Human IL-4I1 Antibody

Catalog #: MAB56843 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB56843-100
MAB56843-SP

Detection of Human IL‑4I1 by Western Blot.

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All IL-4I1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-4I1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-4I1 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 1006231

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human IL-4I1
Met1-His567
Accession # Q96RQ9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑4I1 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM of PMA for 24 hours followed by 10 μg/mL of LPS for 3 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human IL-4I1 Monoclonal Antibody (Catalog # MAB56843) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-4I1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry

Detection of IL-4l1 antibody in THP-1 Human Cell Line antibody by Flow Cytometry.

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Detection of IL-4l1 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human cell line was stained with Mouse Anti-Human IL-4l1 Monoclonal Antibody (Catalog # MAB56843, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4I1

Interleukin 4 induced protein 1 (IL-4I1), also known as protein FIG-1 and L-amino acid oxidase, is encoded by a B-cell IL-4-inducible gene, FIG1, and is highly expressed in primary metastinal B-cell lymphomas (1-4). It belongs to the flavin monoamine oxidase family, FIG1 subfamily. Enzymological characterization reveals that IL-4I1 has L-amino acid oxidase activity with preference toward aromatic amino acids. Studies have shown that hIL-4I1 inhibited the proliferation of CD3‑stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells (5). Its inhibitory effect was dependent on enzymatic activity and H₂O₂ production. Its restricted expression to lymphoid tissues indicates that it may play an important function in the immune system (1, 4).

References

Chu, C.C. and W.E. Paul. (1997) Proc. Natl. Acad. Sci. USA 94:2507.
Mason, J.M. et al. (2004) J. Immunol. 173:4561.
Chavan, S.S. et al. (2002) Biochim. Biophys. Acta. 1576:70.
Copie-Bergman, C. et al. (2003) Blood 101:2756.
Boulland, M.L. et al. (2007) Blood 110:220.

Long Name

Interleukin 4-induced Protein-1

Entrez Gene IDs

259307 (Human); 14204 (Mouse); 308575 (Rat); 102115096 (Cynomolgus Monkey)

Alternate Names

Fig-1 protein; FIG1; hFIG1; IL4I1; IL-4I1; IL4-induced protein 1; interleukin 4 induced 1; interleukin four induced 1; LAAO; L-amino-acid oxidase; LAO; UNQ636/PRO1265