Human IL-6R alpha Biotinylated Antibody Summary
Leu20-Asp358
Accession # P08887
Applications
Human IL-6R alpha Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6R alpha
The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).
A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).
- Yamasaki et al. (1988) Science 241:825.
- Baumann et al. (1990) J. Biol. Chem. 265:19853.
- Hibi et al. (1990) Cell 63:1149.
- Schooltink et al. (1991) Eur. J. Biochem. 277:659.
- Novick et al. (1989) J. Exp. Med. 170:1409.
- Yasukawa et al. (1990) J. Biochem. 108:673.
- Saito et al. (1991) J. Immunology 147:168.
Product Datasheets
Citations for Human IL-6R alpha Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis.
Authors: Komori M, Blake A, Greenwood M et al.
Ann Neurol
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Interleukin-6 controls recycling and degradation, but not internalization of its receptors
Authors: CM Flynn, B Kespohl, T Daunke, Y Garbers, S Düsterhöft, S Rose-John, J Haybaeck, J Lokau, S Aparicio-S, C Garbers
The Journal of Biological Chemistry, 2021-02-19;0(0):100434.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Exploitation of Elevated Extracellular ATP to Specifically Direct Antibody to Tumor Microenvironment
Authors: F Mimoto, K Tatsumi, S Shimizu, S Kadono, K Haraya, M Nagayasu, Y Suzuki, E Fujii, M Kamimura, A Hayasaka, H Kawauchi, K Ohara, M Matsushita, T Baba, H Susumu, T Sakashita, T Muraoka, K Aso, H Katada, E Tanaka, K Nakagawa, M Hasegawa, M Ayabe, T Yamamoto, S Tanba, T Ishiguro, T Kamikawa, T Nambu, T Kibayashi, Y Azuma, Y Tomii, A Kato, K Ozeki, N Murao, M Endo, J Kikuta, M Kamata-Sak, M Ishii, K Hattori, T Igawa
Cell Reports, 2020-12-22;33(12):108542.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Capture -
Inhibitory FcgammaRIIb-Mediated Soluble Antigen Clearance from Plasma by a pH-Dependent Antigen-Binding Antibody and Its Enhancement by Fc Engineering.
Authors: Iwayanagi Y, Igawa T, Maeda A, Haraya K, Wada N, Shibahara N, Ohmine K, Nambu T, Nakamura G, Mimoto F, Katada H, Ito S, Tachibana T, Jishage K, Hattori K
J Immunol, 2015-08-28;195(7):3198-205.
Species: Human
Sample Types: Protein
Applications: ELISA Development -
A disintegrin and metalloprotease 17 dynamic interaction sequence, the sweet tooth for the human interleukin 6 receptor.
Authors: Dusterhoft S, Hobel K, Oldefest M, Lokau J, Waetzig G, Chalaris A, Garbers C, Scheller J, Rose-John S, Lorenzen I, Grotzinger J
J Biol Chem, 2014-04-30;289(23):16336-48.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa.
Authors: Wood MW, Breitschwerdt EB, Gookin JL
Infect. Immun., 2010-11-29;79(2):708-15.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-Fr -
trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
J. Immunol., 2002-11-01;169(9):5244-51.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
Immunity, 2001-06-01;14(6):705-14.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Proteolytic Origin of the Soluble Human IL-6R In Vivo and a Decisive Role of N-Glycosylation
Authors: S Riethmuell, P Somasundar, JC Ehlers, CW Hung, CM Flynn, J Lokau, M Agthe, S Dsterh”ft, Y Zhu, J Gr”tzinger, I Lorenzen, T Koudelka, K Yamamoto, U Pickhinke, R Wichert, C Becker-Pau, M R„disch, A Albrecht, M Hessefort, D Stahnke, C Unverzagt, S Rose-John, A Tholey, C Garbers
PLoS Biol, 2017-01-06;15(1):e2000080.
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Human IL-6 receptor alpha was measured in human plasma samples using an immunoassay comprising of this antibody and MAB227. This antibody was used as the detection and the MAB227 was used as the solid phase capture molecule.
