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Human IL-6R alpha Antibody

Catalog #: AF-227-NA
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-227-NA
AF-227-SP
Detection of Recombinant Human IL-6R alpha by Western Blot.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-6R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6R alpha in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
Leu20-Asp358
Accession # P08887
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL-6R alpha-mediated inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.2-1.6 µg/mL in the presence of 30 ng/mL Recombinant Human IL-6R alpha and 30 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human IL-6R alpha antibody by Western Blot. View Larger

Detection of Recombinant Human IL-6R alpha by Western Blot. Western blot shows 5 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR), Recombinant Mouse IL-6R alpha (Catalog # 1830-SR) and Recombinant Rat IL-6 R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization IL-6R alpha Enhancement of IL‑6‑dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody. View Larger

IL-6R alpha Enhancement of IL‑6‑dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody. Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA). The ND50 is typically 0.2-1.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

References
  1. Yamasaki et al. (1988) Science 241:825.
  2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
  3. Hibi et al. (1990) Cell 63:1149.
  4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
  5. Novick et al. (1989) J. Exp. Med. 170:1409.
  6. Yasukawa et al. (1990) J. Biochem. 108:673.
  7. Saito et al. (1991) J. Immunology 147:168.
Long Name
Interleukin 6 Receptor alpha
Entrez Gene IDs
3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names
CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

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Citations for Human IL-6R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. NF-kappa B non-cell-autonomously regulates cancer stem cell populations in the basal-like breast cancer subtype
    Authors: Mizuki Yamamoto, Yuu Taguchi, Taku Ito-Kureha, Kentaro Semba, Noritaka Yamaguchi, Jun-ichiro Inoue
    Nature Communications
  2. An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
    Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
    J Neuroinflammation, 2013-02-21;10(0):29.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
    Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
    Haematologica, 2011-05-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
    Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
    J. Immunol., 2011-03-02;186(7):4175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy.
    Authors: Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H
    Circulation, 2003-02-18;107(6):798-802.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Protection of CLL B cells by a follicular dendritic cell line is dependent on induction of Mcl-1.
    Authors: Pedersen IM, Kitada S, Leoni LM, Zapata JM, Karras JG, Tsukada N, Kipps TJ, Choi YS, Bennett F, Reed JC
    Blood, 2002-09-01;100(5):1795-801.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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