Human IL-36 alpha /IL-1F6 Antibody Summary
Met1-Phe158
Accession # Q9UHA7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL‑36 alpha /IL‑1F6 by Western Blot. Western blot shows lysates of human tonsil tissue, HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-36a/IL-1F6. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-36a/IL-1F6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1078) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-36a/IL-1F6 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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IL‑36 alpha /IL‑1F6 in Human Tonsil. IL-36a/IL-1F6 was detected in perfusion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-36a/IL-1F6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1078) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-36 alpha/IL-1F6
Human interleukin 1 family member #6 [IL-1F6; also Interleukin-36 alpha, IL36A, FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL-1 beta, IL-1 alpha, IL‑1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F6 is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1‑3). It appears to be actively secreted (1). When found in cell lysate, it presents as an 18 kDa monomer (2). Human to mouse, full length IL-1F6 has 54% aa identity. Within the family, IL-1F6 is 30% aa identical to IL-1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL-1 beta, IL-1F5, F7, F8, F9 and F10, respectively. Cells reported to express IL-1F6 include monocytes, B cells and T cells (1, 4). Notably, IL-1F6 is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-1F6 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5).
- Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
- Dunn, E. et al. (2001) Trends Immunol. 22:533.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Product Datasheets
Citations for Human IL-36 alpha /IL-1F6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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A therapeutic strategy of parthenolide in improving imiquimod-induced psoriasis-like skin inflammation targeting IL-36/NETs through skin transdermal therapeutic system
Authors: Zhan, ZY;Zhang, ZH;Sun, RH;Wu, YL;Nan, JX;Lian, LH;
International immunopharmacology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells
Authors: JM Murrieta-C, RN Gutiérrez-, HM El-Shorafa, T Groten, S Rodríguez-, ME Cancino-Di, JC Cancino-Di, RR Favaro, UR Markert, DM Morales-Pr
International Journal of Molecular Sciences, 2020-12-30;22(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Interleukin-36 receptor mediates the crosstalk between plasma cells and synovial fibroblasts
Authors: V Schmitt, M Hahn, V Kästele, O Wagner, M Wiendl, A Derer, A Taddeo, S Hahne, A Radbruch, HM Jäck, W Schuh, D Mielenz, S Gay, G Schett, AJ Hueber, S Frey
Eur. J. Immunol., 2017-09-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
J. Immunol., 2011-01-17;186(4):2613-22.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
IL-1 and IL-36 are dominant cytokines in generalized pustular psoriasis.
Authors: Johnston Andrew, Xing Xianying, Wolterink Liza et al.
The Journal of Allergy and Clinical Immunology
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