transition and inflammation and promotes axon regeneration by blocking the PDGF-BB/PDGFR? pathway in spinal cord injury
    Authors: Yao F, Luo Y, Liu YC et al.
    Inflammation and Regeneration
  6. Organoids capture tissue-specific innate lymphoid cell development in mice and humans
    Authors: GM Jowett, E Read, LB Roberts, D Coman, M Vilà Gonzá, T Zabinski, U Niazi, R Reis, TJ Trieu, D Danovi, E Gentleman, L Vallier, MA Curtis, GM Lord, JF Neves
    Cell Reports, 2022-08-30;40(9):111281.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Human Melanoma-Associated Mast Cells Display a Distinct Transcriptional Signature Characterized by an Upregulation of the Complement Component 3 That Correlates With Poor Prognosis
    Authors: Rajia Bahri, Orsolya Kiss, Ian Prise, Karen M. Garcia-Rodriguez, Haris Atmoko, Julia M. Martínez-Gómez et al.
    Frontiers in Immunology
  8. Fungal mycobiome drives IL-33 secretion and type 2 immunity in pancreatic cancer
    Authors: Aftab Alam, Eric Levanduski, Parker Denz, Helena Solleiro Villavicencio, Maulasri Bhatta, Lamees Alhorebi et al.
    Cancer Cell
  9. Nuclear IL-33 regulates cytokinesis and cell motility in normal human epidermal keratinocytes
    Authors: H Tsuda, SI Tominaga, M Ohtsuki, M Komine
    Journal of dermatological science, 2022-01-25;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Interleukin-33 activates group 2 innate lymphoid cell expansion and modulates endometriosis
    Authors: JE Miller, H Lingegowda, LK Symons, O Bougie, SL Young, BA Lessey, M Koti, C Tayade
    JCI Insight, 2021-12-08;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IF
  11. IL-33 Depletion in COVID-19 Lungs
    Authors: Rohit Gaurav, Daniel R. Anderson, Stanley J. Radio, Kristina L. Bailey, Bryant R. England, Ted R. Mikuls et al.
    Chest
  12. Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema
    Authors: BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan
    Biology, 2021-09-18;10(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung disease
    Authors: TR Mikuls, R Gaurav, GM Thiele, BR England, MG Wolfe, BP Shaw, KL Bailey, TA Wyatt, AJ Nelson, MJ Duryee, CD Hunter, D Wang, DJ Romberger, DP Ascherman, JA Poole
    International immunopharmacology, 2021-08-27;100(0):108069.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. PPAR? drives IL-33-dependent ILC2 pro-tumoral functions
    Authors: G Ercolano, A Gomez-Cade, N Dumauthioz, G Vanoni, M Kreutzfeld, T Wyss, L Michalik, R Loyon, A Ianaro, PC Ho, C Borg, M Kopf, D Merkler, P Krebs, P Romero, S Trabanelli, C Jandus
    Nature Communications, 2021-05-05;12(1):2538.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Fibroblast-derived IL-33 facilitates breast cancer metastasis by modifying the immune microenvironment and driving type-2 immunity
    Authors: Ophir Shani, Tatiana Vorobyov, Lea Monteran, Dor Lavie, Noam Cohen, Yael Raz et al.
    Cancer Research
  16. IL-33 Alarmin and Its Active Proinflammatory Fragments Are Released in Small Intestine in Celiac Disease
    Authors: Federico Perez, Carolina N. Ruera, Emanuel Miculan, Paula Carasi, Karen Dubois-Camacho, Laura Garbi et al.
    Frontiers in Immunology
  17. Symbiotic microbiome Staphylococcus aureus from human nasal mucus modulates IL-33-mediated type 2 immune responses in allergic nasal mucosa
    Authors: YJ Jeon, CH Gil, J Won, A Jo, HJ Kim
    BMC Microbiol, 2020-10-07;20(1):301.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. High level of interleukin-33 in cancer cells and cancer-associated fibroblasts correlates with good prognosis and suppressed migration in cholangiocarcinoma
    Authors: S Yangngam, S Thongchot, A Pongpaibul, K Vaeteewoot, S Pinlaor, P Thuwajit, S Okada, MA Hermoso, C Thuwajit
    J Cancer, 2020-09-23;11(22):6571-6581.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  19. Interleukin-33 Signaling Controls the Development of Iron-Recycling Macrophages
    Authors: Yuning Lu, Gemma Basatemur, Ian C. Scott, Davide Chiarugi, Marc Clement, James Harrison et al.
    Immunity
  20. Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular Lesions
    Authors: Suresh Govatati, Prahalathan Pichavaram, Jagadeesh Janjanam, Liang Guo, Renu Virmani, Gadiparthi N. Rao
    Arteriosclerosis, Thrombosis, and Vascular Biology
