Human IL-34 PE-conjugated Antibody Summary
Asn21-Pro242
Accession # Q6ZMJ4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑34 in NS0 Mouse Cell Line Transfected with Human IL-34 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human IL-34 was stained with Mouse Anti-Human IL-34 PE-conjugated Monoclonal Antibody (Catalog # IC5265P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: IL-34
Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1,2). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1,3).
Product Datasheets
Citations for Human IL-34 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Cross-Reactive Donor-Specific CD8+ Tregs Efficiently Prevent Transplant Rejection
Authors: E Picarda, S Bézie, L Usero, J Ossart, M Besnard, H Halim, K Echasserie, C Usal, J Rossjohn, K Bernardeau, S Gras, C Guillonnea
Cell Rep, 2019-12-24;29(13):4245-4255.e6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-34 is a Treg-specific cytokine and mediates transplant tolerance.
Authors: Bezie S, Picarda E, Ossart J, Tesson L, Usal C, Renaudin K, Anegon I, Guillonneau C
J Clin Invest, 2015-09-21;125(10):3952-64.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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