Home / IL-38/IL-1F10 / Human IL-38/IL-1F10 Biotinylated Antibody

Human IL-38/IL-1F10 Biotinylated Antibody

Catalog #: BAF2427
2 Citations Datasheet / COA / SDS
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BAF2427
Product Details
Citations (2)
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Human IL-38/IL-1F10 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑38/IL‑1F10 in Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant human (rh) IL-1ra and rhIL-1F5 is observed and less than 1% cross-reactivity with rhIL-1 alpha, rhIL-18, rhIL-1F6, rhIL-1F7, rhIL-1F8, and rhIL-1F9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL‑38/IL‑1F10
Cys2-Trp152
Accession # Q8WWZ1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑38/IL‑1F10

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-38/IL-1F10

Human Il-38/IL-1F10 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-1F10 shares 82% aa sequence identity with mouse IL-1F10. Human IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra and IL-1 beta, respectively.

Long Name
Interleukin 1 Family Member 10
Entrez Gene IDs
84639 (Human); 215274 (Mouse); 102125744 (Cynomolgus Monkey)
Alternate Names
FIL1- theta; FKSG75; IL1F10; IL-1HY2; IL38; IL-38; interleukin 1 family, member 10 (theta); UNQ6119/PRO20041

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Citations for Human IL-38/IL-1F10 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Interleukin-38 interacts with destrin/actin-depolymerizing factor in human keratinocytes
    Authors: D Talabot-Ay, L Mermoud, J Borowczyk, J Drukala, M Wolnicki, A Modarressi, WH Boehncke, N Brembilla, G Palmer
    PLoS ONE, 2019-11-26;14(11):e0225782.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
    Authors: van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA
    Proc. Natl. Acad. Sci. U.S.A., 2012-02-06;109(8):3001-5.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development

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