  21. ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity
    Authors: JA Moral, J Leung, LA Rojas, J Ruan, J Zhao, Z Sethna, A Ramnarain, B Gasmi, M Gururajan, D Redmond, G Askan, U Bhanot, E Elyada, Y Park, DA Tuveson, M Gönen, SD Leach, JD Wolchok, RP DeMatteo, T Merghoub, VP Balachandr
    Nature, 2020-02-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. IL-33/ST2 axis promotes glioblastoma cell invasion by accumulating tenascin-C
    Authors: JF Zhang, T Tao, K Wang, GX Zhang, Y Yan, HR Lin, Y Li, MW Guan, JJ Yu, XD Wang
    Sci Rep, 2019-12-30;9(1):20276.
    Species: Human
    Sample Types: Tissue
    Applications: IHC
  23. Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis
    Authors: D Izadi, TB Layton, L Williams, F McCann, M Cabrita, AI Espirito S, W Xie, M Fritzsche, H Colin-York, M Feldmann, KS Midwood, J Nanchahal
    Sci Adv, 2019-12-04;5(12):eaay0370.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis
    Authors: Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su
    Oncogene, 2019-10-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  25. Interleukin 33/ST2 Axis Components Are Associated to Desmoplasia, a Metastasis-Related Factor in Colorectal Cancer
    Authors: Glauben Landskron, Marjorie De la Fuente López, Karen Dubois-Camacho, David Díaz-Jiménez, Octavio Orellana-Serradell, Diego Romero et al.
    Frontiers in Immunology
  26. IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection
    Authors: AL Frisbee, MM Saleh, MK Young, JL Leslie, ME Simpson, MM Abhyankar, CA Cowardin, JZ Ma, P Pramoonjag, SD Turner, AP Liou, EL Buonomo, WA Petri
    Nat Commun, 2019-06-20;10(1):2712.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  27. NFATc1-E2F1-LMCD1–Mediated IL-33 Expression by Thrombin Is Required for Injury-Induced Neointima Formation
    Authors: Suresh Govatati, Prahalathan Pichavaram, Jagadeesh Janjanam, Baolin Zhang, Nikhlesh K. Singh, Arul M. Mani et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  28. Hedgehog Signaling Modulates Interleukin‐33‐Dependent Extrahepatic Bile Duct Cell Proliferation in Mice
    Authors: Nataliya Razumilava, Junya Shiota, Nureen H. Mohamad Zaki, Ramon Ocadiz‐Ruiz, Christine M. Cieslak, Kais Zakharia et al.
    Hepatology Communications
  29. Neoplastic Transformation of the Peribiliary Stem Cell Niche in Cholangiocarcinoma Arisen in Primary Sclerosing Cholangitis
    Authors: Guido Carpino, Vincenzo Cardinale, Trine Folseraas, Diletta Overi, Krzysztof Grzyb, Daniele Costantini et al.
    Hepatology
  30. Cellular and clinicopathological features of the IL-33/ST2 axis in human esophageal squamous cell carcinomas
    Authors: G Cui, J Ren, G Xu, Z Li, W Zheng, A Yuan
    Cancer Cell Int., 2018-12-11;18(0):203.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  31. Dysregulated invertebrate tropomyosin-dectin-1 interaction confers susceptibility to allergic diseases
    Authors: N Gour, S Lajoie, U Smole, M White, D Hu, P Goddard, S Huntsman, C Eng, A Mak, S Oh, JH Kim, A Sharma, S Plante, IH Salem, Y Resch, X Xiao, N Yao, A Singh, S Vrtala, J Chakir, EG Burchard, AP Lane, M Wills-Karp
    Sci Immunol, 2018-02-23;3(20):.
    Species: Human, Mouse
    Sample Types: BALF, Cell Culture Supernates
    Applications: ELISA Capture
  32. HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33
    Authors: Megan Osbourn, Dinesh C. Soares, Francesco Vacca, E. Suzanne Cohen, Ian C. Scott, William F. Gregory et al.
    Immunity
  33. Epithelial-derived IL-33 promotes intestinal tumorigenesis in Apc Min/+ mice
    Authors: Zhengxiang He, Lili Chen, Fabricio O. Souto, Claudia Canasto-Chibuque, Gerold Bongers, Madhura Deshpande et al.
    Scientific Reports
  34. IL‐10‐producing, ST2‐expressing Foxp3 + T cells in multiple sclerosis brain lesions
    Authors: Stephanie Elizabeth Johanna Zandee, Richard Anthony O'Connor, Iris Mair, Melanie Dawn Leech, Anna Williams, Stephen Mark Anderton
    Immunology & Cell Biology
  35. The ”Alarmins” HMBG1 and IL-33 Downregulate Structural Skin Barrier Proteins and Impair Epidermal Growth
    Authors: Uffe Nygaard, Ellen H van den Bogaard, Hanna Niehues, Malene Hvid, Mette Deleuran, Claus Johansen et al.
    Acta Dermato Venereologica
  36. Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis
