Human IL-6R alpha ELISA Kit - Quantikine

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Human IL-6 R alpha ELISA Standard Curve
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Citations (45)
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Human IL-6R alpha Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (50 uL)
Sensitivity
15.1 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human IL-6 sR
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-6 sR Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-6 sR in cell culture supernates, serum, plasma, and urine. It contains recombinant human IL-6 sR produced in Sf 21 cells and antibodies raised against this protein. This kit has been shown to accurately quantitate the recombinant soluble IL-6 R. Results obtained using natural IL-6 sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-6 sR. Since the measurement of human IL-6 sR by this immunoassay is insensitive to added recombinant human IL-6, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples ( i.e. the total amount of free receptor plus the total amount of receptor bound to IL-6).

Recovery

The recovery of IL-6 sR spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 85-97
Citrate Plasma (n=4) 93 86-102
EDTA Plasma (n=4) 93 76-107
Heparin Plasma (n=4) 101 87-118
Serum (n=4) 93 86-110
Urine (n=4) 86 64-98

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of natural or recombinant human IL-6 sR were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the as
Human IL-6 R alpha ELISA Linearity

Scientific Data

Human IL-6 R alpha ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6R alpha

IL-6 R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis.

Long Name:
Interleukin 6 Receptor alpha
Entrez Gene IDs:
3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names:
CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 3 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-6R alpha Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. IL-6 trans-signaling in a humanized mouse model of scleroderma
    Authors: Odell, ID;Agrawal, K;Sefik, E;Odell, AV;Caves, E;Kirkiles-Smith, NC;Horsley, V;Hinchcliff, M;Pober, JS;Kluger, Y;Flavell, RA;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Circulating Microvesicles in Association with the NLRP3 Inflammasome in Coronary Thrombi from STEMI Patients
    Authors: V Bratseth, J Nordeng, R Helseth, S Solheim, S Åkra, H Arnesen, G Chiva-Blan, I Seljeflot
    Biomedicines, 2022-09-05;10(9):.
    Species: Human
    Sample Types: Plasma
  3. Serum and Vascular Stiffness Biomarkers Associated with the Severity of Degenerative Aortic Valve Stenosis and Cardiovascular Outcomes
    Authors: J Baran, ? Niewiara, J Podolec, M Siedli?ski, E Józefczuk, A Bernacik, R Badacz, T Przew?ocki, P Pieni??ek, K ?mudka, J Legutko, A Kab?ak-Zie
    Oncogene, 2022-06-17;9(6):.
    Species: Human
    Sample Types: Serum
  4. Evaluating immune response and metabolic related biomarkers pre-allogenic hematopoietic stem cell transplant in acute myeloid leukemia
    Authors: S Siamakpour, F Cao, J Lyu, Y Ren, AB Nixon, J Xie, AT Bush, MD Starr, JR Bain, MJ Muehlbauer, O Ilkayeva, V Byers Krau, JL Huebner, NJ Chao, AD Sung
    PLoS ONE, 2022-06-14;17(6):e0268963.
    Species: Human
    Sample Types: Plasma
  5. Co-Occurrence of Interleukin-6 Receptor Asp358Ala Variant and High Plasma Levels of IL-6: An Evidence of IL-6 Trans-Signaling Activation in Deep Vein Thrombosis (DVT) Patients
    Authors: R Salemi, G Gattuso, B Tomasello, A Lavoro, A Gaudio, M Libra, SS Signorelli, S Candido
    Biomolecules, 2022-05-10;12(5):.
    Species: Human
    Sample Types: Plasma
  6. Dexmedetomidine attenuates oxygen-glucose deprivation/ reperfusion-induced inflammation through the miR-17-5p/ TLR4/ NF-kappaB axis
    Authors: L Suo, M Wang
    BMC anesthesiology, 2022-04-29;22(1):126.
