Human IL-4 QuantiGlo ELISA Kit
Human IL-4 QuantiGlo ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||||
---|---|---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
n | 20 | 20 | 20 | 20 | 40 | 40 | 40 | 40 |
Mean (pg/mL) | 2.38 | 10.1 | 125 | 1306 | 2.8 | 11.9 | 137 | 1400 |
Standard Deviation | 0.21 | 0.85 | 8.13 | 100 | 0.41 | 1 | 7.2 | 60 |
CV% | 8.8 | 8.4 | 6.5 | 7.7 | 14.6 | 8.4 | 5.3 | 4.3 |
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||||
---|---|---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
n | 20 | 20 | 20 | 20 | 40 | 40 | 40 | 40 |
Mean (pg/mL) | 3.63 | 18.9 | 177 | 1666 | 3.7 | 15.9 | 166 | 1547 |
Standard Deviation | 0.34 | 0.56 | 8.39 | 78.1 | 0.43 | 1.3 | 13 | 117 |
CV% | 9.4 | 3 | 4.7 | 4.7 | 11.6 | 8.2 | 7.8 | 7.6 |
Recovery
The recovery of human IL-4 spiked to three different levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=9) | 103 | 87-115 |
Citrate Plasma (n=6) | 91 | 83-100 |
EDTA Plasma (n=6) | 92 | 78-106 |
Heparin Plasma (n=6) | 92 | 82-102 |
Serum (n=6) | 93 | 76-109 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: IL-4
IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 150 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20-40 minutes on the benchtop.
- Determine the RLU of each well using a luminometer.
FAQs
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