    PLoS ONE, 2016-12-19;11(12):e0168080.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Loss of interleukin 33 expression in colonic crypts - a potential marker for disease remission in ulcerative colitis
    Authors: Mona Dixon Gundersen, Rasmus Goll, Johanna Hol, Trine Olsen, Renathe Rismo, Sveinung W. Sørbye et al.
    Scientific Reports
  38. TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells
    J Immunol, 2016-08-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. Interleukin-33 in human gliomas: Expression and prognostic significance
    Authors: DOROTHEE GRAMATZKI, KARL FREI, GIERI CATHOMAS, HOLGER MOCH, MICHAEL WELLER, KIRSTEN DIANA MERTZ
    Oncology Letters
  40. Role of IL-33 expression in oncogenesis and development of human hepatocellular carcinoma
    Authors: YAN YANG, JUN-BIN WANG, YU-MEI LI, Y U ZHAO, RUI WANG, QIONG WU et al.
    Oncology Letters
  41. The IL-33/ST2 pathway contributes to intestinal tumorigenesis in humans and mice
    Authors: Kirsten D. Mertz, Lukas F. Mager, Marie-Hélène Wasmer, Thore Thiesler, Viktor H. Koelzer, Giulia Ruzzante et al.
    OncoImmunology
  42. IL-33 Signaling Protects from Murine Oxazolone Colitis by Supporting Intestinal Epithelial Function
    Authors: Amanda Waddell, Jefferson E. Vallance, Preston D. Moore, Amy T. Hummel, David Wu, Shiva K. Shanmukhappa et al.
    Inflammatory Bowel Diseases
  43. Oxidation of the alarmin IL-33 regulates ST2-dependent inflammation
    Authors: E. Suzanne Cohen, Ian C. Scott, Jayesh B. Majithiya, Laura Rapley, Benjamin P. Kemp, Elizabeth England et al.
    Nature Communications
  44. IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms.
    Authors: Mager L, Riether C, Schurch C, Banz Y, Wasmer M, Stuber R, Theocharides A, Li X, Xia Y, Saito H, Nakae S, Baerlocher G, Manz M, McCoy K, Macpherson A, Ochsenbein A, Beutler B, Krebs P
    J Clin Invest, 2015-05-26;125(7):2579-91.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  45. Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences.
    Authors: Sundnes O, Pietka W, Loos T, Sponheim J, Rankin A, Pflanz S, Bertelsen V, Sitek J, Hol J, Haraldsen G, Khnykin D
    J Invest Dermatol, 2015-03-04;135(7):1771-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  46. Neutralisation of the interleukin-33/ST2 pathway ameliorates experimental colitis through enhancement of mucosal healing in mice
    Authors: Mamdouh A K Sedhom, Mélanie Pichery, Jenna R Murdoch, Benoit Foligné, Nathalie Ortega, Sylvain Normand et al.
    Gut
  47. The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis.
    Authors: Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T
    Clin. Exp. Allergy, 2012-02-01;42(2):218-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  48. Distinct serum and synovial fluid interleukin (IL)-33 levels in rheumatoid arthritis, psoriatic arthritis and osteoarthritis
    Authors: Dominique Talabot-Ayer, Thomas McKee, Patrizia Gindre, Sylvette Bas, Dominique L. Baeten, Cem Gabay et al.
    Joint Bone Spine
  49. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon SJ, Moon S, Joo YB, Joo Y, Jeon CH, Jeon C, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 2011-09-01;26(9):1132-9.
    Species: Human
    Sample Types: Synovial Fluid, Whole Tissue
    Applications: ELISA Development, IHC-P
  50. The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation.
    Authors: Byrne SN, Beaugie C, O'Sullivan C, Leighton S, Halliday GM
    Am. J. Pathol., 2011-05-13;179(1):211-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  51. Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts.
    Authors: Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y
    Gut, 2009-12-08;59(4):531-41.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  52. Inhibition of interleukin-33 signaling attenuates the severity of experimental arthritis.
    Authors: Palmer G, Talabot-Ayer D, Lamacchia C, Toy D, Seemayer CA, Viatte S, Finckh A, Smith DE, Gabay C
    Arthritis Rheum., 2009-03-01;60(3):738-49.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  53. Eosinophils regulate intra-adipose axonal plasticity
    Authors: Meng X, Qian X, Ding X et al.
    Proceedings of the National Academy of Sciences of the United States of America