    Species: Rat
    Sample Types:
  7. Cellular Response against Oxidative Stress, a Novel Insight into Lupus Nephritis Pathogenesis
    Authors: CD Ene, SR Georgescu, M Tampa, C Matei, CI Mitran, MI Mitran, MN Penescu, I Nicolae
    Journal of personalized medicine, 2021-07-22;11(8):.
    Species: Human
    Sample Types: Serum
  8. The Inflammasome Signaling Pathway Is Actively Regulated and Related to Myocardial Damage in Coronary Thrombi from Patients with STEMI
    Authors: J Nordeng, H Schandiz, S Solheim, S Åkra, P Hoffman, B Roald, B Bendz, H Arnesen, R Helseth, I Seljeflot
    Mediators of Inflammation, 2021-05-27;2021(0):5525917.
    Species: Human
    Sample Types: Serum
  9. Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma
    Authors: CD Ene, MN Penescu, SR Georgescu, M Tampa, I Nicolae
    Metabolites, 2020-12-27;11(1):.
    Species: Human
    Sample Types: Serum
  10. Inflammatory biomarkers, geriatric assessment, and treatment outcomes in acute myeloid leukemia
    Authors: KP Loh, JA Tooze, BJ Nicklas, SB Kritchevsk, JD Williamson, LR Ellis, BL Powell, TS Pardee, NG Goyal, HD Klepin
    J Geriatr Oncol, 2019-04-05;0(0):.
    Species: Human
    Sample Types: Plasma
  11. Management of Juvenile Idiopathic Arthritis in ABO-incompatible Kidney Transplantation: A Case Report
    Authors: S Ishikawa, M Tasaki, T Kuroda, D Kobayashi, K Saito, Y Nakagawa, M Ikeda, K Takahashi, Y Tomita
    Transplant. Proc., 2018-04-01;50(3):869-872.
    Species: Human
    Sample Types: Serum
  12. Adipogenic differentiation of MSC alters their immunomodulatory properties in a tissue-specific manner
    Authors: H Munir, LS Ward, L Sheriff, S Kemble, S Nayar, F Barone, GB Nash, HM McGettrick
    Stem Cells, 2017-04-24;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Inhibitory Mechanism of the Outer Membrane Growth of Chronic Subdural Hematomas
    Authors: Koji Osuka
    J. Neurotrauma, 2017-02-27;0(0):.
    Species: Human
    Sample Types: Chronic Subdural Hematoma Fluids
  14. Prevalent peripheral arterial disease and inflammatory burden
    BMC Geriatr, 2016-12-09;16(1):213.
    Species: Human
    Sample Types: Serum
  15. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016-11-15;90(0):88-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Inflammatory Markers and the Risk of Hip and Vertebral Fractures in Men: the Osteoporotic Fractures in Men (MrOS)
    J Bone Miner Res, 2016-07-25;0(0):.
    Species: Human
    Sample Types: Serum
  17. Associations of total and free 25OHD and 1,25(OH)2D with serum markers of inflammation in older men.
    Authors: P Srikanth, R F Chun, M Hewison, J S Adams, R Bouillon, D Vandersch, N Lane, P M Cawthon, T Dam, E Barrett-C, L B Daniels, J M Shikany, M L Stefanick, J A Cauley, E S Orwoll, C M Nielson
    Osteoporosis International : a Journal Established as Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the Usa, 2016-02-23;0(0):1433-2965.
    Species: Human
    Sample Types: Serum
  18. A Novel IL6 Antibody Sensitizes Multiple Tumor Types to Chemotherapy Including Trastuzumab-Resistant Tumors.
    Authors: Zhong H, Davis A, Ouzounova M, Carrasco R, Chen C, Breen S, Chang Y, Huang J, Liu Z, Yao Y, Hurt E, Moisan J, Fung M, Tice D, Clouthier S, Xiao Z, Wicha M, Korkaya H, Hollingsworth R
    Cancer Res, 2016-01-07;76(2):480-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFalpha-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction.
    Authors: Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, Usami I, Sakamoto S, Abe H, Watanabe T, Yamori T, Shibasaki M, Nomoto A
    PLoS ONE, 2015-03-18;10(3):e0119415.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Blood stem cell fate regulation by Delta-1-mediated rewiring of IL-6 paracrine signaling.
    Authors: Csaszar, Elizabet, Wang, Weijia, Usenko, Tatiana, Qiao, Wenlian, Delaney, Colleen, Bernstein, Irwin D, Zandstra, Peter W
