Home / IL-36 beta/IL-1F8 / Human IL-36 beta /IL-1F8 Antibody

Human IL-36 beta /IL-1F8 Antibody

Catalog #: MAB1099
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1099
MAB1099-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-36 beta /IL-1F8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑36 beta /IL‑1F8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1ra, rhIL-18, rhIL-36Ra, rhIL-36 alpha, rhIL-1F7 or recombinant mouse IL-36 beta is observed.
Source
Monoclonal Mouse IgG1 Clone # 162601
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑36 beta /IL‑1F8
Met1-Glu157
Accession # NP_775270
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑36 beta /IL‑1F8 (Catalog # 1099-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 beta/IL-1F8

Human interleukin 1 family member #8 [IL-1F8; also named IL-36 beta FIL-1 eta (eta) and IL-1H2] is a member of the IL-1 family of proteins (1‑3, 6). IL-1 family members include IL‑1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -stranded beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Two alternatively spliced transcript variants encode distinct (164 or 157 residues) protein isoforms that differ in their C-terminal 70 amino acid (aa) residues have been reported (3). IL-36 beta isoform 2 is synthesized as a 157 aa protein that contains no signal sequence and no prosegment (1‑2). Unlike IL-36 beta isoform 1 which lacks potential N-linked glycosylation sites, isoform 2 contains one potential N-linked glycosylation site in its unique C-terminus. IL-36 beta is reported to be actively secreted (1). Human IL-36 beta isoform 2 shares 61% aa identity with mouse IL-1 ra, a 183 aa form of IL-36 beta. Within the IL-1 family, IL-36 beta shares 30%, 32%, 37%, 46%, 34%, 45% and 28% aa sequence identity with IL-1 ra, IL-1 beta, IL-36Ra, IL-36 alpha, F7, IL-36 gamma and F10, respectively. Cells reported to express IL-36 beta include resting and activated monocytes and B cells (1, 4). The receptor for IL-36 beta is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-36 beta, along with IL-36 alpha and IL-36 gamma, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

 

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
Long Name
Interleukin 36 beta/Interleukin 1 Family 8
Entrez Gene IDs
27177 (Human); 69677 (Mouse)
Alternate Names
family of interleukin 1-eta; FIL1 eta; FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); IL-1 eta; IL1-ETA; IL1F8 (Canonical product IL-1F8a); IL-1F8 (FIL1-eta); IL1F8; IL-1F8; IL1H2; IL-1H2; IL36 beta; IL-36 beta; IL36B; interleukin 1 family, member 8 (eta); interleukin 1, eta; Interleukin 36, Beta; Interleukin-1 eta; interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 Beta

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human IL-36 beta /IL-1F8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells
    Authors: T Song, D Spillmann
    J. Cell. Mol. Med., 2019-05-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Interleukin-1 family cytokines and their regulatory proteins in normal pregnancy and pre-eclampsia.
    Authors: Southcombe J, Redman C, Sargent I, Granne I
    Clin Exp Immunol, 2015-06-29;181(3):480-90.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
    Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
    J. Immunol., 2011-01-17;186(4):2613-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human IL-36 beta /IL-1F8 Antibody

There are currently no reviews for this product. Be the first to review Human IL-36 beta /IL-1F8 Antibody and earn rewards!

Have you used Human IL-36 beta /IL-1F8 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review