FAQs

  1. Does the Human IL-33 Antibody, Catalog # AF3625, detect full length IL-33 or the cleaved forms?

    • The immunogen sequence that this antibody recognizes is Ser112-Thr270 from Accession # O95760. This seqeunce corresponds to the cleaved form and is included in the full length sequence. Therefore, we would expect that this antibody detects both the full length and cleaved forms.

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Human IL-33 Antibody
By Anonymous on 07/25/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Aorta tissue Species: Human
Immunocytochemistry/Immunofluorescence Human IL-33 Antibody AF3625
Human IL-33 Antibody
By Anonymous on 06/09/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody in an in-house ELISA along with mAb (3625) and protein (3625-IL-010) to quantify IL-33 in human serum and plasma. This combination could not detect IL-33 in our samples but generated a good standard curve.

ELISA Human IL-33 Antibody AF3625
Human IL-33 Antibody
By Anonymous on 02/26/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: BRONCHUS Species: Human

Staining was done on human airways after citrate antigen retrieval. OCT sections were used

Immunocytochemistry/Immunofluorescence Human IL-33 Antibody AF3625
Human IL-33 Antibody
By Stephanie Zandee on 03/27/2018
Application: IHC Sample Tested: Adult brain Species: Human

Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287
Used at 10ug/ml.
Briefly, frozen brain sections were fixed in 4% PFA
(Fisher Scientific), followed by antigen retrieval using heating in acid
citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector).
Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor
Blocking Solution was added (Human TruStain FcX Biolegend,
London, UK). Primary antibodies were added overnight at 4 °C.
IL-33 was detected with donkey anti-goat-biotin (ab6578,
Abcam), followed by streptavidin-HRP and visualised with DAB. Section was counterstained with Hematoxylin.

Immunohistochemistry Human IL-33 Antibody AF3625
Human IL-33 Antibody
By Anonymous on 11/09/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

AF3625 was used as both the capture and the detection antibody for the sandwich ELISA for IL-33. The immunoassay standard was 3625-IL. Assay had sensitivity of ~1pg/ml. Many healthy human samples were not detectable.

ELISA Human IL-33 Antibody AF3625
Human IL-33 Antibody
By Anonymous on 10/18/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult lung Species: Human
Immunocytochemistry/Immunofluorescence Human IL-33 Antibody AF3625