    Blood, 2013-11-15;123(5):650-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
    Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
    J Neuroinflammation, 2013-02-21;10(0):29.
    Species: Human
    Sample Types: Serum
  22. Bruton tyrosine kinase inhibition is a novel therapeutic strategy targeting tumor in the bone marrow microenvironment in multiple myeloma.
    Authors: Tai Y, Chang B, Kong S, Fulciniti M, Yang G, Calle Y, Hu Y, Lin J, Zhao J, Cagnetta A, Cea M, Sellitto M, Zhong M, Wang Q, Acharya C, Carrasco D, Buggy J, Elias L, Treon S, Matsui W, Richardson P, Munshi N, Anderson K
    Blood, 2012-06-11;120(9):1877-87.
    Species: Human
    Sample Types: Recombinant Protein
  23. Inflammatory markers and the risk of hip fracture: the Women's Health Initiative.
    Authors: Barbour KE, Boudreau R, Danielson ME, Youk AO, Wactawski-Wende J, Greep NC, LaCroix AZ, Jackson RD, Wallace RB, Bauer DC, Allison MA, Cauley JA
    J. Bone Miner. Res., 2012-05-01;27(5):1167-76.
    Species: Human
    Sample Types: Serum
  24. Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
    Authors: Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E
    Clin. Exp. Immunol., 2010-10-05;162(3):500-9.
    Species: Human
    Sample Types: Milk
  25. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009-11-15;88(9):1109-16.
    Species: Human
    Sample Types: Urine
  26. Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target.
    Authors: Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC
    Cancer Cell, 2009-10-06;16(4):309-23.
    Species: Human
    Sample Types: Serum
  27. Daily family assistance and inflammation among adolescents from Latin American and European backgrounds.
    Authors: Fuligni AJ, Telzer EH, Bower J, Irwin MR, Kiang L, Cole SW
    Brain Behav. Immun., 2009-03-09;23(6):803-9.
    Species: Human
    Sample Types: Plasma
  28. Soluble and membrane levels of molecules involved in the interaction between clonal plasma cells and the immunological microenvironment in multiple myeloma and their association with the characteristics of the disease.
    Authors: Perez-Andres M, Almeida J, Martin-Ayuso M, De Las Heras N, Moro MJ, Martin-Nunez G, Galende J, Cuello R, Abuin I, Moreno I, Dominguez M, Hernandez J, Mateo G, San Miguel JF, Orfao A
    Int. J. Cancer, 2009-01-15;124(2):367-75.
    Species: Human
    Sample Types: Plasma
  29. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
    Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
    Cancer Res., 2009-01-01;69(1):329-37.
    Species: Human
    Sample Types: Serum
  30. Interleukin-6 and soluble interleukin-6 receptor levels as markers of disease extent and prognosis in neuroblastoma.
    Authors: Egler RA, Burlingame SM, Nuchtern JG, Russell HV
    Clin. Cancer Res., 2008-11-01;14(21):7028-34.
    Species: Human
    Sample Types: Plasma
  31. Improved prediction of disease relapse after radical prostatectomy through a panel of preoperative blood-based biomarkers.
    Authors: Shariat SF, Karam JA, Walz J, Roehrborn CG, Montorsi F, Margulis V, Saad F, Slawin KM, Karakiewicz PI
    Clin. Cancer Res., 2008-06-15;14(12):3785-91.
    Species: Human
    Sample Types: Plasma
  32. Inflammatory markers and incident fracture risk in older men and women: the Health Aging and Body Composition Study.
    Authors: Cauley JA, Danielson ME, Boudreau RM, Forrest KY, Zmuda JM, Pahor M, Tylavsky FA, Cummings SR, Harris TB, Newman AB
    J. Bone Miner. Res., 2007-07-01;22(7):1088-95.
    Species: Human
    Sample Types: Serum
  33. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
    Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
    AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):886-90.
    Species: Human
    Sample Types: Plasma
  34. Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
    Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
    Blood, 2007-06-13;110(6):1748-55.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Functional characterization of a soluble gp130 isoform and its therapeutic capacity in an experimental model of inflammatory arthritis.
    Authors: Richards PJ, Nowell MA, Horiuchi S, McLoughlin RM, Fielding CA, Grau S, Yamamoto N, Ehrmann M, Rose-John S, Williams AS, Topley N, Jones SA
    Arthritis Rheum., 2006-05-01;54(5):1662-72.
    Species: Human
    Sample Types: Serum
  36. Effect of influenza vaccine on markers of inflammation and lipid profile.
    Authors: Tsai MY, Hanson NQ, Straka RJ, Hoke TR, Ordovas JM, Peacock JM, Arends VL, Arnett DK
    J. Lab. Clin. Med., 2005-06-01;145(6):323-7.
    Species: Human
    Sample Types: Plasma
  37. Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images.
    Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005-04-01;99(4):411-8.
    Species: Human
    Sample Types: Synovial Fluid
  38. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
    Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
    Transpl. Int., 2005-02-01;18(2):177-85.
    Species: Human
    Sample Types: Plasma
  39. Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure.
    Authors: Hirota H, Izumi M, Hamaguchi T, Sugiyama S, Murakami E, Kunisada K, Fujio Y, Oshima Y, Nakaoka Y, Yamauchi-Takihara K
    Heart Vessels, 2004-09-01;19(5):237-41.
    Species: Human
    Sample Types: Plasma
  40. Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
    Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
    J. Virol., 2003-02-01;77(3):1727-37.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Serum
  41. Effects of IL-6 variants in multiple myeloma: growth inhibition and induction of apoptosis in primary cells.
    Authors: Petrucci MT, Ricciardi MR, Gregorj C, Ciapponi L, Savino R, Ciliberto G, Tafuri A
    Leuk. Lymphoma, 2002-12-01;43(12):2369-75.
    Species: Human
    Sample Types: Serum
  42. Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response.
    Authors: Carstanjen D, Regenfus M, Muller C, Salama A
    Exp. Hematol., 2001-07-01;29(7):812-21.
    Species: Human
    Sample Types: Plasma
  43. Ovariectomy does not induce osteopenia through interleukin-6 in rhesus monkeys (Macaca mulatta).
    Authors: Keller ET, 49536, Binkley NC, Stebler BA, Hall DM, Johnston GM, Zhang J, Ershler WB
    CpG Type A Induction of an Early Protective Environment in Experimental Multiple Sclerosis, 2000-01-01;26(1):55-62.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Bone Marrow Fluid
  44. Discriminant power of combined cerebrospinal fluid tau protein and of the soluble interleukin-6 receptor complex in the diagnosis of Alzheimer's disease.
    Authors: Hampel H, Teipel SJ, Padberg F, Haslinger A, Riemenschneider M, Schwarz MJ, Kotter HU, Scheloske M, Buch K, Stubner S, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ
    Brain Res., 1999-03-27;823(1):104-12.
    Species: Human
    Sample Types: CSF
  45. Interleukin-6 and the soluble IL-6 receptor are decreased in cerebrospinal fluid of geriatric patients with major depression: no alteration of soluble gp130.
    Authors: Stubner S, Schon T, Padberg F, Teipel SJ, Schwarz MJ, Haslinger A, Buch K, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ, Hampel H
    Neurosci. Lett., 1999-01-15;259(3):145-8.
    Species: Human
    Sample Types: CSF

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Human IL-6R alpha Quantikine ELISA Kit
By Anonymous on 03/01/2022
Sample Tested: acute myeloid leukemia,Renal Cell Carcinoma

Human IL-6R alpha Quantikine ELISA Kit
By Anonymous on 01/06/2022
Sample Tested: Heart (adipose) tissue

Human IL-6R alpha Quantikine ELISA Kit
By Anonymous on 09/30/2021
Sample Tested: Blood mononuclear cells (PBMCs)

Human IL-6 R alpha Quantikine ELISA Kit
By Jessica Fernando on 12/04/2017
Sample Tested: Serum